LOCUS       pCMV6-XL5-DDK-Hi        4626 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) mammalian transient expression vector for 
            fusing an ORF to C-terminal FLAG(R) and 6xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV6-XL5-DDK-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..4626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    979..1072
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1079..1102
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1109..1126
                     /label=6xHis
                     /note="6xHis affinity tag"
     primer_bind     complement(1179..1195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1206..1828
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1857..2186
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(2434..3022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3196..4053)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4054..4158)
                     /label=AmpR promoter
     rep_origin      4185..4613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccgcga attcgtcgac tggatctggt accgaggaga tccgccgccg
     1021 cgatcgccgg cgcgccagat cccaagctta actagctagc ggaccgacgc gttcaggcga
     1081 ctacaaggat gacgacgata agggatctca tcatcaccat caccattaat gagatctggt
     1141 accgatatca agcttgtcga ctctagattg cggccgcggt catagctgtt tcctgaacag
     1201 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
     1261 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
     1321 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
     1381 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
     1441 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
     1501 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
     1561 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
     1621 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
     1681 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
     1741 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
     1801 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
     1861 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1921 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1981 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     2041 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     2101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     2161 tttttggagg cctaggcttt tgcaaaaagc tcctcgactg cattaatgaa tcggccaacg
     2221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3121 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3181 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3241 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3301 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3361 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3421 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3481 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3541 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3601 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3661 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3721 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3781 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3841 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3901 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3961 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4021 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4081 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4141 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     4201 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4261 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4321 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4381 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4441 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4501 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4561 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4621 aatatt
//
