LOCUS       40924_11900        8035 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER16, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 8035)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1319
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1326..1379
                     /label=2A
                     /note="2A"
     CDS             1326..1379
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    1394..4597
                     /label=similar to TALER16
                     /note="similar to TALER16"
     CDS             4608..4628
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4823..4944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4951..5406)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5433..5537
                     /label=AmpR promoter
     promoter        5539..5896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5931..6722
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6957..7004
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7333..7921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgaat
      601 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc
     1321 aattggaggg ccgcggcagc ctgctgacct gcggcgacgt ggaggaaaac cccggcccca
     1381 ccggtttgtc gacgtggacc tgagaacact gggatattct cagcagcagc aggagaagat
     1441 caagcccaag gtgagatcca cagtggccca gcaccacgaa gccctggtgg gacacggatt
     1501 tacacacgcc cacattgtgg ccctgtctca gcaccctgcc gccctgggaa cagtggccgt
     1561 gaaatatcag gatatgattg ccgccctgcc tgaggccaca cacgaagcca ttgtgggagt
     1621 gggaaaacag tggtctggag ccagagccct ggaagccctg ctgacagtgg ccggagaact
     1681 gagaggacct cctctgcagc tggatacagg acagctgctg aagattgcca aaaggggcgg
     1741 agtgaccgcg gtggaagccg tgcacgcctg gagaaatgcc ctgacaggag cccctctgaa
     1801 cctgaccccc gaacaggtgg tggccattgc cagcaacaag ggcggcaagc aggccctgga
     1861 aaccgtgcag agactgctgc ccgtgctgtg ccaggcccat ggcctgacac ctgaacaggt
     1921 ggtggctatc gcctctcacg acggaggaaa acaggctctg gaaacagtgc agcggctgct
     1981 gcctgtgctg tgtcaggctc acggcttgac tccagaacag gtggtggcta ttgcttccca
     2041 cgacgggggg aaacaggccc tggaaactgt gcagcgcctg ctgccagtgc tgtgccaggc
     2101 tcacggactg acccccgaac aggtggtggc cattgccagc cacgacggcg gcaagcaggc
     2161 cctggaaacc gtgcagagac tgctgcccgt gctgtgccag gcccatggcc tgacacctga
     2221 acaggtggtg gctatcgcct ctaacggcgg aggaaaacag gctctggaaa cagtgcagcg
     2281 gctgctgcct gtgctgtgtc aggctcacgg cttgactcca gaacaggtgg tggctattgc
     2341 ttcccacgac ggggggaaac aggccctgga aactgtgcag cgcctgctgc cagtgctgtg
     2401 ccaggctcac gggctgaccc ccgaacaggt ggtggccatt gccagcaaca agggcggcaa
     2461 gcaggccctg gaaaccgtgc agagactgct gcccgtgctg tgccaggccc atggcctgac
     2521 acctgaacag gtggtggcta tcgcctctaa caagggagga aaacaggctc tggaaacagt
     2581 gcagcggctg ctgcctgtgc tgtgtcaggc tcacggcttg actccagaac aggtggtggc
     2641 tattgcttcc cacgacgggg ggaaacaggc cctggaaact gtgcagcgcc tgctgccagt
     2701 gctgtgccag gctcacggtt tgacccccga acaggtggtg gccattgcca gccacgacgg
     2761 cggcaagcag gccctggaaa ccgtgcagag actgctgccc gtgctgtgcc aggcccatgg
     2821 cctgacacct gaacaggtgg tggctatcgc ctctaatatc ggaggaaaac aggctctgga
     2881 aacagtgcag cggctgctgc ctgtgctgtg tcaggctcac ggcttgactc cagaacaggt
     2941 ggtggctatt gcttccaacg gcggggggaa acaggccctg gaaactgtgc agcgcctgct
     3001 gccagtgctg tgccaggctc acggcctcac tcccgaacag gtggtggcca ttgccagcca
     3061 cgacggcggc aagcaggccc tggaaaccgt gcagagactg ctgcccgtgc tgtgccaggc
     3121 ccatggcctg acacctgaac aggtggtggc tatcgcctct aatatcggag gaaaacaggc
     3181 tctggaaaca gtgcagcggc tgctgcctgt gctgtgtcag gctcacggct tgactccaga
     3241 acaggtggtg gctattgctt ccaacaaggg ggggaaacag gccctggaaa ctgtgcagcg
     3301 cctgctgcca gtgctgtgcc aggctcacgg actgaccccc gaacaggtgg tggccattgc
     3361 cagcaacatc ggcggcaagc aggccctgga aaccgtgcag agactgctgc ccgtgctgtg
     3421 ccaggcccat ggcctgacac ctgaacaggt ggtggctatc gcctctaata tcggaggaaa
     3481 acaggctctg gaaacagtgc agcggctgct gcctgtgctg tgtcaggctc acggcttgac
     3541 tccagaacag gtggtggcta ttgcttccaa caaggggggg aaacaggccc tggaaactgt
     3601 gcagcgcctg ctgccagtgc tgtgccaggc tcacgggctg acccccgaac aggtggtggc
     3661 cattgccagc aacaagggcg gcaagcaggc cctggaaacc gtgcagagac tgctgcccgt
     3721 gctgtgccag gcccatggcc tgacacctga acaggtggtg gctatcgcct ctaatatcgg
     3781 aggaaaacag gctctggaaa cagtgcagcg gctgctgcct gtgctgtgtc aggctcacgg
     3841 cttgactcca gaacaggtgg tggctattgc ttccaacggc ggggggaaac aggccctgga
     3901 aactgtgcag cgcctgctgc cagtgctgtg ccaggctcac ggtttgaccc ccgaacaggt
     3961 ggtggccatt gccagccacg acggcggcaa gcaggccctg gaaaccgtgc agagactgct
     4021 gcccgtgctg tgccaggccc atggcctgac acctgaacag gtggtggcta tcgcctctaa
     4081 cggcggagga aaacaggctc tggaaacagt gcagcggctg ctgcctgtgc tgtgtcaggc
     4141 tcacggcttg actccacagc aggtcgtggc aattgctagc aacggcggcg gacggcccgc
     4201 cctggagagc attgtggccc agctgtctag acctgatcct gccctggccg ccctgacaaa
     4261 tgatcacctg gtggccctgg cctgtctggg aggcagacct gccctggatg ccgtgaaaaa
     4321 aggactgcct cacgcccctg ccctgatcaa gagaacaaat agaagaatcc ccgagcggac
     4381 ctctcacaga gtggccgatc acgcccaggt ggtgagagtg ctgggatttt ttcagtgtca
     4441 ctctcaccct gcccaggcct ttgatgatgc catgacacag tttggcatga gcagacacgg
     4501 actgctgcag ctgtttagaa gagtgggagt gacagaactg gaggccagat ccggaaccct
     4561 gcctcctgcc tctcagagat gggataggat tctgcaggga tcccagcccc aagaagaaga
     4621 gaaaggtgga ggccagcggt ggcggctcaa agcttggtgg cggctcaact agttaagggc
     4681 ccggcgcgcc taaggtaccc ccgggtctag ataactgatc ataatcagcc ataccacatt
     4741 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     4801 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     4861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4921 gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat
     4981 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     5041 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     5101 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     5161 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     5221 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     5281 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     5341 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     5401 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5461 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5521 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     5581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5641 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5701 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5761 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5821 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5881 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5941 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6001 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6061 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     6121 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6181 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6241 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6301 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6361 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6421 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     6481 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6541 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6601 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6661 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6721 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6781 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6841 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     6901 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     6961 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     7021 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     7081 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     7141 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     7201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7441 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
