LOCUS       V034206                 4525 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034206
VERSION     V034206
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             886..1638
                     /label="UNC119B(NM_001080533)"
                     /note="UNC119B(NM_001080533)"
                     /gene="UNC119B"
     polyA_signal    1664..1798
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1909..1925)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1933..1949
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1957..1987)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2002..2023
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2311..2899)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3070..3930)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3931..4035)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4509..4525
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcacatgag cgggtctaac
      901 ccgaaggctg cggccgcggc gtcggcggct gggcccgggg ggctggtggc tggcaaggag
      961 gagaagaaga aggcgggcgg cggcgtcctg aaccgcctga aggcgcggcg gcaggcgccc
     1021 caccacgcgg ccgacgacgg cgtcggggca gcggtcacgg agcaggagct gctggcgctg
     1081 gacaccatcc ggcccgagca cgtcctgcgc ctcagccggg tcaccgagaa ttatttatgt
     1141 aaacccgaag acaacatcta cagtattgat ttcacccgct tcaaaattcg agatttggag
     1201 acagggacag tactttttga gattgccaaa ccttgcgttt cagaccagga ggaggatgag
     1261 gaggagggag gtggagacgt ggacatcagc gcaggacgtt ttgtccgcta tcagttcaca
     1321 ccggcatttc tccgcctccg gacagtcggg gctacggtgg agttcacagt gggagacaaa
     1381 cctgtttcaa acttccggat gatcgaacgg cactatttcc gggaacactt gctgaaaaac
     1441 tttgactttg attttggctt ctgcatcccc agcagtagga acacttgtga acatatctat
     1501 gagtttcccc agctttcgga ggatgtcatt cgtctaatga ttgaaaatcc ttacgagacc
     1561 cgctctgaca gcttctactt tgttgacaac aagctgataa tgcacaacaa ggctgattat
     1621 gcctataatg gaggccagta actcgaggta ccgcggccgc ggggatccag acatgataag
     1681 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1741 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1801 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc
     1861 ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt catagctgtt
     1921 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1981 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     2101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4081 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     4141 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4201 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4261 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     4321 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     4381 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     4441 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     4501 acgacgttgt aaaacgacgg ccagt
//
