LOCUS       pCMV6-A-EM7-BSD.        5572 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) mammalian vector for expressing an untagged 
            ORF, with a blasticidin resistance marker that can also be used in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV6-A-EM7-BSD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..5572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    979..1110
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1137..1759
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1788..2117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2176..2223
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2242..2637
                     /label=BSD
                     /note="blasticidin S deaminase"
     polyA_signal    2819..2952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2989..3005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3013..3029)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3037..3067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3082..3103)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3391..3979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4153..5010)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5011..5115)
                     /label=AmpR promoter
     rep_origin      5142..5572
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccgg cgcgccagat ctcaagctta actagttagc ggaccgacgc gttaagcggc
     1081 cgcactcgag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca gatcccgggt
     1141 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     1201 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     1261 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     1321 gtagggcctg cggggtctat tgggaaccaa gcaggagtgc agtggcacaa tcttggctca
     1381 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     1441 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     1501 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     1561 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     1621 taaaataact ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc
     1681 aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc
     1741 agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag
     1801 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1861 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1921 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1981 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2041 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2101 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     2161 agacacgtac gccgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     2221 gacaaggtga ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt
     2281 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     2341 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     2401 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     2461 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     2521 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     2581 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     2641 ctgcagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     2701 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     2761 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     2821 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2881 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     2941 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     3001 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3061 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3121 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3181 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4261 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4921 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     5161 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5221 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5281 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5341 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5401 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5461 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5521 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
