LOCUS       V034921                 5076 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034921
VERSION     V034921
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(5044..5076,1..72))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     546..562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        589..892
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        893..1096
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          1234..1330
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             1394..1420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1448..2752
                     /label="ZDHHC23(NM_001320466)"
                     /note="ZDHHC23(NM_001320466)"
                     /gene="ZDHHC23"
     polyA_signal    2778..2912
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3022..3038)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3046..3062
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3070..3100)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3115..3136
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3424..4012)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4183..5043)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
       61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact
      601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
     1441 aattcccatg acacagaagg gcagtatgaa gcctgtgaag aaaaagaaaa ccgaagaacc
     1501 tgaattggag cccctgtgct gctgcgagta catagatcgg aatggggaaa agaaccacgt
     1561 ggctacttgt ttgtgtgatt gtcaagatct ggatgaaggg tgtgatcgat ggattacatg
     1621 taaatcttta cagccagaga cttgtgaaag aatcatggat acaatttctg atcgcctccg
     1681 aattccttgg cttaggggag ccaaaaaagt gaacatcagc atcatccctc cgcttgtcct
     1741 gctgcctgtc ttccttcatg tggcttcctg gcatttcctc ctgggggtgg tggttttgac
     1801 ctcccttcct gtgctggcac tgtggtacta ctacctcact cacagaagga aagaacagac
     1861 cctgtttttc ctgagccttg gactgttctc tctgggctac atgtactatg tgttcctgca
     1921 ggaagtggtc cccaaagggc gtgtgggtcc cgttcagctg gcggttctta cctgcgggtt
     1981 atttctgata ctcttagcct tgcacagagc caagaagaat ccaggctacc tcagcaatcc
     2041 agcaagcggt gacagatctc taagcagcag ccagctggag tgcctgagca gaaaagggca
     2101 ggagaagacc aaagggttcc ctggggcaga catgtcgggc agtctcaaca atcgcacaac
     2161 aaaggatgac cccaagggct cttccaagat gccagctgga agccccacca aagcgaagga
     2221 ggactggtgt gccaagtgcc agctggtgcg accagcccgg gcatggcact gccggatatg
     2281 tggcatctgt gtgaggagaa tggatcatca ttgtgtctgg ataaatagct gcgttggaga
     2341 atcaaatcat caagcattta tacttgccct tttgatcttc ttgctcacct cggtgtatgg
     2401 gatcacactg accttggaca ccatttgtag agacagaagt gtcttcacag ctcttttcta
     2461 ttgtcctgga gtttatgcaa attacagctc ggctctgtcc ttcacctgcg tgtggtactc
     2521 tgtgatcatc acagcaggca tggcctacat cttcctgatc cagctgatca acatcagcta
     2581 caatgtgact gagcgggaag tccagcaggc cctccgacag aagactgggc gccggctcct
     2641 ctgcgggctc atcgtggaca caggccagta caataggggc ttcctgcgga actggcacca
     2701 gttctccacc ctgggcacac gtgcattcca ccaccctgcc gaggacattg tctgactcga
     2761 ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     2821 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     2881 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     2941 ttcaggttca gggggaggtg tgggaggttt ttcggatcct ctagagtcga tctgcaggca
     3001 tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3841 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5041 catactcttc ctttttcaat attattgaag cattta
//
