LOCUS       V034919                 5667 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034919
VERSION     V034919
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(5635..5667,1..72))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     546..562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        589..892
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        893..1096
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          1234..1330
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             1394..1420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1448..3343
                     /label="ZDHHC17(NM_015336)"
                     /note="ZDHHC17(NM_015336)"
                     /gene="ZDHHC17"
     polyA_signal    3369..3503
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3613..3629)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3637..3653
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3661..3691)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3706..3727
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4015..4603)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4774..5634)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
       61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact
      601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
     1441 aattcccatg cagcgggagg agggatttaa caccaagatg gcggacggcc cggatgagta
     1501 cgataccgaa gcgggctgtg tgccccttct ccacccagag gaaatcaaac cccaaagcca
     1561 ttataaccat ggatatggtg aacctcttgg acggaaaact catattgatg attacagcac
     1621 atgggacata gtcaaggcta cacaatatgg aatatatgaa cgctgtcgag aattggtgga
     1681 agcaggttat gatgtacggc aaccggacaa agaaaatgtt accctcctcc attgggctgc
     1741 catcaataac agaatagatt tagtcaaata ctatatttcg aaaggtgcta ttgtggatca
     1801 acttggaggg gacctgaatt caactccatt gcactgggcc acaagacaag gccatctatc
     1861 catggttgtg caactaatga aatatggtgc agatccttca ttaattgatg gagaaggatg
     1921 tagctgtatt catctggctg ctcagttcgg acatacctca attgttgctt atctcatagc
     1981 aaaaggacag gatgtagata tgatggatca gaatggaatg acgcctttaa tgtgggcagc
     2041 atatagaaca catagtgtgg atccaactag attgctttta acattcaatg tttcagttaa
     2101 ccttggtgac aagtatcaca aaaacactgc tctgcattgg gcagtgctag cagggaatac
     2161 cacagtcatt agccttcttc tggaagctgg agctaatgtt gatgcccaga atatcaaggg
     2221 cgaatcagcg cttgatttgg caaaacagag aaaaaatgtg tggatgatca accacttaca
     2281 agaggcaagg caagcaaaag gatatgacaa tccgtccttc cttagaaagc tgaaagctga
     2341 taaggaattt cggcagaaag taatgttagg aactcctttc ctagttattt ggctggttgg
     2401 gtttatagca gacctaaata ttgattcttg gctcattaaa gggctaatgt atggtggtgt
     2461 ttgggctaca gtacagtttc tttcaaaatc ctttttcgat cattcaatgc atagtgcatt
     2521 gccccttggg atatatttgg caaccaaatt ctggatgtat gtgacgtggt tcttctggtt
     2581 ttggaatgat ctcaactttt tatttatcca tcttccattc cttgccaata gtgttgcact
     2641 tttctacaat tttggaaaat cttggaaatc agatccaggg attattaaag caacagaaga
     2701 gcaaaagaaa aagacaatag ttgaacttgc agagacagga agtctggacc tcagtatatt
     2761 ctgcagtacc tgtttgatac gaaaaccggt gaggtccaaa cattgtggtg tgtgcaaccg
     2821 ctgtatagca aaatttgatc atcattgccc atgggtgggt aactgtgtag gtgcaggcaa
     2881 ccatagatat tttatgggct acctattctt cttgcttttt atgatctgct ggatgattta
     2941 tggttgtata tcttactggg gactccactg tgagaccact tacaccaagg atggattttg
     3001 gacatacatt actcagattg ccacgtgttc accttggatg ttttggatgt tcctgaacag
     3061 tgttttccac ttcatgtggg tggctgtatt actcatgtgt cagatgtacc agatatcatg
     3121 tttaggtatt actacaaatg aaagaatgaa tgccaggaga tacaagcact ttaaagtcac
     3181 aacaacgtct attgaaagcc cattcaacca tggatgtgta agaaatatta tagacttctt
     3241 tgaatttcga tgctgtggcc tctttcgtcc tgttatcgtg gactggacca ggcagtatac
     3301 aatagaatat gaccaaatat caggatctgg gtaccagctg gtgtagctcg aggtaccgcg
     3361 gccgcgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3421 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3481 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3541 agggggaggt gtgggaggtt tttcggatcc tctagagtcg atctgcaggc atgctagctt
     3601 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3661 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3721 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4441 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4741 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4801 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4861 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4921 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4981 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5041 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5101 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5161 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5221 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5281 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5341 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5401 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5461 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5521 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5581 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5641 cctttttcaa tattattgaa gcattta
//
