LOCUS       V030764                 6212 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030764
VERSION     V030764
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             55..2199
                     /label="ZDHHC5(NM_015457)"
                     /note="ZDHHC5(NM_015457)"
                     /gene="ZDHHC5"
     CDS             2257..2508
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    2523..2657
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2768..2784)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2792..2808
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2816..2846)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2861..2882
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3170..3758)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3929..4789)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4790..4894)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5368..5384
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        5411..5714
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        5715..5918
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          6056..6152
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattatgccc
       61 gcagagtctg gaaagagatt caaacccagc aagtatgtcc cggtctctgc agccgccatc
      121 ttcctagtgg gagctacgac actcttcttt gcctttacgt gtccaggact aagcctgtat
      181 gtgtcacctg cagtgcccat ctacaatgca attatgtttc tctttgtgtt ggccaacttc
      241 agcatggcca ccttcatgga cccagggatt ttccctcgag ctgaggagga tgaggacaag
      301 gaagatgatt tccgagctcc cctttacaaa acagtggaga taaagggcat ccaggtgcgc
      361 atgaaatggt gtgccacctg ccgcttttac cgtccccctc gatgttccca ctgcagtgtc
      421 tgtgacaact gtgtggagga atttgatcat cactgcccct gggtgaataa ctgtattggt
      481 cgccggaact accgttattt tttccttttc ctcctttccc tgacagccca cattatgggt
      541 gtgtttggct ttggcctcct ttatgtcctc taccacatag aggaactctc aggggtccgc
      601 acggctgtca caatggcagt aatgtgtgtg gctggcttat tcttcatccc tgtagctggc
      661 ctcacgggat ttcacgtggt tctggtggcc aggggacgca caaccaatga acaggttacg
      721 ggtaaattcc ggggaggtgt gaaccccttc accaatggct gctgtaacaa tgtcagccgt
      781 gttctctgca gttctccagc acccaggtat ttggggagac caaagaaaga gaagacaatt
      841 gtaatcagac ctcccttcct tcgaccagaa gtttcagatg ggcagataac tgtgaagatc
      901 atggataatg gcatccaggg agagctgagg agaacaaagt ctaagggaag cctggagata
      961 acagagagcc agtctgcaga tgctgaacct ccacctcctc ctaagccaga cctgagccgt
     1021 tacacagggt tgcgaacaca cctcggcttg gctactaatg aggatagtag cttattggcc
     1081 aaggacagcc ccccgacacc taccatgtac aagtatcggc cgggttacag tagcagcagt
     1141 acgtcagctg ccatgccgca ttcctccagc gccaagttga gtcgtgggga cagcttgaag
     1201 gagccaacct caattgcaga gagcagccgt caccccagct accgctcaga gcccagcttg
     1261 gaaccagaga gcttccgttc tcctaccttt ggcaaaagtt ttcacttcga tccactatcc
     1321 agtggctcac gctcctccag cctcaagtca gcccagggca caggctttga gctgggccag
     1381 ttgcaatcca ttcgttcaga gggcaccacc tccacctcct ataagagcct ggccaaccag
     1441 acacgcaatg gaagcctatc ttatgacagc ttgctcacac cttcagacag ccctgatttt
     1501 gagtcagtgc aggcagggcc tgagccagac ccacctttag gctatacctc tcccttcctg
     1561 tcagccaggc tggcccagca acgggaagct gagaggcacc cacgtttggt gccaactggc
     1621 ccaacacacc gagagccctc accagtccgt tacgacaatc tgtcgcgcca cattgtggcc
     1681 tctctccagg aacgagagaa gttgctgcgc cagtcacccc cactcccggg ccgtgaggaa
     1741 gaaccaggct tgggggactc aggcattcag tcaacaccag gctcgggcca tgcccctcgt
     1801 actagttcct cctcagatga ttcaaagaga tcacctttgg gcaagactcc actgggacgc
     1861 ccagctgtcc cccgttttgg caagccagat gggctaaggg gccggggagt agggtcccct
     1921 gaaccaggcc caacagcccc atacctgggc cgatcgatgt cttacagcag ccaaaaagcc
     1981 caacctggtg tctctgagac agaagaagtg gccttgcagc cattactgac acccaaagat
     2041 gaagtacagc tgaagaccac ctacagcaaa tccaacgggc agcccaagag cttaggctca
     2101 gcctcccctg gcccaggcca gccacctctc agtagcccca cgaggggagg agtcaagaag
     2161 gtgtcagggg ttggtggtac cacctatgag atttcggtgg gtacccgtcc ggcgtgcaaa
     2221 atcccgaacg acctgaaaca gaaagtcatg aaccacgaca agcagaagaa cggcatcaag
     2281 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     2341 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2401 taccagtcca aactgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2461 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg
     2521 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     2581 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     2641 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     2701 aggtgtggga ggttttttcg gatcctctag agtcgatctg caggcatgct agcttggcgt
     2761 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2941 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3001 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3061 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3601 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3901 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3961 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4021 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4081 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4141 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4201 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4261 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4321 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4381 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4441 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4501 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4561 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4621 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4681 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4741 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4921 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4981 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     5041 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     5101 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     5161 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     5221 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     5281 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     5341 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagttc gagcttgcat
     5401 gcctgcaggt cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     5461 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     5521 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     5581 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     5641 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     5701 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     5761 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     5821 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     5881 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     5941 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     6001 tccggactct agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag
     6061 tttagtcttt ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac
     6121 tgctcctcag tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct
     6181 aaaagctgcg gaattgtacc cgcgggccca cc
//
