LOCUS       40924_11950        7788 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER26, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 7788)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7788)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1319
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1326..1379
                     /label=2A
                     /note="2A"
     CDS             1326..1379
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    1386..4331
                     /label=similar to TALER26
                     /note="similar to TALER26"
     CDS             4338..4358
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4576..4697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4704..5159)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5186..5290
                     /label=AmpR promoter
     promoter        5292..5649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5684..6475
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6710..6757
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7086..7674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgaat
      601 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc
     1321 aattggaggg ccgcggcagc ctgctgacct gcggcgacgt ggaggaaaac cccggcccca
     1381 ccggtatgtc gcggacccgg ctcccttccc cacccgcacc cagcccagcg ttttcggccg
     1441 actcgttctc agacctgctt aggcagttcg acccctcact gtttaacaca tcgttgttcg
     1501 actcccttcc tccgtttggg gcgcaccata cggaggcggc caccggggag tgggatgagg
     1561 tgcagtcggg attgagagct gcggatgcac cacccccaac catgcgggtg gccgtcaccg
     1621 ctgcccgacc gccgagggcg aagcccgcac caaggcggag ggcagcgcaa ccgtccgacg
     1681 caagccccgc agcgcaagta gatttgagaa ctttgggata ttcacagcag cagcaggaaa
     1741 agatcaagcc caaagtgagg tcgacagtcg cgcagcatca cgaagcgctg gtgggtcatg
     1801 ggtttacaca tgcccacatc gtagccttgt cgcagcaccc tgcagccctt ggcacggtcg
     1861 ccgtcaagta ccaggacatg attgcggcgt tgccggaagc cacacatgag gcgatcgtcg
     1921 gtgtggggaa acagtggagc ggagcccgag cgcttgaggc cctgttgacg gtcgcgggag
     1981 agctgagagg gcctcccctt cagctggaca cgggccagtt gctgaagatc gcgaagcggg
     2041 gaggagtcac ggcggtcgag gcggtgcacg cgtggcgcaa tgcgctcacg ggagcacccc
     2101 tcaacctgac cccagagcag gtcgtggcaa ttgcgagcaa caacggggga aagcaggcac
     2161 tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc gcacggactt acgccagagc
     2221 aggtcgtggc aattgcgagc aacaacgggg gaaagcaggc actcgaaacc gtccagaggt
     2281 tgctgcctgt gctgtgccaa gcgcacggac taaccccaga gcaggtcgtg gcaattgcga
     2341 gcaacggagg gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc
     2401 aagcgcacgg gttgacccca gagcaggtcg tggcaattgc gagcaacatc gggggaaagc
     2461 aggcactcga aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggcctgaccc
     2521 cagagcaggt cgtggcaatt gcgagcaaca acgggggaaa gcaggcactc gaaaccgtcc
     2581 agaggttgct gcctgtgctg tgccaagcgc acggactgac accagagcag gtcgtggcaa
     2641 ttgcgagcaa catcggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc
     2701 tgtgccaagc gcacggactt acacccgaac aagtcgtggc aattgcgagc catgacgggg
     2761 gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggac
     2821 ttacgccaga gcaggtcgtg gcaattgcga gccatgacgg gggaaagcag gcactcgaaa
     2881 ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg actaacccca gagcaggtcg
     2941 tggcaattgc gagcaacgga gggggaaagc aggcactcga aaccgtccag aggttgctgc
     3001 ctgtgctgtg ccaagcgcac gggttgaccc cagagcaggt cgtggcaatt gcgagcaacg
     3061 gagggggaaa gcaggcactc gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc
     3121 acggcctgac cccagagcag gtcgtggcaa ttgcgagcaa catcggggga aagcaggcac
     3181 tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc gcacggactg acaccagagc
     3241 aggtcgtggc aattgcgagc aacaacgggg gaaagcaggc actcgaaacc gtccagaggt
     3301 tgctgcctgt gctgtgccaa gcgcacggcc tcaccccaga gcaggtcgtg gcaattgcga
     3361 gcaacaacgg gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc
     3421 aagcgcacgg acttacgcca gagcaggtcg tggcaattgc gagcaacaac gggggaaagc
     3481 aggcactcga aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggactaaccc
     3541 cagagcaggt cgtggcaatt gcgagccatg acgggggaaa gcaggcactc gaaaccgtcc
     3601 agaggttgct gcctgtgctg tgccaagcgc acgggttgac cccagagcag gtcgtggcaa
     3661 ttgcgagcaa cggaggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc
     3721 tgtgccaagc gcacggactc acgcctgagc aggtagtggc tattgcatcc aatatcgggg
     3781 gcagacccgc actggagtca atcgtggccc agctttcgag gccggacccc gcgctggccg
     3841 cactcactaa tgatcatctt gtagcgctgg cctgcctcgg cggacgaccc gccttggatg
     3901 cggtgaagaa ggggctcccg cacgcgcctg cattgattaa gcggaccaac agaaggattc
     3961 ccgagaggac atcacatcga gtggcagatc acgcgcaagt ggtccgcgtg ctcggattct
     4021 tccagtgtca ctcccacccc gcacaagcgt tcgatgacgc catgactcaa tttggtatgt
     4081 cgagacacgg actgctgcag ctctttcgta gagtcggtgt cacagaactc gaggcccgct
     4141 cgggcacact gcctcccgcc tcccagcggt gggacaggat tctccaagcg agcggtatga
     4201 aacgcgcgaa gccttcacct acgtcaactc agacacctga ccaggcgagc cttcatgcgt
     4261 tcgcagactc gctggagagg gatttggacg cgccctcgcc catgcatgaa ggggaccaaa
     4321 ctcgcgcgtc agctagcccc aagaagaaga gaaaggtgga ggccagccca tggggcggag
     4381 gttcgaagct tggtggcggc tcaactagtt aagggcccgg cgcgcctaag gtacccccgg
     4441 gtctagagat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     4501 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4561 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4681 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4741 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4801 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4861 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4921 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4981 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5041 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5101 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5281 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5341 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5401 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5461 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5521 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5581 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5641 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5701 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5761 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5821 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5881 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5941 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6001 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6061 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6121 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6181 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6241 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6301 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6361 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6421 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6481 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6541 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6601 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6661 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6721 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6781 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6841 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6901 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6961 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7021 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7081 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7141 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7201 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7261 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7321 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7381 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7441 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7501 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7561 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7621 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7681 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7741 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
