LOCUS       40924_11925       10217 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 10217)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10217)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     enhancer        93..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    631..655
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      664..673
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             673..714
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             727..747
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    757..3960
                     /label=similar to TALER20
                     /note="similar to TALER20"
     protein_bind    complement(4051..4075)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4230..4285
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4646..4662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4670..4686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4694..4724)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4739..4760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4933..5769
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(5799..5817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5838..5854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5862..5878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5886..5916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5931..5952)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6240..6828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7002..7859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7860..7964)
                     /label=AmpR promoter
     rep_origin      complement(7990..8445)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8587..8603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8613..8631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    8646..9449
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    9528..9553
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat tctagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 ggtttagtga accgtcagat cgaattcgac ccaagtttgt acaaaaaagc aggctgcggc
      661 cgcgccacca tgggaaaacc tattcctaat cctctgctgg gcctggattc taccggaggc
      721 atggccccta agaaaaagcg gaaggtggac ggcggagtgg acctgagaac actgggatat
      781 tctcagcagc agcaggagaa gatcaagccc aaggtgagat ccacagtggc ccagcaccac
      841 gaagccctgg tgggacacgg atttacacac gcccacattg tggccctgtc tcagcaccct
      901 gccgccctgg gaacagtggc cgtgaaatat caggatatga ttgccgccct gcctgaggcc
      961 acacacgaag ccattgtggg agtgggaaaa cagtggtctg gagccagagc cctggaagcc
     1021 ctgctgacag tggccggaga actgagagga cctcctctgc agctggatac aggacagctg
     1081 ctgaagattg ccaaaagggg cggagtgacc gcggtggaag ccgtgcacgc ctggagaaat
     1141 gccctgacag gagcccctct gaacctgacc cccgaacagg tggtggccat tgccagccac
     1201 gacggcggca agcaggccct ggaaaccgtg cagagactgc tgcccgtgct gtgccaggcc
     1261 catggcctga cacctgaaca ggtggtggct atcgcctcta acggcggagg aaaacaggct
     1321 ctggaaacag tgcagcggct gctgcctgtg ctgtgtcagg ctcacggctt gactccagaa
     1381 caggtggtgg ctattgcttc caatattggg gggaaacagg ccctggaaac tgtgcagcgc
     1441 ctgctgccag tgctgtgcca ggctcacgga ctgacccccg aacaggtggt ggccattgcc
     1501 agcaacaagg gcggcaagca ggccctggaa accgtgcaga gactgctgcc cgtgctgtgc
     1561 caggcccatg gcctgacacc tgaacaggtg gtggctatcg cctctaatat cggaggaaaa
     1621 caggctctgg aaacagtgca gcggctgctg cctgtgctgt gtcaggctca cggcttgact
     1681 ccagaacagg tggtggctat tgcttcccac gacgggggga aacaggccct ggaaactgtg
     1741 cagcgcctgc tgccagtgct gtgccaggct cacgggctga cccccgaaca ggtggtggcc
     1801 attgccagca acggcggcgg caagcaggcc ctggaaaccg tgcagagact gctgcccgtg
     1861 ctgtgccagg cccatggcct gacacctgaa caggtggtgg ctatcgcctc tcacgacgga
     1921 ggaaaacagg ctctggaaac agtgcagcgg ctgctgcctg tgctgtgtca ggctcacggc
     1981 ttgactccag aacaggtggt ggctattgct tccaacggcg gggggaaaca ggccctggaa
     2041 actgtgcagc gcctgctgcc agtgctgtgc caggctcacg gtttgacccc cgaacaggtg
     2101 gtggccattg ccagcaacgg cggcggcaag caggccctgg aaaccgtgca gagactgctg
     2161 cccgtgctgt gccaggccca tggcctgaca cctgaacagg tggtggctat cgcctctcac
     2221 gacggaggaa aacaggctct ggaaacagtg cagcggctgc tgcctgtgct gtgtcaggct
     2281 cacggcttga ctccagaaca ggtggtggct attgcttcca atattggggg gaaacaggcc
     2341 ctggaaactg tgcagcgcct gctgccagtg ctgtgccagg ctcacggcct cactcccgaa
     2401 caggtggtgg ccattgccag ccacgacggc ggcaagcagg ccctggaaac cgtgcagaga
     2461 ctgctgcccg tgctgtgcca ggcccatggc ctgacacctg aacaggtggt ggctatcgcc
     2521 tctaatatcg gaggaaaaca ggctctggaa acagtgcagc ggctgctgcc tgtgctgtgt
     2581 caggctcacg gcttgactcc agaacaggtg gtggctattg cttccaatat tggggggaaa
     2641 caggccctgg aaactgtgca gcgcctgctg ccagtgctgt gccaggctca cggactgacc
     2701 cccgaacagg tggtggccat tgccagcaac ggcggcggca agcaggccct ggaaaccgtg
     2761 cagagactgc tgcccgtgct gtgccaggcc catggcctga cacctgaaca ggtggtggct
     2821 atcgcctcta acaagggagg aaaacaggct ctggaaacag tgcagcggct gctgcctgtg
     2881 ctgtgtcagg ctcacggctt gactccagaa caggtggtgg ctattgcttc caacggcggg
     2941 gggaaacagg ccctggaaac tgtgcagcgc ctgctgccag tgctgtgcca ggctcacggg
     3001 ctgacccccg aacaggtggt ggccattgcc agcaacggcg gcggcaagca ggccctggaa
     3061 accgtgcaga gactgctgcc cgtgctgtgc caggcccatg gcctgacacc tgaacaggtg
     3121 gtggctatcg cctctcacga cggaggaaaa caggctctgg aaacagtgca gcggctgctg
     3181 cctgtgctgt gtcaggctca cggcttgact ccagaacagg tggtggctat tgcttccaac
     3241 ggcgggggga aacaggccct ggaaactgtg cagcgcctgc tgccagtgct gtgccaggct
     3301 cacggtttga cccccgaaca ggtggtggcc attgccagca acggcggcgg caagcaggcc
     3361 ctggaaaccg tgcagagact gctgcccgtg ctgtgccagg cccatggcct gacacctgaa
     3421 caggtggtgg ctatcgcctc taatatcgga ggaaaacagg ctctggaaac agtgcagcgg
     3481 ctgctgcctg tgctgtgtca ggctcacggc ttgactccac agcaggtcgt ggcaattgct
     3541 agcaacggcg gcggacggcc cgccctggag agcattgtgg cccagctgtc tagacctgat
     3601 cctgccctgg ccgccctgac aaatgatcac ctggtggccc tggcctgtct gggaggcaga
     3661 cctgccctgg atgccgtgaa aaaaggactg cctcacgccc ctgccctgat caagagaaca
     3721 aatagaagaa tccccgagcg gacctctcac agagtggccg atcacgccca ggtggtgaga
     3781 gtgctgggat tttttcagtg tcactctcac cctgcccagg cctttgatga tgccatgaca
     3841 cagtttggca tgagcagaca cggactgctg cagctgttta gaagagtggg agtgacagaa
     3901 ctggaggcca gatccggaac cctgcctcct gcctctcaga gatgggatag gattctgcag
     3961 ggttcccgtt taaacaagct tgtcgacggt accgaattca tcgatagtac tctcgaggga
     4021 tccgagctca agatcttagc taagtagacc acccagcttt cttgtacaaa gtggtacgcg
     4081 tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     4141 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     4201 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     4261 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     4321 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     4381 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     4441 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     4501 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     4561 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     4621 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4801 tgtcgtgcca gcggatcgac agtactaagc ttggtctaat acccaatctc tcgtcttatc
     4861 cagatgtttt atacgcctta attaataggg ataacagggt aatctcgagg tttaaacgtc
     4921 gacgcggccg ctactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc
     4981 cttcctagtc tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct
     5041 ggtgacacac ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc
     5101 ttacgatgga gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg
     5161 gggggatgcg tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc
     5221 tgagctgctc tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt
     5281 acacttccca agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt
     5341 tctaactttg gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt
     5401 tttacctgtg agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg
     5461 gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc
     5521 gtggccgcct ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg
     5581 ctatctggga catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg
     5641 ggcttctggg tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc
     5701 ttcctcgtcc accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg
     5761 ctctgctctc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     5821 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5881 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5941 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6001 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6661 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6901 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6961 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7021 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7081 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7141 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7201 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7261 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7321 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7381 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7441 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7501 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7561 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7621 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7681 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7741 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7801 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7861 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7921 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7981 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     8041 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     8101 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     8161 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     8221 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     8281 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8341 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     8401 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     8461 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8521 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8581 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     8641 gtacctgctt tctctgacca gcattctctc ccctgggcct gtgccgcttt ctgtctgcag
     8701 cttgtggcct gggtcacctc tacggctggc ccagatcctt ccctgccgcc tccttcaggt
     8761 tccgtcttcc tccactccct cttccccttg ctctctgctg tgttgctgcc caaggatgct
     8821 ctttccggag cacttccttc tcggcgctgc accacgtgat gtcctctgag cggatcctcc
     8881 ccgtgtctgg gtcctctccg ggcatctctc ctccctcacc caaccccatg ccgtcttcac
     8941 tcgctgggtt cccttttcct tctccttctg gggcctgtgc catctctcgt ttcttaggat
     9001 ggccttctcc gacggatgtc tcccttgcgt cccgcctccc cttcttgtag gcctgcatca
     9061 tcaccgtttt tctggacaac cccaaagtac cccgtctccc tggctttagc cacctctcca
     9121 tcctcttgct ttctttgcct ggacaccccg ttctcctgtg gattcgggtc acctctcact
     9181 cctttcattt gggcagctcc cctacccccc ttacctctct agtctgtgct agctcttcca
     9241 gccccctgtc atggcatctt ccaggggtcc gagagctcag ctagtcttct tcctccaacc
     9301 cgggccccta tgtccacttc aggacagcat gtttgctgcc tccagggatc ctgtgtcccc
     9361 gagctgggac caccttatat tcccagggcc ggttaatgtg gctctggttc tgggtacttt
     9421 tatctgtccc ctccacccca cagtggggca agcttattac cctgttatcc ctaaccggtg
     9481 cgtttttatg cttgtagtat tgtataatgt ttttaagatc caagcttctg acctcttctc
     9541 ttcctcccac agggcctcga gacaattgat taacatcgat acggtaccga gttggcgcgc
     9601 ctgggagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     9661 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
     9721 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
     9781 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
     9841 aaaagcttta ggctgaaaga gagatttaga atgacaggcg cgcctggcca tacatcgata
     9901 cggtaccgag ttggcgcgcc tgggagctca cggggacagc ccccccccaa agcccccagg
     9961 gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc
    10021 ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg
    10081 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac
    10141 acctgggggg atacggggaa aaagctttag gctgaaagag agatttagaa tgacaggcgc
    10201 gcctggtggc gaacgat
//
