LOCUS       V037554                10222 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037554
VERSION     V037554
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             937..5973
                     /label="SMARCA4(NM_001128849)"
                     /note="SMARCA4(NM_001128849)"
                     /gene="SMARCA4"
     CDS             5986..6051
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    6086..6310
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6356..6784
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6798..7127
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7139..7738
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    7898..8019
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8068..8084)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8092..8108
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8116..8146)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8161..8182
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8470..9055)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9226..10086)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10087..10191)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcga tatcggatcc gccaccatgt ccactccaga cccacccctg
      961 ggcggaactc ctcggccagg tccttccccg ggccctggcc cttcccctgg agccatgctg
     1021 ggccctagcc cgggtccctc gccgggctcc gcccacagca tgatggggcc cagcccaggg
     1081 ccgccctcag caggacaccc catccccacc caggggcctg gagggtaccc tcaggacaac
     1141 atgcaccaga tgcacaagcc catggagtcc atgcatgaga agggcatgtc ggacgacccg
     1201 cgctacaacc agatgaaagg aatggggatg cggtcagggg gccatgctgg gatggggccc
     1261 ccgcccagcc ccatggacca gcactcccaa ggttacccct cgcccctggg tggctctgag
     1321 catgcctcta gtccagttcc agccagtggc ccgtcttcgg ggccccagat gtcttccggg
     1381 ccaggaggtg ccccgctgga tggtgctgac ccccaggcct tggggcagca gaaccggggc
     1441 ccaaccccat ttaaccagaa ccagctgcac cagctcagag ctcagatcat ggcctacaag
     1501 atgctggcca gggggcagcc cctccccgac cacctgcaga tggcggtgca gggcaagcgg
     1561 ccgatgcccg ggatgcagca gcagatgcca acgctacctc caccctcggt gtccgcaaca
     1621 ggacccggcc ctggccctgg ccctggcccc ggcccgggtc ccggcccggc acctccaaat
     1681 tacagcaggc ctcatggtat gggagggccc aacatgcctc ccccaggacc ctcgggcgtg
     1741 ccccccggga tgccaggcca gcctcctgga gggcctccca agccctggcc tgaaggaccc
     1801 atggcgaatg ctgctgcccc cacgagcacc cctcagaagc tgattccccc gcagccaacg
     1861 ggccgccctt cccccgcgcc ccctgccgtc ccacccgccg cctcgcccgt gatgccaccg
     1921 cagacccagt cccccgggca gccggcccag cccgcgccca tggtgccact gcaccagaag
     1981 cagagccgca tcacccccat ccagaagccg cggggcctcg accctgtgga gatcctgcag
     2041 gagcgcgagt acaggctgca ggctcgcatc gcacaccgaa ttcaggaact tgaaaacctt
     2101 cccgggtccc tggccgggga tttgcgaacc aaagcgacca ttgagctcaa ggccctcagg
     2161 ctgctgaact tccagaggca gctgcgccag gaggtggtgg tgtgcatgcg gagggacaca
     2221 gcgctggaga cagccctcaa tgctaaggcc tacaagcgca gcaagcgcca gtccctgcgc
     2281 gaggcccgca tcactgagaa gctggagaag cagcagaaga tcgagcagga gcgcaagcgc
     2341 cggcagaagc accaggaata cctcaatagc attctccagc atgccaagga tttcaaggaa
     2401 tatcacagat ccgtcacagg caaaatccag aagctgacca aggcagtggc cacgtaccat
     2461 gccaacacgg agcgggagca gaagaaagag aacgagcgga tcgagaagga gcgcatgcgg
     2521 aggctcatgg ctgaagatga ggaggggtac cgcaagctca tcgaccagaa gaaggacaag
     2581 cgcctggcct acctcttgca gcagacagac gagtacgtgg ctaacctcac ggagctggtg
     2641 cggcagcaca aggctgccca ggtcgccaag gagaaaaaga agaaaaagaa aaagaagaag
     2701 gcagaaaatg cagaaggaca gacgcctgcc attgggccgg atggcgagcc tctggacgag
     2761 accagccaga tgagcgacct cccggtgaag gtgatccacg tggagagtgg gaagatcctc
     2821 acaggcacag atgcccccaa agccgggcag ctggaggcct ggctcgagat gaacccgggg
     2881 tatgaagtag ctccgaggtc tgatagtgaa gaaagtggct cagaagaaga ggaagaggag
     2941 gaggaggaag agcagccgca ggcagcacag cctcccaccc tgcccgtgga ggagaagaag
     3001 aagattccag atccagacag cgatgacgtc tctgaggtgg acgcgcggca catcattgag
     3061 aatgccaagc aagatgtcga tgatgaatat ggcgtgtccc aggcccttgc acgtggcctg
     3121 cagtcctact atgccgtggc ccatgctgtc actgagagag tggacaagca gtcagcgctt
     3181 atggtcaatg gtgtcctcaa acagtaccag atcaaaggtt tggagtggct ggtgtccctg
     3241 tacaacaaca acctgaacgg catcctggcc gacgagatgg gcctggggaa gaccatccag
     3301 accatcgcgc tcatcacgta cctcatggag cacaaacgca tcaatgggcc cttcctcatc
     3361 atcgtgcctc tctcaacgct gtccaactgg gcgtacgagt ttgacaagtg ggccccctcc
     3421 gtggtgaagg tgtcttacaa gggatcccca gcagcaagac gggcctttgt cccccagctc
     3481 cggagtggga agttcaacgt cttgctgacg acgtacgagt acatcatcaa agacaagcac
     3541 atcctcgcca agatccgttg gaagtacatg attgtggacg aaggtcaccg catgaagaac
     3601 caccactgca agctgacgca ggtgctcaac acgcactatg tggcaccccg ccgcctgctg
     3661 ctgacgggca caccgctgca gaacaagctt cccgagctct gggcgctgct caacttcctg
     3721 ctgcccacca tcttcaagag ctgcagcacc ttcgagcagt ggtttaacgc accctttgcc
     3781 atgaccgggg aaaaggtgga cctgaatgag gaggaaacca ttctcatcat ccggcgtctc
     3841 cacaaagtgc tgcggccctt cttgctccga cgactcaaga aggaagtcga ggcccagttg
     3901 cccgaaaagg tggagtacgt catcaagtgc gacatgtctg cgctgcagcg agtgctctac
     3961 cgccacatgc aggccaaggg cgtgctgctg actgatggct ccgagaagga caagaagggc
     4021 aaaggcggca ccaagaccct gatgaacacc atcatgcagc tgcggaagat ctgcaaccac
     4081 ccctacatgt tccagcacat cgaggagtcc ttttccgagc acttggggtt cactggcggc
     4141 attgtccaag ggctggacct gtaccgagcc tcgggtaaat ttgagcttct tgatagaatt
     4201 cttcccaaac tccgagcaac caaccacaaa gtgctgctgt tctgccaaat gacctccctc
     4261 atgaccatca tggaagatta ctttgcgtat cgcggcttta aatacctcag gcttgatgga
     4321 accacgaagg cggaggaccg gggcatgctg ctgaaaacct tcaacgagcc cggctctgag
     4381 tacttcatct tcctgctcag cacccgggct ggggggctcg gcctgaacct ccagtcggca
     4441 gacactgtga tcatttttga cagcgactgg aatcctcacc aggacctgca agcgcaggac
     4501 cgagcccacc gcatcgggca gcagaacgag gtgcgtgtgc tccgcctctg caccgtcaac
     4561 agcgtggagg agaagatcct agctgcagcc aagtacaagc tcaacgtgga ccagaaggtg
     4621 atccaggccg gcatgttcga ccagaagtcc tccagccatg agcggcgcgc cttcctgcag
     4681 gccatcctgg agcacgagga gcaggatgag agcagacact gcagcacggg cagcggcagt
     4741 gccagcttcg cccacactgc ccctccgcca gcgggcgtca accccgactt ggaggagcca
     4801 cctctaaagg aggaagacga ggtgcccgac gacgagaccg tcaaccagat gatcgcccgg
     4861 cacgaggagg agtttgatct gttcatgcgc atggacctgg accgcaggcg cgaggaggcc
     4921 cgcaacccca agcggaagcc gcgcctcatg gaggaggacg agctcccctc gtggatcatc
     4981 aaggacgacg cggaggtgga gcggctgacc tgtgaggagg aggaggagaa gatgttcggc
     5041 cgtggctccc gccaccgcaa ggaggtggac tacagcgact cactgacgga gaagcagtgg
     5101 ctcaagaaaa ttacaggaaa agatatccat gacacagcca gcagtgtggc acgtgggcta
     5161 caattccagc gtggccttca gttctgcaca cgtgcgtcaa aggccatcga ggagggcacg
     5221 ctggaggaga tcgaagagga ggtccggcag aagaaatcat cacggaagcg caagcgagac
     5281 agcgacgccg gctcctccac cccgaccacc agcacccgca gccgcgacaa ggacgacgag
     5341 agcaagaagc agaagaagcg cgggcggccg cctgccgaga aactctcccc taacccaccc
     5401 aacctcacca agaagatgaa gaagattgtg gatgccgtga tcaagtacaa ggacagcagc
     5461 agtggacgtc agctcagcga ggtcttcatc cagctgccct cgcgaaagga gctgcccgag
     5521 tactacgagc tcatccgcaa gcccgtggac ttcaagaaga taaaggagcg cattcgcaac
     5581 cacaagtacc gcagcctcaa cgacctagag aaggacgtca tgctcctgtg ccagaacgca
     5641 cagaccttca acctggaggg ctccctgatc tatgaagact ccatcgtctt gcagtcggtc
     5701 ttcaccagcg tgcggcagaa aatcgagaag gaggatgaca gtgaaggcga ggagagtgag
     5761 gaggaggaag agggcgagga ggaaggctcc gaatccgaat ctcggtccgt caaagtgaag
     5821 atcaagcttg gccggaagga gaaggcacag gaccggctga agggcggccg gcggcggccg
     5881 agccgagggt cccgagccaa gccggtcgtg agtgacgatg acagtgagga ggaacaagag
     5941 gaggaccgct caggaagtgg cagcgaagaa gacctcgaga ccggtgacta caaagaccat
     6001 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gtgaccggta
     6061 agtttaaacc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6121 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6181 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6241 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6301 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6361 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6421 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6481 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6541 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6601 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6661 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6721 attttggcca tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6781 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6841 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6901 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6961 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7021 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7081 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggat
     7141 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     7201 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     7261 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7321 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7381 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     7441 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7501 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7561 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     7621 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7681 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatt
     7741 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7801 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7861 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8041 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8101 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8161 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8221 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8281 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8341 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8401 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8461 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8521 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8581 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8641 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8701 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8761 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8821 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8881 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8941 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9001 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10201 ccgaaaagtg ccacctgacg tc
//
