LOCUS       V035057                 4782 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035057
VERSION     V035057
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(4750..4782,1..72))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     546..562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        589..892
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        893..1096
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          1234..1330
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             1394..1420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1448..2458
                     /label="ZDHHC15(NM_144969)"
                     /note="ZDHHC15(NM_144969)"
                     /gene="ZDHHC15"
     polyA_signal    2484..2618
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2728..2744)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2752..2768
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2776..2806)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2821..2842
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3130..3718)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3889..4749)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
       61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact
      601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
     1441 aattcccatg cggcgaggct ggaagatggc tctgtctggg gggctgcggt gctgccgccg
     1501 ggtactgtcc tgggtgccag tgctcgttat tgtcctcgtc gtgctctggt cctactatgc
     1561 ctacgtcttt gaactctgcc tggtgactgt tttgagccca gcagaaaaag ttatttacct
     1621 catactctac catgccatct ttgtgttctt tacctggacc tactggaagt ctatctttac
     1681 actcccacag cagccaaacc agaagttcca cttgtcctac acagacaagg agcgctatga
     1741 aaatgaagaa agacctgagg tccagaagca gatgcttgtt gatatggcca aaaagctacc
     1801 ggtttacaca agaactggaa gtggagctgt acgattctgt gaccggtgtc atctgatcaa
     1861 gccagaccgc tgccaccact gctctgtctg tgctatgtgt gtgttaaaaa tggatcatca
     1921 ctgcccttgg gttaataact gcattggatt ttccaactac aaattcttcc ttcaattctt
     1981 agcttactct gttctctact gcctgtacat tgctacgaca gtcttcagct atttcatcaa
     2041 atactggaga ggggaattac ccagtgttcg ctctaagttc catgtccttt ttcttctctt
     2101 tgtggcctgc atgttttttg tcagccttgt gattctcttt ggttaccatt gttggcttgt
     2161 cagcagaaac aaaaccacct tagaggcctt ctgcactcca gtgtttacaa gtggcccaga
     2221 gaaaaatggg ttcaaccttg gcttcatcaa gaatatccag caggtgtttg gagataagaa
     2281 gaagttctgg ttaataccta ttggttccag ccctggtgat ggacactcct tccctatgag
     2341 gtctatgaat gagtcacaga acccactgct agcaaatgaa gaaacctggg aagacaacga
     2401 ggatgacaac caagattatc cagaaggctc atcatctctt gctgtggaaa cggaaacata
     2461 gctcgaggta ccgcggccgc ggggatccag acatgataag atacattgat gagtttggac
     2521 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2581 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     2641 ttatgtttca ggttcagggg gaggtgtggg aggtttttcg gatcctctag agtcgatctg
     2701 caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3541 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4741 aatactcata ctcttccttt ttcaatatta ttgaagcatt ta
//
