LOCUS       40924_11755        4278 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCMV-Script, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Hosfield T, Padgett K, Sanchez T, Lu Q.
  TITLE     Mammalian expression vector for efficient cloning of PCR fragments
  JOURNAL   Strategies 10, 68-69 (1997)
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Hosfield T, Padgett K, Sanchez T, Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-1997) Marketing Analysis, Stratagene, 11011 North 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 4278)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Strategies 
            10, 68-69 (1997)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-1997) Marketing Analysis, Stratagene, 11011 North Torrey 
            Pines Rd, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..758
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(780..798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1072..1193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1200..1655)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1682..1784
                     /label=AmpR promoter
     promoter        1788..2145
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2180..2971
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3206..3253
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3582..4170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgctctagc ccgggcggat cccccgggct gcaggaattc gatatcaagc
      721 ttatcgatac cgtcgacctc gagggggggc ccggtaccag gtaagtgtac ccaattcgcc
      781 ctatagtgag tcgtattaca attcactcga tcgcccttcc caacagttgc gcagcctgaa
      841 tggcgaatgg agatccaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
      901 tgtgtatttt agattcacag tcccaaggct catttcaggc ccctcagtcc tcacagtctg
      961 ttcatgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     1021 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     1081 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1141 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     1201 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     1261 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     1321 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     1381 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     1441 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     1501 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     1561 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1621 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     1681 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1741 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagaatcctg aggcggaaag
     1801 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     1861 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     1921 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     1981 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2041 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2101 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     2161 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     2221 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     2281 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     2341 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     2401 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     2461 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     2521 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     2581 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     2641 tcaggatgat ctggacgaag aacatcaggg gctcgcgcca gccgaactgt tcgccaggct
     2701 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     2761 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     2821 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag aacttggcgg
     2881 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     2941 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     3001 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     3061 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     3121 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     3181 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     3241 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     3301 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     3361 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     3421 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3721 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4261 gataaccgta ttaccgcc
//
