LOCUS       40924_12340        4775 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Eukaryotic expression vector pCMV7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Andersson S, Davis DL, Dahlback H, Jornvall H, Russell DW.
  TITLE     Cloning, structure, and expression of the mitochondrial cytochrome 
            P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme
  JOURNAL   J. Biol. Chem. 264 (14), 8222-8229 (1989)
  PUBMED    2722778
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Andersson S, Davis DL, Dahlback H, Jornvall H, Russell DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2000) Molecular Genetics, University of Texas 
            Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., 
            Dallas, TX 75235, USA
REFERENCE   3  (bases 1 to 4775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "1989"; volume: "264"; issue: "14"; pages: "8222-8229"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-FEB-2000) Molecular Genetics, University of Texas Southwestern 
            Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        699..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          936..1165
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     polyA_signal    1211..1832
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        complement(2176..2194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2209..2225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2233..2249)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2257..2287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2302..2323)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2610..3198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3666..4044)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(4177..4281)
                     /label=AmpR promoter
     rep_origin      4308..4736
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgatc tatacattga
      181 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
      241 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      301 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      541 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      721 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
      841 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      901 ctcgtttagt gaaccgtcag aattcgactg ttggggtgag tactccctct caaaagcggg
      961 catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg
     1021 gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt
     1081 gttgtcaagc ttgaggtgtg gcaggctaga gatctggcca tacacttgag tgacaatgac
     1141 atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgactct
     1201 agaggatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     1261 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     1321 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     1381 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     1441 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     1501 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     1561 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     1621 ctacccacct tggcctccca aattgctggg attacagggt gaaccactgc tcccttccct
     1681 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     1741 cctgccatgg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     1801 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcttc tgtggaatgt
     1861 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1921 gcatctcaat tagtcagcaa ccaggtgtgg aaaagtcccc aggctcccca gcaggcagaa
     1981 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     2041 tcccgccctt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     2101 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa
     2161 ccagaaagtt aattccctat agtgagtcgt attaaattcg taatcatggt catagctgtt
     2221 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2281 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2341 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2401 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2581 gaaccgtaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     2821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     3061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc gttaatagtt
     3661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4081 tgagatccag ttcgatgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4141 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     4321 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4381 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4441 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca
     4501 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4561 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4621 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4681 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4741 caaaatatta acaaaatatt aacgtttaca atttc
//
