LOCUS       V021575                 5006 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021575
VERSION     V021575
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             58..987
                     /label="Sirt5(NM_178848)"
                     /note="Sirt5(NM_178848)"
                     /gene="Sirt5"
     CDS             1051..1302
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    1317..1451
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1562..1578)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1586..1602
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1610..1640)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1655..1676
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(1964..2552)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2723..3583)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3584..3688)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4162..4178
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        4205..4508
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        4509..4712
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          4850..4946
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattctcatg
       61 cgacctctcc tgattgctcc gggccgattc atttcccagt tgtgttgtag acgaaagcct
      121 cctgcctccc cacaaagcaa gatctgcctc accatggctc gtccaagttc aaatatggca
      181 gactttcgga agtgttttgc gaacgccaag cacatagcca tcatctcggg ggctggcgtt
      241 agtgcggaga gtggggttcc cactttcaga ggcgctggag gttactggag aaaatggcag
      301 gctcaggacc tggcaacccc tcaggccttt gctcgaaacc catcacaggt gtgggagttt
      361 taccactacc ggagggaggt catgcggagc aaagaaccca accccgggca cctggccatt
      421 gcccagtgtg aagcccggct tcgtgaccag ggcagacggg ttgtggtcat cacccagaac
      481 attgacgagt tgcatcgcaa ggctggcacc aagaaccttc tggaaatcca cggaacctta
      541 tttaaaactc ggtgtacctc gtgtggcact gttgccgaga actataggag tccgatctgc
      601 ccagctttag caggaaaagg ggccccagag ccagagactc aagacgccag aatcccagtc
      661 gacaaacttc cccggtgcga ggaggcagga tgcggaggct tgctgcgacc tcacgtggtg
      721 tggtttggag aaaacctgga tcctgccatt ctggaggagg tggacagaga gctcgccctc
      781 tgtgacctgt gtctagtggt gggaacatcc tctgtggtct acccggctgc catgtttgcc
      841 cctcaggtgg cttccagggg agtcccggtg gccgagttta acatggagac caccccagcc
      901 accgacagat tcaggtttca ttttcccgga ccctgtggga aaactcttcc tgaagccctt
      961 gctcctcatg aaactgaaag gacttctact cgaggtaccc gtccggcgtg caaaatcccg
     1021 aacgacctga aacagaaagt catgaaccac gacaagcaga agaacggcat caaggccaac
     1081 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     1141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1201 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1261 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagcggc cgcggggatc
     1321 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     1381 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     1441 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     1501 gggaggtttt ttcggatcct ctagagtcga tctgcaggca tgctagcttg gcgtaatcat
     1561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2401 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     3721 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     3781 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3841 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3901 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     3961 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4021 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     4081 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     4141 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gttcgagctt gcatgcctgc
     4201 aggtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     4261 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     4321 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     4381 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4441 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4501 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4561 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4621 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4681 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4741 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     4801 ctctagagga tccggtacta gaggaactga aaaaccagaa agttaactgg taagtttagt
     4861 ctttttgtct tttatttcag gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc
     4921 tcagtggatg ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc
     4981 tgcggaattg tacccgcggg cccacc
//
