LOCUS       pCMV6-AC-FC-S.        6661 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) mammalian expression vector for fusing an ORF 
            to an N-terminal secretory signal sequence and a C-terminal murine 
            Fc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV6-AC-FC-S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..6661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     1020..1079
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     misc_feature    1080..1147
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1148..1843
                     /label=Fc tag
                     /note="CH2 and CH3 domains of the mouse IgG2a heavy chain
                     plus the hinge region"
     polyA_signal    1893..2515
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2544..2873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2940..3731
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3908..4041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4078..4094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4102..4118)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4126..4156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4171..4192)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4480..5068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5242..6099)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6100..6204)
                     /label=AmpR promoter
     rep_origin      6231..6661
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccacca
     1021 tgtacaggat gcaactcctg tcttgcattg cactaagtct tgcacttgtc acgaattcgg
     1081 cgatcgccgg cgcgccagat ctcaagctta actagttagc ggaccgacgc gtacgcggcc
     1141 gctcgagccc agagggccca caatcaagcc ctgtcctcca tgcaaatgcc cagcacctaa
     1201 cctcttgggt ggaccatccg tcttcatctt ccctccaaag atcaaggatg tactcatgat
     1261 ctccctgagc cccatagtca catgtgtggt ggtggatgtg agcgaggatg acccagatgt
     1321 ccagatcagc tggtttgtga acaacgtgga agtacacaca gctcagacac aaacccatag
     1381 agaggattac aacagtactc tccgggtggt cagtgccctc cccatccagc accaggactg
     1441 gatgagtggc aaggagttca aatgcaaggt caacaacaaa gacctcccag cgcccatcga
     1501 gagaaccatc tcaaaaccca aagggtcagt aagagctcca caggtatatg tcttgcctcc
     1561 accagaagaa gagatgacta agaaacaggt cactctgacc tgcatggtca cagacttcat
     1621 gcctgaagac atttacgtgg agtggaccaa caacgggaaa acagagctaa actacaagaa
     1681 cactgaacca gtcctggact ctgatggttc ttacttcatg tacagcaagc tgagagtgga
     1741 aaagaagaac tgggtggaaa gaaatagcta ctcctgttca gtggtccacg agggtctgca
     1801 caatcaccac acgactaaga gcttctcccg gactccgggt aaatgagttt aaacggccgg
     1861 ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac ccctccccag
     1921 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
     1981 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
     2041 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
     2101 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
     2161 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
     2221 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
     2281 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
     2341 cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac cagcaggagg
     2401 acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat ttgagttgct
     2461 tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt gaattgggta
     2521 cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2581 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2701 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2761 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     2821 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     2881 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     2941 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     3001 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     3061 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3121 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3181 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3241 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3301 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3361 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3421 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3481 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3541 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggccgaccgc tatcaggaca
     3601 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3661 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3721 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3781 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3841 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3901 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4021 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4201 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4261 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4321 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4381 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4441 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4501 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4561 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4621 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4681 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4741 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4801 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4861 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4921 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4981 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5041 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5101 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5161 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5221 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5281 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5341 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5401 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5461 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5521 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5581 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5641 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5701 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5761 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5821 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5881 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5941 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6001 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6061 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     6241 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6301 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6361 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6421 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6481 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6541 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6601 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6661 t
//
