LOCUS       40924_12345        4912 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Eukaryotic expression vector pCMV8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Andersson S, Davis DL, Dahlback H, Jornvall H, Russell DW.
  TITLE     Cloning, structure, and expression of the mitochondrial cytochrome 
            P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme
  JOURNAL   J. Biol. Chem. 264 (14), 8222-8229 (1989)
  PUBMED    2722778
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Andersson S, Davis DL, Dahlback H, Jornvall H, Russell DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2000) Molecular Genetics, University of Texas 
            Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., 
            Dallas, TX 75235, USA
REFERENCE   3  (bases 1 to 4912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "1989"; volume: "264"; issue: "14"; pages: "8222-8229"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-FEB-2000) Molecular Genetics, University of Texas Southwestern 
            Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        699..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1073..1301
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     polyA_signal    1348..1969
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2056..2266
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2313..2331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2346..2362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2370..2386)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2394..2424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2439..2460)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2747..3335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        complement(4314..4418)
                     /label=AmpR promoter
     rep_origin      4445..4873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgatc tatacattga
      181 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
      241 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      301 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      541 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      721 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
      841 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      901 ctcgtttagt gaaccgtcag aattctgttg ggctcgcggt tgaggacaaa ctcttcgcgg
      961 tctttccagt actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag
     1021 ggacctgagc gagtccgcat cgaccggatc ggaaaacctc tcgactgttg gggtgagtac
     1081 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
     1141 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
     1201 gaaaagacaa tctttttgtt gtcaagcttg aggtgtggca agcttgagat ctggccatac
     1261 acttgagtga caatgacatc cactttgcct ttctctccac aagtgtccac tcccaagtcc
     1321 aactgcaggt cgactctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct
     1381 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     1441 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     1501 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     1561 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     1621 attctcctgc ctcagcctcc cgagttgttg ggattccagc atgcatgacc aggctcagct
     1681 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     1741 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     1801 ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag gaggacgtcc
     1861 agacacagca taggctacct gccatggccc aaccggtggg acatttgagt tgcttgcttg
     1921 gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg ggtacgcggc
     1981 cagcttctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2041 agaagtatct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2101 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     2161 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     2221 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     2281 ctcgaggaac tgaaaaacca gaaagttaat tccctatagt gagtcgtatt aaattcgtaa
     2341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2701 ggccagcaaa aggccaggaa ccgtaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2761 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2821 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2881 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2941 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3001 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3061 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3121 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3181 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3241 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3301 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3361 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3421 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3481 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3541 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3601 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac cacgctcacc
     3661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4081 atagtgtatg cggcgagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4441 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4501 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     4621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4741 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4801 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4861 tttaacgcga attttaacaa aatattaaca aaatattaac gtttacaatt tc
//
