LOCUS       40924_11885        7980 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER13, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 7980)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7980)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1319
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1326..1379
                     /label=2A
                     /note="2A"
     CDS             1326..1379
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    1395..4526
                     /label=similar to TALER13
                     /note="similar to TALER13"
     CDS             4536..4556
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4768..4889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4896..5351)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5378..5482
                     /label=AmpR promoter
     promoter        5484..5841
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5876..6667
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6902..6949
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7278..7866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgaat
      601 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc
     1321 aattggaggg ccgcggcagc ctgctgacct gcggcgacgt ggaggaaaac cccggcccca
     1381 ccggtttgtc gaccattcgt ccgcgcaggc caagtcctgc ccgcgagctt ctgcccggac
     1441 cccaaccgga tagggttcag ccgactgcag atcgtggggt gtctgcgcct gctggcagcc
     1501 ctctggatgg cttgcccgct cggcggacgg tgtcccggac ccggctgcca tctccccctg
     1561 cgccctcacc tgcgttctcg gcgggcagct tcagcgatct gctccgtccg ttcgatccgt
     1621 cgcttcttga tacatcgctt cttgattcga tgcctgccgt cggcacgccg catacagcgg
     1681 ctgccccagc agagtgggat gaggcgcaat cggctctgcg tgcagccgat gacccgccac
     1741 ccaccgtgcg tgtcgctgtc actgccgcgc ggccgccgcg cgccaagccg gccccgcgac
     1801 ggcgtgctgc gcaaccctcc gacgcttcgc cggccgcgca ggtggatcta cgcacgctcg
     1861 gctacagtca gcagcagcaa gagaagatca aaccgaaggt gcgttcgaca gtggcgcagc
     1921 accacgaggc actggtgggc catgggttta cacacgcgca catcgttgcg ctcagccaac
     1981 acccggcagc gttagggacc gtcgctgtca cgtatcagca cataatcacg gcgttgccag
     2041 aggcgacaca cgaagacatc gttggcgtcg gcaaacagtg gtccggcgca cgcgccctgg
     2101 aggccttgct cacggatgcg ggggagttga gaggtccgcc gttacagttg gacacaggcc
     2161 aacttgtgaa gattgcaaaa cgtggcggcg tgaccgcaat ggaggcagtg catgcatcgc
     2221 gcaatgcact gacgggtgcc cccctgaacc tgaccccgga ccaagtggtg gctatcgcca
     2281 gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     2341 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     2401 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgactc
     2461 cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc gaaacggtgc
     2521 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     2581 tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     2641 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacaatggcg
     2701 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     2761 tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa
     2821 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     2881 tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2941 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaaca
     3001 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     3061 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     3121 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc
     3181 aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg gtgcagcggc
     3241 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     3301 gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     3361 aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc
     3421 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     3481 cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc
     3541 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     3601 tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     3661 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
     3721 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     3781 tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa
     3841 gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc aacgaccacc
     3901 tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa aagggattgc
     3961 cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc acgtcccatc
     4021 gcgttgccga ctacgcgcaa gtggttcgcg tgctggagtt tttccagtgc cactcccacc
     4081 cagcgtacgc atttgatgag gccatgacgc agttcgggat gagcaggaac gggttggtac
     4141 agctctttcg cagagtgggc gtcaccgaac tcgaagcccg cggtggaacg ctccccccag
     4201 cctcgcagcg ttgggaccgt atcctccagg catcagggat gaaaagggcc aaaccgtccc
     4261 ctacttcagc tcaaacaccg gatcaggcgt ctttgcatgc attcgccgat tcgctggagc
     4321 gtgaccttga tgcgcccagc ccaatgcacg agggagatca gacgcgggca agcagccgta
     4381 aacggtcccg atcggatcgt gctgtcaccg gcccctccgc acagcaggct gtcgaggtgc
     4441 gcgttcccga acagcgcgat gcgctgcatt tgcccctcag ctggagggta aaacgcccgc
     4501 gtaccaggat ctggggcggc ctcccggatc ccagccccaa gaagaagaga aaggtggagg
     4561 ccagcggtgg cggctcaaag cttggtggcg gctcaactag ttaagggccc ggcgcgccta
     4621 aggtaccccc gggtctagag atccaccgga tctagataac tgatcataat cagccatacc
     4681 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4861 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     4921 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4981 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     5041 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     5101 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     5161 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     5221 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5281 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5341 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5401 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5461 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     5521 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5581 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5641 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5821 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     5881 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5941 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6001 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     6061 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6121 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6181 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6241 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6301 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6361 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     6421 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6481 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6541 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6601 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6661 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6721 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6781 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6841 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     6901 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     6961 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     7021 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     7081 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     7141 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7201 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7261 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7321 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7381 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7441 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7501 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7561 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7621 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7681 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7741 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7801 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7861 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7921 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
