LOCUS       V034853                 5916 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034853
VERSION     V034853
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(5884..5916,1..72))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     546..562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        589..892
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        893..1096
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          1234..1330
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             1394..1420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1448..3592
                     /label="ZDHHC5(NM_015457)"
                     /note="ZDHHC5(NM_015457)"
                     /gene="ZDHHC5"
     polyA_signal    3618..3752
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3862..3878)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3886..3902
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3910..3940)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3955..3976
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4264..4852)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5023..5883)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
       61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact
      601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
     1441 aattcccatg cccgcagagt ctggaaagag attcaaaccc agcaagtatg tcccggtctc
     1501 tgcagccgcc atcttcctag tgggagctac gacactcttc tttgccttta cgtgtccagg
     1561 actaagcctg tatgtgtcac ctgcagtgcc catctacaat gcaattatgt ttctctttgt
     1621 gttggccaac ttcagcatgg ccaccttcat ggacccaggg attttccctc gagctgagga
     1681 ggatgaggac aaggaagatg atttccgagc tcccctttac aaaacagtgg agataaaggg
     1741 catccaggtg cgcatgaaat ggtgtgccac ctgccgcttt taccgtcccc ctcgatgttc
     1801 ccactgcagt gtctgtgaca actgtgtgga ggaatttgat catcactgcc cctgggtgaa
     1861 taactgtatt ggtcgccgga actaccgtta ttttttcctt ttcctccttt ccctgacagc
     1921 ccacattatg ggtgtgtttg gctttggcct cctttatgtc ctctaccaca tagaggaact
     1981 ctcaggggtc cgcacggctg tcacaatggc agtaatgtgt gtggctggct tattcttcat
     2041 ccctgtagct ggcctcacgg gatttcacgt ggttctggtg gccaggggac gcacaaccaa
     2101 tgaacaggtt acgggtaaat tccggggagg tgtgaacccc ttcaccaatg gctgctgtaa
     2161 caatgtcagc cgtgttctct gcagttctcc agcacccagg tatttgggga gaccaaagaa
     2221 agagaagaca attgtaatca gacctccctt ccttcgacca gaagtttcag atgggcagat
     2281 aactgtgaag atcatggata atggcatcca gggagagctg aggagaacaa agtctaaggg
     2341 aagcctggag ataacagaga gccagtctgc agatgctgaa cctccacctc ctcctaagcc
     2401 agacctgagc cgttacacag ggttgcgaac acacctcggc ttggctacta atgaggatag
     2461 tagcttattg gccaaggaca gccccccgac acctaccatg tacaagtatc ggccgggtta
     2521 cagtagcagc agtacgtcag ctgccatgcc gcattcctcc agcgccaagt tgagtcgtgg
     2581 ggacagcttg aaggagccaa cctcaattgc agagagcagc cgtcacccca gctaccgctc
     2641 agagcccagc ttggaaccag agagcttccg ttctcctacc tttggcaaaa gttttcactt
     2701 cgatccacta tccagtggct cacgctcctc cagcctcaag tcagcccagg gcacaggctt
     2761 tgagctgggc cagttgcaat ccattcgttc agagggcacc acctccacct cctataagag
     2821 cctggccaac cagacacgca atggaagcct atcttatgac agcttgctca caccttcaga
     2881 cagccctgat tttgagtcag tgcaggcagg gcctgagcca gacccacctt taggctatac
     2941 ctctcccttc ctgtcagcca ggctggccca gcaacgggaa gctgagaggc acccacgttt
     3001 ggtgccaact ggcccaacac accgagagcc ctcaccagtc cgttacgaca atctgtcgcg
     3061 ccacattgtg gcctctctcc aggaacgaga gaagttgctg cgccagtcac ccccactccc
     3121 gggccgtgag gaagaaccag gcttggggga ctcaggcatt cagtcaacac caggctcggg
     3181 ccatgcccct cgtactagtt cctcctcaga tgattcaaag agatcacctt tgggcaagac
     3241 tccactggga cgcccagctg tcccccgttt tggcaagcca gatgggctaa ggggccgggg
     3301 agtagggtcc cctgaaccag gcccaacagc cccatacctg ggccgatcga tgtcttacag
     3361 cagccaaaaa gcccaacctg gtgtctctga gacagaagaa gtggccttgc agccattact
     3421 gacacccaaa gatgaagtac agctgaagac cacctacagc aaatccaacg ggcagcccaa
     3481 gagcttaggc tcagcctccc ctggcccagg ccagccacct ctcagtagcc ccacgagggg
     3541 aggagtcaag aaggtgtcag gggttggtgg taccacctat gagatttcgg tgtgactcga
     3601 ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     3661 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3721 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3781 ttcaggttca gggggaggtg tgggaggttt ttcggatcct ctagagtcga tctgcaggca
     3841 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3901 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3961 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4021 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4681 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5881 catactcttc ctttttcaat attattgaag cattta
//
