LOCUS       .                       9290 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             908..4726
                     /gene="S"
                     /label="S"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus 2. Accession#: P0DTC2"
     CDS             4733..4822
                     /label="3xMyc"
                     /note="3 tandem Myc epitope tags"
     polyA_signal    4857..5081
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5127..5555
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5569..5898
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5965..6756
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6933..7066
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7103..7119)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7127..7143)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7151..7181)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7196..7217)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7505..8090)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8264..9121)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9122..9226)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tttgtttttc ttgttttatt gccactagtc tctagtcagt gtgttaatct
      961 tacaaccaga actcaattac cccctgcata cactaattct ttcacacgtg gtgtttatta
     1021 ccctgacaaa gttttcagat cctcagtttt acattcaact caggacttgt tcttaccttt
     1081 cttttccaat gttacttggt tccatgctat acatgtctct gggaccaatg gtactaagag
     1141 gtttgataac cctgtcctac catttaatga tggtgtttat tttgcttcca ctgagaagtc
     1201 taacataata agaggctgga tttttggtac tactttagat tcgaagaccc agtccctact
     1261 tattgttaat aacgctacta atgttgttat taaagtctgt gaatttcaat tttgtaatga
     1321 tccatttttg ggtgtttatt accacaaaaa caacaaaagt tggatggaaa gtgagttcag
     1381 agtttattct agtgcgaata attgcacttt tgaatatgtc tctcagcctt ttcttatgga
     1441 ccttgaagga aaacagggta atttcaaaaa tcttagggaa tttgtgttta agaatattga
     1501 tggttatttt aaaatatatt ctaagcacac gcctattaat ttagtgcgtg atctccctca
     1561 gggtttttcg gctttagaac cattggtaga tttgccaata ggtattaaca tcactaggtt
     1621 tcaaacttta cttgctttac atagaagtta tttgactcct ggtgattctt cttcaggttg
     1681 gacagctggt gctgcagctt attatgtggg ttatcttcaa cctaggactt ttctattaaa
     1741 atataatgaa aatggaacca ttacagatgc tgtagactgt gcacttgacc ctctctcaga
     1801 aacaaagtgt acgttgaaat ccttcactgt agaaaaagga atctatcaaa cttctaactt
     1861 tagagtccaa ccaacagaat ctattgttag atttcctaat attacaaact tgtgcccttt
     1921 tggtgaagtt tttaacgcca ccagatttgc atctgtttat gcttggaaca ggaagagaat
     1981 cagcaactgt gttgctgatt attctgtcct atataattcc gcatcatttt ccacttttaa
     2041 gtgttatgga gtgtctccta ctaaattaaa tgatctctgc tttactaatg tctatgcaga
     2101 ttcatttgta attagaggtg atgaagtcag acaaatcgct ccagggcaaa ctggaaagat
     2161 tgctgattat aattataaat taccagatga ttttacaggc tgcgttatag cttggaattc
     2221 taacaatctt gattctaagg ttggtggtaa ttataattac ctgtatagat tgtttaggaa
     2281 gtctaatctc aaaccttttg agagagatat ttcaactgaa atctatcagg ccggtagcac
     2341 accttgtaat ggtgttgaag gttttaattg ttactttcct ttacaatcat atggtttcca
     2401 acccactaat ggtgttggtt accaaccata cagagtagta gtactttctt ttgaacttct
     2461 acatgcacca gcaactgttt gtggacctaa aaagtctact aatttggtta aaaacaaatg
     2521 tgtcaatttc aacttcaatg gtttaacagg cacaggtgtt cttactgagt ctaacaaaaa
     2581 gtttctgcct ttccaacaat ttggcagaga cattgctgac actactgatg ctgtccgtga
     2641 tccacagaca cttgagattc ttgacattac accatgttct tttggtggtg tcagtgttat
     2701 aacaccagga acaaatactt ctaaccaggt tgctgttctt tatcaggatg ttaactgcac
     2761 agaagtccct gttgctattc atgcagatca acttactcct acttggcgtg tttattctac
     2821 aggttctaat gtttttcaaa cacgtgcagg ctgtttaata ggggctgaac atgtcaacaa
     2881 ctcatatgag tgtgacatac ccattggtgc aggtatatgc gctagttatc agactcagac
     2941 taattctcct cggcgggcac gtagtgtagc tagtcaatcc atcattgcct acactatgtc
     3001 acttggtgca gaaaattcag ttgcttactc taataactct attgccatac ccacaaattt
     3061 tactattagt gttaccacag aaattctacc agtgtctatg accaagacat cagtagattg
     3121 tacaatgtac atttgtggtg attcaactga atgcagcaat cttttgttgc aatatggcag
     3181 tttttgtaca caattaaacc gtgctttaac tggaatagct gttgaacaag acaaaaacac
     3241 ccaagaagtt tttgcacaag tcaaacaaat ttacaaaaca ccaccaatta aagattttgg
     3301 tggttttaat ttttcacaaa tattaccaga tccatcaaaa ccaagcaaga ggtcatttat
     3361 tgaagatcta cttttcaaca aagtgacact tgcagatgct ggcttcatca aacaatatgg
     3421 tgattgcctt ggtgatattg ctgctagaga cctcatttgt gcacaaaagt ttaacggcct
     3481 tactgttttg ccacctttgc tcacagatga aatgattgct caatacactt ctgcactgtt
     3541 agcgggtaca atcacttctg gttggacctt tggtgcaggt gctgcattac aaataccatt
     3601 tgctatgcaa atggcttata ggtttaatgg tattggagtt acacagaatg ttctctatga
     3661 gaaccaaaaa ttgattgcca accaatttaa tagtgctatt ggcaaaattc aagactcact
     3721 ttcttccaca gcaagtgcac ttggaaaact tcaagatgtg gtcaaccaaa atgcacaagc
     3781 tttaaacacg cttgttaaac aacttagctc caattttggt gcaatttcaa gtgttttaaa
     3841 tgatatcctt tcacgtcttg acaaagttga ggctgaagtg caaattgata ggttgatcac
     3901 aggcagactt caaagtttgc agacatatgt gactcaacaa ttaattagag ctgcagaaat
     3961 cagagcttct gctaatcttg ctgctactaa aatgtcagag tgtgtacttg gacaatcaaa
     4021 aagagttgat ttttgtggaa agggctatca tcttatgtcc ttccctcagt cagcacctca
     4081 tggtgtagtc ttcttgcatg tgacttatgt ccctgcacaa gaaaagaact tcacaactgc
     4141 tcctgccatt tgtcatgatg gaaaagcaca ctttcctcgt gaaggtgtct ttgtttcaaa
     4201 tggcacacac tggtttgtaa cacaaaggaa tttttatgaa ccacaaatca ttactacaga
     4261 caacacattt gtgtctggta actgtgatgt tgtaatagga attgtcaaca acacagttta
     4321 tgatcctttg caacctgaat tagactcatt caaggaggag ttagataaat attttaagaa
     4381 tcatacatca ccagatgttg atttaggtga catctctggc attaatgctt cagttgtaaa
     4441 cattcaaaaa gaaattgacc gcctcaatga ggttgccaag aatttaaatg aatctctcat
     4501 cgatctccaa gaacttggaa agtatgagca gtatataaaa tggccatggt acatttggct
     4561 aggttttata gctggcttga ttgccatagt aatggtgaca attatgcttt gctgtatgac
     4621 cagttgctgt agttgtctca agggctgttg ttcttgtgga tcctgctgca aatttgatga
     4681 agacgactct gagccagtgc tcaaaggagt caaattacat tacacactcg aggaacaaaa
     4741 actaatatca gaggaagatt tggaacagaa actaatcagt gaagaagatt tagagcaaaa
     4801 gttaatttcc gaggaggact tatgagggcc cgtttaaacc cgctgatcag cctcgactgt
     4861 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4921 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4981 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5041 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5101 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5281 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5341 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5461 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5521 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5581 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5641 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5701 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5761 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5821 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5881 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5941 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6001 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6061 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6121 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6181 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6241 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6301 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6361 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6421 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6481 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6541 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6601 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6661 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6721 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6781 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6841 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6901 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7021 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7081 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7141 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7201 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7261 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7321 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7381 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7441 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7501 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7561 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7621 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7681 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7741 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7801 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7861 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7921 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7981 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9241 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
