LOCUS V034900 4623 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034900 VERSION V034900 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter complement(join(4591..4623,1..72)) /gene="bla" /label="AmpR promoter" primer_bind 546..562 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 589..892 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 893..1096 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 1234..1330 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" CDS 1394..1420 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1448..2299 /label="ZDHHC24(NM_207340)" /note="ZDHHC24(NM_207340)" /gene="ZDHHC24" polyA_signal 2325..2459 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(2569..2585) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 2593..2609 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(2617..2647) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2662..2683 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(2971..3559) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3730..4590) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" ORIGIN 1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact 601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg 1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt 1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt 1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct 1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg 1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg 1441 aattcccatg gggcagccct gggcggctgg gagcacggac ggggcgcccg cgcagctgcc 1501 tctcgtgctc accgcgctgt gggccgcggc cgtgggcctg gagctggctt acgtgctggt 1561 gctcggtccc gggccgccgc cgctgggacc cctggcccgg gccttgcagc tggcgctggc 1621 cgccttccag ctgctcaacc tgctgggcaa cgtggggctc ttcctgcgct cggatcccag 1681 catccgtggc gtgatgctgg ccggccgcgg tctgggccag ggctgggctt actgctacca 1741 atgccaaagc caggtgccgc cacgcagcgg acactgctct gcctgccgcg tctgcatcct 1801 gcgtcgggac caccactgcc gcctgctggg ccgctgcgtg ggcttcggca actaccggcc 1861 cttcctgtgc ctgctgcttc atgccgccgg cgtcctgctc cacgtctctg tgctgctggg 1921 ccctgcactg tcggccctgc tgcgagccca cacgcccctc cacatggctg ccctcctcct 1981 gcttccctgg ctcatgttgc tcacaggcag agtgtctctg gcacagtttg ccttggcctt 2041 cgtgacggac acgtgcgtgg cgggtgcgct gctgtgcggg gctgggctgc tcttccatgg 2101 gatgctgctg ctgcggggcc agaccacatg ggagtgggct cggggccagc actcctatga 2161 cctgggtccc tgccacaacc tgcaggcagc cctggggccc cgctgggccc tcgtctggct 2221 ctggcccttc ctggcctccc cattgcctgg ggatgggatc accttccaga ccacagcaga 2281 tgtgggacac acagcctcct gactcgaggt accgcggccg cggggatcca gacatgataa 2341 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 2401 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 2461 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttc 2521 ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt catagctgtt 2581 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 2641 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 2701 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 2761 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 2821 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 2941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 3001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 3061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 3121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 3181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 3241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 3301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 3361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 3421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 3481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 3541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 3601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 3661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 3721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 3781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 3841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 3901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 3961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 4021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 4081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 4141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 4201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 4261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 4321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 4381 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 4441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 4501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 4561 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 4621 tta //