LOCUS       V014615                 7063 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014615
VERSION     V014615
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             940..2118
                     /gene="A"
                     /label="Maturation protein A"
                     /note="Maturation protein A from Escherichia phage MS2.
                     Accession#: P03610"
     CDS             2148..2534
                     /label="MS2"
                     /note="bacteriophage MS2 coat protein"
     polyA_signal    2630..2854
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2900..3328
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3342..3671
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3738..4529
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4706..4839
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4876..4892)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4900..4916)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4924..4954)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4969..4990)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5278..5863)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6037..6894)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6895..6999)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgtaggtagc cggaattcca ttcctaggag gtttgacctg tgcgagcttt tagtaccctt
      961 gatagggaga acgagacctt cgtcccctcc gttcgcgttt acgcggacgg tgagactgaa
     1021 gataactcat tctctttaaa atatcgttcg aactggactc ccggtcgttt taactcgact
     1081 ggggccaaaa cgaaacagtg gcactacccc tctccgtatt cacggggggc gttaagtgtc
     1141 acatcgatag atcaaggtgc ctacaagcga agtgggtcat cgtggggtcg cccgtacgag
     1201 gagaaagccg gtttcggctt ctccctcgac gcacgctcct gctacagcct cttccctgta
     1261 agccaaaact tgacttacat cgaagtgccg cagaacgttg cgaaccgggc gtcgaccgaa
     1321 gtcctgcaaa aggtcaccca gggtaatttt aaccttggtg ttgctttagc agaggccagg
     1381 tcgacagcct cacaactcgc gacgcaaacc attgcgctcg tgaaggcgta cactgccgct
     1441 cgtcgcggta attggcgcca ggcgctccgc taccttgccc taaacgaaga tcgaaagttt
     1501 cgatcaaaac acgtggccgg caggtggttg gagttgcagt tcggttggtt accactaatg
     1561 agtgatatcc agggtgcata tgagatgctt acgaaggttc accttcaaga gtttcttcct
     1621 atgagagccg tacgtcaggt cggtactaac atcaagttag atggccgtct gtcgtatcca
     1681 gctgcaaact tccagacaac gtgcaacata tcgcgacgta tcgtgatatg gttttacata
     1741 aacgatgcac gtttggcatg gttgtcgtct ctaggtatct tgaacccact aggtatagtg
     1801 tgggaaaagg tgcctttctc attcgttgtc gactggctcc tacctgtagg taacatgctc
     1861 gagggcctta cggcccccgt gggatgctcc tacatgtcag gaacagttac tgacgtaata
     1921 acgggtgagt ccatcataag cgttgacgct ccctacgggt ggactgtgga gagacagggc
     1981 actgctaagg cccaaatctc agccatgcat cgaggggtac aatccgtatg gccaacaact
     2041 ggcgcgtacg taaagtctcc tttctcgatg gtccatacct tagatgcgtt agcattaatc
     2101 aggcaacggc tctctagata gagccctcaa ccggagtttg aagcatggct tctaacttta
     2161 ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact
     2221 tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg ttcacaggct tacaaagtaa
     2281 cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc
     2341 ctaaagtggc aacccagact gttggtggtg tagagcttcc tgtagccgca tggcgttcgt
     2401 acttaaatat ggaactaacc attccaattt tcgctacgaa ttccgactgc gagcttattg
     2461 ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcagca atcgcagcaa
     2521 actccggcat ctactaatag acgccggcca ttcgaattct gcagatatcc agcacagtgg
     2581 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2641 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2701 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2761 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2821 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2881 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2941 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3001 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3061 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3121 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3181 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3241 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3301 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3421 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3481 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3601 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3661 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     3721 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3781 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3841 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3901 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3961 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4021 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4081 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4141 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4201 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4261 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4321 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4381 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4441 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4501 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4561 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4621 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4681 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4741 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4801 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4861 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4921 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4981 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5041 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5101 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5161 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5701 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     5821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7021 acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
