LOCUS       40924_11930        8184 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 8184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1319
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1326..1379
                     /label=2A
                     /note="2A"
     CDS             1326..1379
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    1395..4730
                     /label=similar to TALER21
                     /note="similar to TALER21"
     CDS             4740..4760
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4972..5093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5100..5555)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5582..5686
                     /label=AmpR promoter
     promoter        5688..6045
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6080..6871
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7106..7153
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7482..8070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgaat
      601 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc
     1321 aattggaggg ccgcggcagc ctgctgacct gcggcgacgt ggaggaaaac cccggcccca
     1381 ccggtttgtc gaccattcgt ccgcgcaggc caagtcctgc ccgcgagctt ctgcccggac
     1441 cccaaccgga tagggttcag ccgactgcag atcgtggggt gtctgcgcct gctggcagcc
     1501 ctctggatgg cttgcccgct cggcggacgg tgtcccggac ccggctgcca tctccccctg
     1561 cgccctcacc tgcgttctcg gcgggcagct tcagcgatct gctccgtccg ttcgatccgt
     1621 cgcttcttga tacatcgctt cttgattcga tgcctgccgt cggcacgccg catacagcgg
     1681 ctgccccagc agagtgggat gaggcgcaat cggctctgcg tgcagccgat gacccgccac
     1741 ccaccgtgcg tgtcgctgtc actgccgcgc ggccgccgcg cgccaagccg gccccgcgac
     1801 ggcgtgctgc gcaaccctcc gacgcttcgc cggccgcgca ggtggatcta cgcacgctcg
     1861 gctacagtca gcagcagcaa gagaagatca aaccgaaggt gcgttcgaca gtggcgcagc
     1921 accacgaggc actggtgggc catgggttta cacacgcgca catcgttgcg ctcagccaac
     1981 acccggcagc gttagggacc gtcgctgtca cgtatcagca cataatcacg gcgttgccag
     2041 aggcgacaca cgaagacatc gttggcgtcg gcaaacagtg gtccggcgca cgcgccctgg
     2101 aggccttgct cacggatgcg ggggagttga gaggtccgcc gttacagttg gacacaggcc
     2161 aacttgtgaa gattgcaaaa cgtggcggcg tgaccgcaat ggaggcagtg catgcatcgc
     2221 gcaatgcact gacgggtgcc cccctgaacc tgaccccgga ccaagtggtg gctatcgcca
     2281 gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     2341 aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc
     2401 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc
     2461 cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc
     2521 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     2581 tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     2641 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     2701 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     2761 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
     2821 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     2881 tggctatcgc cagcaacggt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2941 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg
     3001 gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     3061 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     3121 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     3181 aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc
     3241 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     3301 gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     3361 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     3421 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     3481 cggaccaagt ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc
     3541 agcggctgtt gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta
     3601 tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     3661 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     3721 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     3781 tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa
     3841 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     3901 tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     3961 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg
     4021 gtggcggcaa gcaagcgctc gaaagcattg tggcccagct gagccggcct gatccggcgt
     4081 tggccgcgtt gaccaacgac cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca
     4141 tggatgcagt gaaaaaggga ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc
     4201 gtattggcga acgcacgtcc catcgcgttg ccgactacgc gcaagtggtt cgcgtgctgg
     4261 agtttttcca gtgccactcc cacccagcgt acgcatttga tgaggccatg acgcagttcg
     4321 ggatgagcag gaacgggttg gtacagctct ttcgcagagt gggcgtcacc gaactcgaag
     4381 cccgcggtgg aacgctcccc ccagcctcgc agcgttggga ccgtatcctc caggcatcag
     4441 ggatgaaaag ggccaaaccg tcccctactt cagctcaaac accggatcag gcgtctttgc
     4501 atgcattcgc cgattcgctg gagcgtgacc ttgatgcgcc cagcccaatg cacgagggag
     4561 atcagacgcg ggcaagcagc cgtaaacggt cccgatcgga tcgtgctgtc accggcccct
     4621 ccgcacagca ggctgtcgag gtgcgcgttc ccgaacagcg cgatgcgctg catttgcccc
     4681 tcagctggag ggtaaaacgc ccgcgtacca ggatctgggg cggcctcccg gatcccagcc
     4741 ccaagaagaa gagaaaggtg gaggccagcg gtggcggctc aaagcttggt ggcggctcaa
     4801 ctagttaagg gcccggcgcg cctaaggtac ccccgggtct agagatccac cggatctaga
     4861 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     4921 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     4981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     5101 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     5161 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     5221 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     5281 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     5341 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     5401 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     5461 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     5521 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     5581 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5641 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     5701 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5761 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5821 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5881 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5941 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6001 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     6061 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     6121 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     6181 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     6241 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     6301 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     6361 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     6421 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     6481 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     6541 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6601 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     6661 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6721 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6781 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6841 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     6901 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     6961 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7021 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     7081 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     7141 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     7201 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     7261 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     7321 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7381 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7441 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7501 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7561 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7621 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7681 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7741 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7801 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7861 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7921 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7981 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8041 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8101 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8161 gataaccgta ttaccgccat gcat
//
