LOCUS       pCMV6-AC-DDK-His        5956 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) mammalian expression vector for fusing an ORF 
            to C-terminal FLAG(R) and 6xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV6-AC-DDK-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..5956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    979..1087
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1088..1111
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1127..1144
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1191..1813
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1842..2171
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2238..3029
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3206..3339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3376..3392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3400..3416)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3424..3454)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3469..3490)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3778..4363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4537..5394)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5395..5499)
                     /label=AmpR promoter
     rep_origin      5526..5956
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccgg cgcgccagat ctcaagctta actagctagc ggaccgacgc gtacgcggcc
     1081 gctcgaggat tacaaggatg acgacgataa ggcaaatgat atcctgcatc accatcacca
     1141 tcacgtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc gggtggcatc
     1201 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     1261 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     1321 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     1381 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     1441 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     1501 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     1561 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     1621 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat
     1681 aactatacca gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg
     1741 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga
     1801 tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg
     1861 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1921 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     1981 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2041 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2161 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     2221 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2281 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2341 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2401 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     2461 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     2521 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     2581 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     2641 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     2701 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     2761 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     2821 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     2881 ccgaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     2941 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3001 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3061 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3121 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3181 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     3241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3301 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     3361 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3421 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3481 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3541 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3601 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3661 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3721 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3781 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3841 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3901 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3961 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4021 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4081 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4141 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4201 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4261 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     4321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5521 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5581 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5641 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5701 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5761 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5821 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5881 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5941 atttaacgcg aatttt
//
