LOCUS       V014771                 9528 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014771
VERSION     V014771
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..237
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             312..1103
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1280..1413
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1450..1466)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1474..1490)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1498..1528)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1543..1564)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1852..2440)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2614..3471)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3472..3576)
                     /label="AmpR promoter"
     enhancer        3842..4221
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4222..4425
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4470..4488
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4530..7430
                     /label="RfxCas13d"
                     /note="RNA-targeting Type VI CRISPR protein from
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
     CDS             7437..7463
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             7491..8672
                     /gene="ALKBH5"
                     /label="RNA demethylase ALKBH5"
                     /note="RNA demethylase ALKBH5 from Homo sapiens.
                     Accession#: Q6P6C2"
     polyA_signal    8732..8956
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9002..9430
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        9444..9528
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
       61 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      181 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      241 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
      301 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
      361 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
      421 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
      481 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
      541 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
      601 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
      661 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
      721 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
      781 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
      841 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
      901 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
      961 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     1021 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     1081 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     1141 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1201 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1261 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1381 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2281 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3601 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3661 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3721 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3781 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3841 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3901 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3961 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4021 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4081 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4141 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4201 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4261 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4321 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4381 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     4441 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     4501 ggctagcgtt taaacttaag cttgccacca tgatcgaaaa aaaaaagtcc ttcgccaagg
     4561 gcatgggcgt gaagtccaca ctcgtgtccg gctccaaagt gtacatgaca accttcgccg
     4621 aaggcagcga cgccaggctg gaaaagatcg tggagggcga cagcatcagg agcgtgaatg
     4681 agggcgaggc cttcagcgct gaaatggccg ataaaaacgc cggctataag atcggcaacg
     4741 ccaaattcag ccatcctaag ggctacgccg tggtggctaa caaccctctg tatacaggac
     4801 ccgtccagca ggatatgctc ggcctgaagg aaactctgga aaagaggtac ttcggcgaga
     4861 gcgctgatgg caatgacaat atttgtatcc aggtgatcca taacatcctg gacattgaaa
     4921 aaatcctcgc cgaatacatt accaacgccg cctacgccgt caacaatatc tccggcctgg
     4981 ataaggacat tattggattc ggcaagttct ccacagtgta tacctacgac gaattcaaag
     5041 accccgagca ccatagggcc gctttcaaca ataacgataa gctcatcaac gccatcaagg
     5101 cccagtatga cgagttcgac aacttcctcg ataaccccag actcggctat ttcggccagg
     5161 cctttttcag caaggagggc agaaattaca tcatcaatta cggcaacgaa tgctatgaca
     5221 ttctggccct cctgagcgga ctggcgcact gggtggtcgc taacaacgaa gaagagtcca
     5281 ggatctccag gacctggctc tacaacctcg ataagaacct cgacaacgaa tacatctcca
     5341 ccctcaacta cctctacgac aggatcacca atgagctgac caactccttc tccaagaact
     5401 ccgccgccaa cgtgaactat attgccgaaa ctctgggaat caaccctgcc gaattcgccg
     5461 aacaatattt cagattcagc attatgaaag agcagaaaaa cctcggattc aatatcacca
     5521 agctcaggga agtgatgctg gacaggaagg atatgtccga gatcaggaaa aatcataagg
     5581 tgttcgactc catcaggacc aaggtctaca ccatgatgga ctttgtgatt tataggtatt
     5641 acatcgaaga ggatgccaag gtggctgccg ccaataagtc cctccccgat aatgagaagt
     5701 ccctgagcga gaaggatatc tttgtgatta acctgagggg ctccttcaac gacgaccaga
     5761 aggatgccct ctactacgat gaagctaata gaatttggag aaagctcgaa aatatcatgc
     5821 acaacatcaa ggaatttagg ggaaacaaga caagagagta taagaagaag gacgccccta
     5881 gactgcccag aatcctgccc gctggccgtg atgtttccgc cttcagcaaa ctcatgtatg
     5941 ccctgaccat gttcctggat ggcaaggaga tcaacgacct cctgaccacc ctgattaata
     6001 aattcgataa catccagagc ttcctgaagg tgatgcctct catcggagtc aacgctaagt
     6061 tcgtggagga atacgccttt ttcaaagact ccgccaagat cgccgatgag ctgaggctga
     6121 tcaagtcctt cgctagaatg ggagaaccta ttgccgatgc caggagggcc atgtatatcg
     6181 acgccatccg tattttagga accaacctgt cctatgatga gctcaaggcc ctcgccgaca
     6241 ccttttccct ggacgagaac ggaaacaagc tcaagaaagg caagcacggc atgagaaatt
     6301 tcattattaa taacgtgatc agcaataaaa ggttccacta cctgatcaga tacggtgatc
     6361 ctgcccacct ccatgagatc gccaaaaacg aggccgtggt gaagttcgtg ctcggcagga
     6421 tcgctgacat ccagaaaaaa cagggccaga acggcaagaa ccagatcgac aggtactacg
     6481 aaacttgtat cggaaaggat aagggcaaga gcgtgagcga aaaggtggac gctctcacaa
     6541 agatcatcac cggaatgaac tacgaccaat tcgacaagaa aaggagcgtc attgaggaca
     6601 ccggcaggga aaacgccgag agggagaagt ttaaaaagat catcagcctg tacctcaccg
     6661 tgatctacca catcctcaag aatattgtca atatcaacgc caggtacgtc atcggattcc
     6721 attgcgtcga gcgtgatgct caactgtaca aggagaaagg ctacgacatc aatctcaaga
     6781 aactggaaga gaagggattc agctccgtca ccaagctctg cgctggcatt gatgaaactg
     6841 cccccgataa gagaaaggac gtggaaaagg agatggctga aagagccaag gagagcattg
     6901 acagcctcga gagcgccaac cccaagctgt atgccaatta catcaaatac agcgacgaga
     6961 agaaagccga ggagttcacc aggcagatta acagggagaa ggccaaaacc gccctgaacg
     7021 cctacctgag gaacaccaag tggaatgtga tcatcaggga ggacctcctg agaattgaca
     7081 acaagacatg taccctgttc gcaaacaagg ccgtcgccct ggaagtggcc aggtatgtcc
     7141 acgcctatat caacgacatt gccgaggtca attcctactt ccaactgtac cattacatca
     7201 tgcagagaat tatcatgaat gagaggtacg agaaaagcag cggaaaggtg tccgagtact
     7261 tcgacgctgt gaatgacgag aagaagtaca acgataggct cctgaaactg ctgtgtgtgc
     7321 ctttcggcta ctgtatcccc aggtttaaga acctgagcat cgaggccctg ttcgatagga
     7381 acgaggccgc caagttcgac aaggagaaaa agaaggtgtc cggcaattcc accggtctgc
     7441 ctccacttga aagactgaca ctgggatccg gaggaggtgg aagcggtacc atggcggccg
     7501 ccagcggcta cacggacctg cgtgagaagc tcaagtccat gacgtcccgg gacaactata
     7561 aggcgggcag ccgggaggcc gccgccgctg ccgcagccgc cgtagccgcc gcagccgcag
     7621 ccgccgctgc cgccgaacct taccctgtgt ccggggccaa gcgcaagtat caggaggact
     7681 cggaccccga gcgcagcgac tatgaggagc agcagctgca gaaggaggag gaggcgcgca
     7741 aggtgaagag cggcatccgc cagatgcgcc tcttcagcca ggacgagtgc gccaagatcg
     7801 aggcccgcat tgacgaggtg gtgtcccgcg ctgagaaggg cctgtacaac gagcacacgg
     7861 tggaccgggc cccactgcgc aacaagtact tcttcggcga aggctacact tacggcgccc
     7921 agctgcagaa gcgcgggccc ggccaggagc gcctctaccc gccgggcgac gtggacgaga
     7981 tccccgagtg ggtgcaccag ctggtgatcc aaaagctggt ggagcaccgc gtcatccccg
     8041 agggcttcgt caacagcgcc gtcatcaacg actaccagcc cggcggctgc atcgtgtctc
     8101 acgtggaccc catccacatc ttcgagcgcc ccatcgtgtc cgtgtccttc tttagcgact
     8161 ctgcgctgtg cttcggctgc aagttccagt tcaagcctat tcgggtgtcg gaaccagtgc
     8221 tttccctgcc ggtgcgcagg ggaagcgtga ctgtgctcag tggatatgct gctgatgaaa
     8281 tcactcactg catacggcct caggacatca aggagcgccg agcagtcatc atcctcagga
     8341 agacaagatt agatgcaccc cggttggaaa caaagtccct gagcagctcc gtgttaccac
     8401 ccagctatgc ttcagatcgc ctgtcaggaa acaacaggga ccctgctctg aaacccaagc
     8461 ggtcccaccg caaggcagac cctgatgctg cccacaggcc acggatcctg gagatggaca
     8521 aggaagagaa ccggcgctcg gtgctgctgc ccacacaccg gcggaggggt agcttcagct
     8581 ctgagaacta ctggcgcaag tcatacgagt cctcagagga ctgctctgag gcagcaggca
     8641 gccctgcccg aaaggtgaag atgcggcggc actgagaatt cgcggccgct cgagtctaga
     8701 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8761 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8821 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8881 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8941 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     9001 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9061 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     9421 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     9481 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaacca
//
