LOCUS       40924_11965        7380 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER31, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7380)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7380)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 7380)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7380)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    190..311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(318..773)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        800..904
                     /label=AmpR promoter
     promoter        906..1263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1298..2089
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2324..2371
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2700..3288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        3463..3766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3767..3970
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             4005..4721
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4728..4781
                     /label=2A
                     /note="2A"
     CDS             4728..4781
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4788..7325
                     /label=similar to TALER31
                     /note="similar to TALER31"
     CDS             7332..7352
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
ORIGIN
        1 agcttggtgg cggctcaact agttaagggc ccggcgcgcc taaggtaccc ccgggtctag
       61 agatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
      121 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
      181 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
      241 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
      301 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      361 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      421 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      481 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      541 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      601 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      661 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      721 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
      781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      961 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     1021 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1081 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     1141 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     1201 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     1261 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     1321 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     1381 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     1441 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     1501 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     1561 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     1621 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     1681 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     1741 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     1801 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     1861 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1921 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1981 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     2041 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     2101 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     2161 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     2221 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     2281 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     2341 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     2401 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     2461 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     2521 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     2581 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2821 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3361 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     3421 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3481 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3541 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3601 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3661 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3721 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3781 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3841 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3901 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3961 aagcagagct ggtttagtga accgtcagat ccgctagcga attcatggtg agcaagggcg
     4021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     4081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     4141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     4201 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     4261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4441 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     4501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     4621 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcaattggag ggccgcggca
     4741 gcctgctgac ctgcggcgac gtggaggaaa accccggccc caccggtatg tcgcggaccc
     4801 ggctcccttc cccacccgca cccagcccag cgttttcggc cgactcgttc tcagacctgc
     4861 ttaggcagtt cgacccctca ctgtttaaca catcgttgtt cgactccctt cctccgtttg
     4921 gggcgcacca tacggaggcg gccaccgggg agtgggatga ggtgcagtcg ggattgagag
     4981 ctgcggatgc accaccccca accatgcggg tggccgtcac cgctgcccga ccgccgaggg
     5041 cgaagcccgc accaaggcgg agggcagcgc aaccgtccga cgcaagcccc gcagcgcaag
     5101 tagatttgag aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga
     5161 ggtcgacagt cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca
     5221 tcgtagcctt gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca
     5281 tgattgcggc gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga
     5341 gcggagcccg agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc
     5401 ttcagctgga cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg
     5461 aggcggtgca cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg accccagagc
     5521 aggtcgtggc aatcgcctcc aacattggcg ggaaacaggc actcgagact gtccagcgcc
     5581 tgcttcccgt gctgtgccaa gcgcacggac tcaccccaga gcaggtcgtg gcaatcgcga
     5641 gcaataacgg cggaaaacag gctttggaaa cggtgcagag gctccttcca gtgctgtgcc
     5701 aagcgcacgg cctcacccca gagcaggtcg tggcaatcgc gagcaataac ggcggaaaac
     5761 aggctttgga aacggtgcag aggctccttc cagtgctgtg ccaagcgcac ggattaaccc
     5821 cagagcaggt cgtggcaatc gcctccaaca ttggcgggaa acaggcactc gagactgtcc
     5881 agcgcctgct tcccgtgctg tgccaagcgc acggcttaac cccagagcag gtcgtggcaa
     5941 tcgcgagcaa taacggcgga aaacaggctt tggaaacggt gcagaggctc cttccagtgc
     6001 tgtgccaagc gcacggactt accccagagc aggtcgtggc cattgcctcg aatggagggg
     6061 gcaaacaggc gttggaaacc gtacaacgat tgctgccggt gctgtgccaa gcgcacggcc
     6121 ttaccccaga gcaggtcgtg gcaatcgcga gcaataacgg cggaaaacag gctttggaaa
     6181 cggtgcagag gctccttcca gtgctgtgcc aagcgcacgg actaacccca gagcaggtcg
     6241 tggcaatcgc ctccaacatt ggcgggaaac aggcactcga gactgtccag cgcctgcttc
     6301 ccgtgctgtg ccaagcgcac gggctcaccc cagagcaggt cgtggcaatc gcgagcaata
     6361 acggcggaaa acaggctttg gaaacggtgc agaggctcct tccagtgctg tgccaagcgc
     6421 acgggctaac cccagagcag gtcgtggcca ttgcctcgaa tggagggggc aaacaggcgt
     6481 tggaaaccgt acaacgattg ctgccggtgc tgtgccaagc gcacggccta accccagagc
     6541 aggtcgtggc aatcgcctcc aacattggcg ggaaacaggc actcgagact gtccagcgcc
     6601 tgcttcccgt gctgtgccaa gcgcacgggt taaccccaga gcaggtcgtg gccattgcct
     6661 cgaatggagg gggcaaacag gcgttggaaa ccgtacaacg attgctgccg gtgctgtgcc
     6721 aagcgcacgg actcacgcct gagcaggtag tggctattgc atccaatatc gggggcagac
     6781 ccgcactgga gtcaatcgtg gcccagcttt cgaggccgga ccccgcgctg gccgcactca
     6841 ctaatgatca tcttgtagcg ctggcctgcc tcggcggacg acccgccttg gatgcggtga
     6901 agaaggggct cccgcacgcg cctgcattga ttaagcggac caacagaagg attcccgaga
     6961 ggacatcaca tcgagtggca gatcacgcgc aagtggtccg cgtgctcgga ttcttccagt
     7021 gtcactccca ccccgcacaa gcgttcgatg acgccatgac tcaatttggt atgtcgagac
     7081 acggactgct gcagctcttt cgtagagtcg gtgtcacaga actcgaggcc cgctcgggca
     7141 cactgcctcc cgcctcccag cggtgggaca ggattctcca agcgagcggt atgaaacgcg
     7201 cgaagccttc acctacgtca actcagacac ctgaccaggc gagccttcat gcgttcgcag
     7261 actcgctgga gagggatttg gacgcgccct cgcccatgca tgaaggggac caaactcgcg
     7321 cgtcagctag ccccaagaag aagagaaagg tggaggccag cccatggggc ggaggttcga
//
