LOCUS       62056_7205        5326 bp DNA     circular SYN 14-AUG-2021
DEFINITION  Cloning vector pCMV-mCherry, complete sequence.
ACCESSION   MZ220611
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Yang J, Lee J, Land MA, Lai S, Igoshin OA, St-Pierre F.
  TITLE     A synthetic circuit for buffering gene dosage variation between 
            individual mammalian cells
  JOURNAL   Nat Commun 12 (1), 4132 (2021)
  PUBMED    34226556
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Yang J, Lee J, Land MA, Lai S, Igoshin O, St-Pierre F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2021) Neuroscience, Baylor College of Medicine, 
            One Baylor Plaza, Suite S636, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 5326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2021"; volume: "12"; issue: "1"; pages: "4132"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2021) Neuroscience, Baylor College of Medicine, One Baylor 
            Plaza, Suite S636, Houston, TX 77030, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    611..658
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      987..1575
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        1750..2053
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2054..2257
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2742..3449
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    3457..3681
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3803..3924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3931..4386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4413..4517
                     /label=AmpR promoter
     promoter        4519..4876
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             join(4911..5326,1..376)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
       61 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
      121 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
      181 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
      241 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
      301 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
      361 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
      421 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
      481 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
      541 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
      601 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
      661 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
      721 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
      781 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
      841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
      901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1681 gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     1741 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     1801 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     1861 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1921 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1981 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2041 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2101 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2161 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2221 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
     2281 ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     2341 ccaccatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg
     2401 tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt
     2461 ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg
     2521 ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt
     2581 cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg
     2641 tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca
     2701 tcaccaagta caccagcgct aaggatccac cggtcgccac catggtgtcc aagggagaag
     2761 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtccacatg gagggcagcg
     2821 tgaatggcca cgagttcgag atcgagggcg aaggcgaggg acgtccctac gaaggcaccc
     2881 agaccgccaa actgaaggtg accaagggag gcccgttgcc ctttgcctgg gacatcttga
     2941 gcccccagtt catgtacggc tccaaggcct acgtgaagca cccggccgac attcccgact
     3001 acctgaagct gagcttcccc gaaggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3061 gcggagtggt gaccgtgacc caggactcca gcttgcagga cggcgagttc atctacaagg
     3121 tcaagctgcg cggcacgaac tttccctccg atggcccggt gatgcagaag aagaccatgg
     3181 gctgggaggc ttccagcgag cgcatgtatc ccgaggacgg cgctctgaag ggcgagatca
     3241 agcagcgcct gaagctgaag gatggaggcc actacgacgc cgaggtgaag accacctaca
     3301 aggccaagaa gcccgtgcaa ttgcccggcg cctacaacgt gaacatcaag ctggacatca
     3361 ccagccacaa cgaggactac accatcgtgg agcagtacga gcgtgccgaa ggacgtcata
     3421 gcacgggcgg catggatgag ctgtacaagt aagcttctgt gccttctagt tgccagccat
     3481 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3541 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3601 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3661 gggatgcggt gggctctatg ggcggccgcg actctagatc ataatcagcc ataccacatt
     3721 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3781 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3901 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3961 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     4021 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     4081 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4141 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     4201 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     4261 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     4321 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4381 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4441 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4501 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4621 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4681 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4741 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4801 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4861 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4921 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4981 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5041 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     5101 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5161 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5221 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5281 atacgcttga tccggctacc tgcccattcg accaccaagc gaaaca
//
