LOCUS       40924_12355        5452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMVEBNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Swirski RA, Van Den Berg D, Murphy AJ, Lambert CM, Friedberg EC, 
            Schimke RT.
  TITLE     Improvements in the Epstein-Barr-based shuttle vector system for 
            direct cloning in human tissue culture cells
  JOURNAL   Methods: A Companion to Methods in Enzymology 4, 133-142 (1992)
REFERENCE   3  (bases 1 to 5452)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 5452)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Methods: A 
            Companion to Methods in Enzymology 4, 133-142 (1992)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..5452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             798..2720
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(2824..2840)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3314..3418
                     /label=AmpR promoter
     CDS             3419..4276
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4450..5038
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5326..5347
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5362..5392
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5400..5416
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5424..5440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctg actctagagg atccccgggt acccggggat
      781 ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag
      841 gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag
      901 ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag
      961 gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa
     1021 aacgtccaag ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag
     1081 cgggaggggc aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg
     1141 caggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag
     1201 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag
     1261 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
     1321 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     1381 gggcaggagc aggaggaggg gcaggagcag gaggggcagg aggggcagga ggggcaggag
     1441 caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggagggg
     1501 caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga ggggcaggag
     1561 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggagggg
     1621 caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg gcaggagggg
     1681 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     1741 gaggggcagg agcaggaggg gcaggagcag gaggtggagg ccggggtcga ggaggcagtg
     1801 gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga ggccgccggg
     1861 gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg agaggtcgtg
     1921 gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc gggtctccac
     1981 cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt
     2041 ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag
     2101 agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg
     2161 atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca
     2221 acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag
     2281 aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga
     2341 cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac
     2401 cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa
     2461 gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt
     2521 tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg
     2581 tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg
     2641 aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg
     2701 agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat cgtattaaaa
     2761 gccgtgtatt cccccgcact aaagaataaa tccccagtag acctgcaggc atgcaagctt
     2821 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     2881 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     2941 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     3001 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     3061 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     3121 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3181 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     3241 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     3301 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3541 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3601 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3661 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3721 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3781 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3841 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3901 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3961 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4021 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4081 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4141 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4201 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4261 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     4621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5221 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5281 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5341 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5401 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg cc
//
