LOCUS       V028282                 5410 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028282
VERSION     V028282
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             841..870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             898..2523
                     /label="Traf3(NM_001364364)"
                     /note="Traf3(NM_001364364)"
                     /gene="Traf3"
     polyA_signal    2549..2683
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2794..2810)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2818..2834
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2842..2872)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2887..2908
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3196..3784)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3955..4815)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4816..4920)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5394..5410
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 gagcagaagc tgatctcaga ggaggacctg cttatggcca tggaggcccg aattcggatg
      901 gagtcaagca aaaagatgga tgctgctggc acactgcagc ctaacccacc cctaaagctg
      961 cagcctgatc gcggcgcagg gtccgtgctc gtgccggagc aaggaggcta caaggagaag
     1021 tttgtgaaga cggtggaaga caagtacaag tgcgagaagt gccgcctggt gctgtgcaac
     1081 ccgaagcaga cggagtgtgg ccaccggttc tgcgagagct gcatggccgc cctgctgagc
     1141 tcctccagtc caaaatgcac agcgtgccaa gaaagcatca tcaaagacaa ggtgtttaag
     1201 gataattgct gcaagagaga gattctggcc cttcaggtct actgtcggaa tgaaggcaga
     1261 ggttgtgcgg agcagctgac tctgggacat ctgctggtgc acctaaaaaa tgaatgtcag
     1321 tttgaggaac ttccctgtct gcgtgccgac tgcaaagaaa aagtactgag aaaagacttg
     1381 cgggatcacg tggaaaaggc ctgtaaatac cgcgaggcca cgtgcagtca ctgcaagagc
     1441 caagtgccca tgatcaaact gcagaaacat gaagacacag attgtccctg tgtggtggta
     1501 tcctgccctc acaagtgcag cgttcagact cttctaagga gtgagggtac aaaccagcag
     1561 atcaaggccc atgaggccag ctccgcggta cagcacgtga acctgctgaa ggagtggagc
     1621 aactccctgg agaagaaggt ttccctgctg cagaatgaaa gtgttgagaa aaacaagagc
     1681 atccaaagcc tgcacaacca gatctgcagc tttgagatcg agattgagag gcagaaggag
     1741 atgctccgaa acaacgagtc caagatcctt cacctgcagc gggtaatcga cagccaagca
     1801 gagaaactga aagaactgga caaggagatc cgtcccttcc ggcagaactg ggaggaagcg
     1861 gacagcatga agagcagtgt ggagtccctc cagaaccgag tgactgagct ggagagcgta
     1921 gacaaaagtg cggggcaggc ggctcgcaac acaggcttgc tggagtccca gctgagccgg
     1981 catgaccaga tgttgagtgt tcatgacatc cgcttggccg acatggacct gcggttccag
     2041 gtcctcgaga ccgccagcta caacggggtg ctgatctgga agatccgtga ctacaagcgc
     2101 cggaagcagg aggccgtcat ggggaagacc ctgtctctct acagccagcc tttctacaca
     2161 ggttattttg gctataagat gtgtgccagg gtctacctga atggggacgg aatggggaaa
     2221 gggacacact tgtcgctgtt ttttgtcatt atgcgtggag aatatgatgc tctgttgcca
     2281 tggccgttca agcagaaagt gacacttatg ctgatggatc aggggtcctc tcgccgtcat
     2341 ctgggagatg cgttcaagcc tgaccccaac agcagcagct tcaagaaacc caccggagag
     2401 atgaatatcg cctctggctg cccagtcttt gtcgcccaaa ctgttctaga gaacgggacg
     2461 tatattaaag atgatacaat ctttattaag gtcatagtgg atacctcgga tctgcctgac
     2521 ccctgactcg aggtaccgcg gccgcgggga tccagacatg ataagataca ttgatgagtt
     2581 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2641 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2701 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc ctctagagtc
     2761 gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3001 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4921 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4981 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5041 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5101 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5161 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     5221 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     5281 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     5341 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     5401 gacggccagt
//
