LOCUS       40924_11880        8082 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCMV-TALER12, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8082)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 8082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1319
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1326..1379
                     /label=2A
                     /note="2A"
     CDS             1326..1379
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    1395..4628
                     /label=similar to TALER12
                     /note="similar to TALER12"
     CDS             4638..4658
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4870..4991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4998..5453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5480..5584
                     /label=AmpR promoter
     promoter        5586..5943
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5978..6769
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7004..7051
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7380..7968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgaat
      601 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      661 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      721 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      781 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      841 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      901 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      961 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1021 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1081 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1141 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1201 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1261 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc
     1321 aattggaggg ccgcggcagc ctgctgacct gcggcgacgt ggaggaaaac cccggcccca
     1381 ccggtttgtc gaccattcgt ccgcgcaggc caagtcctgc ccgcgagctt ctgcccggac
     1441 cccaaccgga tagggttcag ccgactgcag atcgtggggt gtctgcgcct gctggcagcc
     1501 ctctggatgg cttgcccgct cggcggacgg tgtcccggac ccggctgcca tctccccctg
     1561 cgccctcacc tgcgttctcg gcgggcagct tcagcgatct gctccgtccg ttcgatccgt
     1621 cgcttcttga tacatcgctt cttgattcga tgcctgccgt cggcacgccg catacagcgg
     1681 ctgccccagc agagtgggat gaggcgcaat cggctctgcg tgcagccgat gacccgccac
     1741 ccaccgtgcg tgtcgctgtc actgccgcgc ggccgccgcg cgccaagccg gccccgcgac
     1801 ggcgtgctgc gcaaccctcc gacgcttcgc cggccgcgca ggtggatcta cgcacgctcg
     1861 gctacagtca gcagcagcaa gagaagatca aaccgaaggt gcgttcgaca gtggcgcagc
     1921 accacgaggc actggtgggc catgggttta cacacgcgca catcgttgcg ctcagccaac
     1981 acccggcagc gttagggacc gtcgctgtca cgtatcagca cataatcacg gcgttgccag
     2041 aggcgacaca cgaagacatc gttggcgtcg gcaaacagtg gtccggcgca cgcgccctgg
     2101 aggccttgct cacggatgcg ggggagttga gaggtccgcc gttacagttg gacacaggcc
     2161 aacttgtgaa gattgcaaaa cgtggcggcg tgaccgcaat ggaggcagtg catgcatcgc
     2221 gcaatgcact gacgggtgcc cccctgaacc tgaccccgga ccaagtggtg gctatcgcca
     2281 gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     2341 aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc
     2401 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     2461 cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc
     2521 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     2581 tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     2641 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
     2701 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     2761 tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa
     2821 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     2881 tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     2941 cggtgctgtg ccaggaccat ggcctgactc cggaccaagt ggtggctatc gccagccacg
     3001 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     3061 atggcctgac cccggaccaa gtggtggcta tcgccagcaa caatggcggc aagcaagcgc
     3121 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg actccggacc
     3181 aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg gtgcagcggc
     3241 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgcca
     3301 gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     3361 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     3421 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     3481 cggaccaagt ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc
     3541 agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa gtggtggcta
     3601 tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     3661 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     3721 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     3781 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
     3841 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg
     3901 tggctatcgc cagcaacggt ggcggcaagc aagcgctcga aagcattgtg gcccagctga
     3961 gccggcctga tccggcgttg gccgcgttga ccaacgacca cctcgtcgcc ttggcctgcc
     4021 tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg ccggaattga
     4081 tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcc gactacgcgc
     4141 aagtggttcg cgtgctggag tttttccagt gccactccca cccagcgtac gcatttgatg
     4201 aggccatgac gcagttcggg atgagcagga acgggttggt acagctcttt cgcagagtgg
     4261 gcgtcaccga actcgaagcc cgcggtggaa cgctcccccc agcctcgcag cgttgggacc
     4321 gtatcctcca ggcatcaggg atgaaaaggg ccaaaccgtc ccctacttca gctcaaacac
     4381 cggatcaggc gtctttgcat gcattcgccg attcgctgga gcgtgacctt gatgcgccca
     4441 gcccaatgca cgagggagat cagacgcggg caagcagccg taaacggtcc cgatcggatc
     4501 gtgctgtcac cggcccctcc gcacagcagg ctgtcgaggt gcgcgttccc gaacagcgcg
     4561 atgcgctgca tttgcccctc agctggaggg taaaacgccc gcgtaccagg atctggggcg
     4621 gcctcccgga tcccagcccc aagaagaaga gaaaggtgga ggccagcggt ggcggctcaa
     4681 agcttggtgg cggctcaact agttaagggc ccggcgcgcc taaggtaccc ccgggtctag
     4741 agatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     4801 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4861 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4981 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     5041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     5101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     5161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     5221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     5281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     5461 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5521 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5581 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5641 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5701 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5761 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5821 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5881 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5941 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     6001 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6061 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     6121 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     6181 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6241 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6301 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6361 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6421 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6481 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     6541 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6601 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6661 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6721 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6781 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6841 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6901 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6961 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     7021 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     7081 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     7141 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     7201 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     7261 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7321 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7381 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7441 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7501 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7561 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7621 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7681 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7741 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7801 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7861 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7921 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7981 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8041 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
