LOCUS       V029905                12766 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029905
VERSION     V029905
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             937..8517
                     /label="LRRK2(NM_198578)"
                     /note="LRRK2(NM_198578)"
                     /gene="LRRK2"
     CDS             8530..8595
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    8630..8854
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      8900..9328
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        9342..9671
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9683..10282
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    10442..10563
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10612..10628)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10636..10652
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10660..10690)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10705..10726
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11014..11599)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11770..12630)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12631..12735)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcga tatcggatcc gccaccatgg ctagtggcag ctgtcagggg
      961 tgcgaagagg acgaggaaac tctgaagaag ttgatagtca ggctgaacaa tgtccaggaa
     1021 ggaaaacaga tagaaacgct ggtccaaatc ctggaggatc tgctggtgtt cacgtactcc
     1081 gagcgcgcct ccaagttatt tcaaggcaaa aatatccatg tgcctctgtt gatcgtcttg
     1141 gactcctata tgagagtcgc gagtgtgcag caggtgggtt ggtcacttct gtgcaaatta
     1201 atagaagtct gtccaggtac aatgcaaagc ttaatgggac cccaggatgt tggaaatgat
     1261 tgggaagtcc ttggtgttca ccaattgatt cttaaaatgc taacagttca taatgccagt
     1321 gtaaacttgt cagtgattgg actgaagacc ttagatctcc tcctaacttc aggtaaaatc
     1381 accttgctga tattggatga agaaagtgat attttcatgt taatttttga tgccatgcac
     1441 tcatttccag ccaatgatga agtccagaaa cttggatgca aagctttaca tgtgctgttt
     1501 gagagagtct cagaggagca actgactgaa tttgttgaga acaaagatta tatgatattg
     1561 ttaagtgcgt taacaaattt taaagatgaa gaggaaattg tgcttcatgt gctgcattgt
     1621 ttacattccc tagcgattcc ttgcaataat gtggaagtcc tcatgagtgg caatgtcagg
     1681 tgttataata ttgtggtgga agctatgaaa gcattcccta tgagtgaaag aattcaagaa
     1741 gtgagttgct gtttgctcca taggcttaca ttaggtaatt ttttcaatat cctggtatta
     1801 aacgaagtcc atgagtttgt ggtgaaagct gtgcagcagt acccagagaa tgcagcattg
     1861 cagatctcag cgctcagctg tttggccctc ctcactgaga ctattttctt aaatcaagat
     1921 ttagaggaaa agaatgagaa tcaagagaat gatgatgagg gggaagaaga taaattgttt
     1981 tggctggaag cctgttacaa agcattaacg tggcatagaa agaacaagca cgtgcaggag
     2041 gccgcatgct gggcactaaa taatctcctt atgtaccaaa acagtttaca tgagaagatt
     2101 ggagatgaag atggccattt cccagctcat agggaagtga tgctctccat gctgatgcat
     2161 tcttcatcaa aggaagtttt ccaggcatct gcgaatgcat tgtcaactct cttagaacaa
     2221 aatgttaatt tcagaaaaat actgttatca aaaggaatac acctgaatgt tttggagtta
     2281 atgcagaagc atatacattc tcctgaagtg gctgaaagtg gctgtaaaat gctaaatcat
     2341 ctttttgaag gaagcaacac ttccctggat ataatggcag cagtggtccc caaaatacta
     2401 acagttatga aacgtcatga gacatcatta ccagtgcagc tggaggcgct tcgagctatt
     2461 ttacatttta tagtgcctgg catgccagaa gaatccaggg aggatacaga atttcatcat
     2521 aagctaaata tggttaaaaa acagtgtttc aagaatgata ttcacaaact ggtcctagca
     2581 gctttgaaca ggttcattgg aaatcctggg attcagaaat gtggattaaa agtaatttct
     2641 tctattgtac attttcctga tgcattagag atgttatccc tggaaggtgc tatggattca
     2701 gtgcttcaca cactgcagat gtatccagat gaccaagaaa ttcagtgtct gggtttaagt
     2761 cttataggat acttgattac aaagaagaat gtgttcatag gaactggaca tctgctggca
     2821 aaaattctgg tttccagctt ataccgattt aaggatgttg ctgaaataca gactaaagga
     2881 tttcagacaa tcttagcaat cctcaaattg tcagcatctt tttctaagct gctggtgcat
     2941 cattcatttg acttagtaat attccatcaa atgtcttcca atatcatgga acaaaaggat
     3001 caacagtttc taaacctctg ttgcaagtgt tttgcaaaag tagctatgga tgattactta
     3061 aaaaatgtga tgctagagag agcgtgtgat cagaataaca gcatcatggt tgaatgcttg
     3121 cttctattgg gagcagatgc caatcaagca aaggagggat cttctttaat ttgtcaggta
     3181 tgtgagaaag agagcagtcc caaattggtg gaactcttac tgaatagtgg atctcgtgaa
     3241 caagatgtac gaaaagcgtt gacgataagc attgggaaag gtgacagcca gatcatcagc
     3301 ttgctcttaa ggaggctggc cctggatgtg gccaacaata gcatttgcct tggaggattt
     3361 tgtataggaa aagttgaacc ttcttggctt ggtcctttat ttccagataa gacttctaat
     3421 ttaaggaaac aaacaaatat agcatctaca ctagcaagaa tggtgatcag atatcagatg
     3481 aaaagtgctg tggaagaagg aacagcctca ggcagcgatg gaaatttttc tgaagatgtg
     3541 ctgtctaaat ttgatgaatg gacctttatt cctgactctt ctatggacag tgtgtttgct
     3601 caaagtgatg acctggatag tgaaggaagt gaaggctcat ttcttgtgaa aaagaaatct
     3661 aattcaatta gtgtaggaga attttaccga gatgccgtat tacagcgttg ctcaccaaat
     3721 ttgcaaagac attccaattc cttggggccc atttttgatc atgaagattt actgaagcga
     3781 aaaagaaaaa tattatcttc agatgattca ctcaggtcat caaaacttca atcccatatg
     3841 aggcattcag acagcatttc ttctctggct tctgagagag aatatattac atcactagac
     3901 ctttcagcaa atgaactaag agatattgat gccctaagcc agaaatgctg tataagtgtt
     3961 catttggagc atcttgaaaa gctggagctt caccagaatg cactcacgag ctttccacaa
     4021 cagctatgtg aaactctgaa gagtttgaca catttggact tgcacagtaa taaatttaca
     4081 tcatttcctt cttatttgtt gaaaatgagt tgtattgcta atcttgatgt ctctcgaaat
     4141 gacattggac cctcagtggt tttagatcct acagtgaaat gtccaactct gaaacagttt
     4201 aacctgtcat ataaccagct gtcttttgta cctgagaacc tcactgatgt ggtagagaaa
     4261 ctggagcagc tcattttaga aggaaataaa atatcaggga tatgctcccc cttgagactg
     4321 aaggaactga agattttaaa ccttagtaag aaccacattt catccctatc agagaacttt
     4381 cttgaggctt gtcctaaagt ggagagtttc agtgccagaa tgaattttct tgctgctatg
     4441 cctttcttgc ctccttctat gacaatccta aaattatctc agaacaaatt ttcctgtatt
     4501 ccagaagcaa ttttaaatct tccacacttg cggtctttag atatgagcag caatgatatt
     4561 cagtacctac caggtcccgc acactggaaa tctttgaact taagggaact cttatttagc
     4621 cataatcaga tcagcatctt ggacttgagt gaaaaagcat atttatggtc tagagtagag
     4681 aaactgcatc tttctcacaa taaactgaaa gagattcctc ctgagattgg ctgtcttgaa
     4741 aatctgacat ctctggatgt cagttacaac ttggaactaa gatcctttcc caatgaaatg
     4801 gggaaattaa gcaaaatatg ggatcttcct ttggatgaac tgcatcttaa ctttgatttt
     4861 aaacatatag gatgtaaagc caaagacatc ataaggtttc ttcaacagcg attaaaaaag
     4921 gctgtgcctt ataaccgaat gaaacttatg attgtgggaa atactgggag tggtaaaacc
     4981 accttattgc agcaattaat gaaaaccaag aaatcagatc ttggaatgca aagtgccaca
     5041 gttggcatag atgtgaaaga ctggcctatc caaataagag acaaaagaaa gagagatctc
     5101 gtcctaaatg tgtgggattt tgcaggtcgt gaggaattct atagtactca tccccatttt
     5161 atgacgcagc gagcattgta ccttgctgtc tatgacctca gcaagggaca ggctgaagtt
     5221 gatgccatga agccttggct cttcaatata aaggctcgcg cttcttcttc ccctgtgatt
     5281 ctcgttggca cacatttgga tgtttctgat gagaagcaac gcaaagcctg catgagtaaa
     5341 atcaccaagg aactcctgaa taagcgaggg ttccctgcca tacgagatta ccactttgtg
     5401 aatgccaccg aggaatctga tgctttggca aaacttcgga aaaccatcat aaacgagagc
     5461 cttaatttca agatccgaga tcagcttgtt gttggacagc tgattccaga ctgctatgta
     5521 gaacttgaaa aaatcatttt atcggagcgt aaaaatgtgc caattgaatt tcccgtaatt
     5581 gaccggaaac gattattaca actagtgaga gaaaatcagc tgcagttaga tgaaaatgag
     5641 cttcctcacg cagttcactt tctaaatgaa tcaggagtcc ttcttcattt tcaagaccca
     5701 gcactgcagt taagtgactt gtactttgtg gaacccaagt ggctttgtaa aatcatggca
     5761 cagattttga cagtgaaagt ggaaggttgt ccaaaacacc ctaagggcat tatttcgcgt
     5821 agagatgtgg aaaaatttct ttcaaaaaaa aggaaatttc caaagaacta catgtcacag
     5881 tattttaagc tcctagaaaa attccagatt gctttgccaa taggagaaga atatttgctg
     5941 gttccaagca gtttgtctga ccacaggcct gtgatagagc ttccccattg tgagaactct
     6001 gaaattatca tccgactata tgaaatgcct tattttccaa tgggattttg gtcaagatta
     6061 atcaatcgat tacttgagat ttcaccttac atgctttcag ggagagaacg agcacttcgc
     6121 ccaaacagaa tgtattggcg acaaggcatt tacttaaatt ggtctcctga agcttattgt
     6181 ctggtaggat ctgaagtctt agacaatcat ccagagagtt tcttaaaaat tacagttcct
     6241 tcttgtagaa aaggctgtat tcttttgggc caagttgtgg accacattga ttctctcatg
     6301 gaagaatggt ttcctgggtt gctggagatt gatatttgtg gtgaaggaga aactctgttg
     6361 aagaaatggg cattatatag ttttaatgat ggtgaagaac atcaaaaaat cttacttgat
     6421 gacttgatga agaaagcaga ggaaggagat ctcttagtaa atccagatca accaaggctc
     6481 accattccaa tatctcagat tgcccctgac ttgattttgg ctgacctgcc tagaaatatt
     6541 atgttgaata atgatgagtt ggaatttgaa caagctccag agtttctcct aggtgatggc
     6601 agttttggat cagtttaccg agcagcctat gaaggagaag aagtggctgt gaagattttt
     6661 aataaacata catcactcag gctgttaaga caagagcttg tggtgctttg ccacctccac
     6721 caccccagtt tgatatcttt gctggcagct gggattcgtc cccggatgtt ggtgatggag
     6781 ttagcctcca agggttcctt ggatcgcctg cttcagcagg acaaagccag cctcactaga
     6841 accctacagc acaggattgc actccacgta gctgatggtt tgagatacct ccactcagcc
     6901 atgattatat accgagacct gaaaccccac aatgtgctgc ttttcacact gtatcccaat
     6961 gctgccatca ttgcaaagat tgctgactac ggcattgctc agtactgctg tagaatgggg
     7021 ataaaaacat cagagggcac accagggttt cgtgcacctg aagttgccag aggaaatgtc
     7081 atttataacc aacaggctga tgtttattca tttggtttac tactctatga cattttgaca
     7141 actggaggta gaatagtaga gggtttgaag tttccaaatg agtttgatga attagaaata
     7201 caaggaaaat tacctgatcc agttaaagaa tatggttgtg ccccatggcc tatggttgag
     7261 aaattaatta aacagtgttt gaaagaaaat cctcaagaaa ggcctacttc tgcccaggtc
     7321 tttgacattt tgaattcagc tgaattagtc tgtctgacga gacgcatttt attacctaaa
     7381 aacgtaattg ttgaatgcat ggttgctaca catcacaaca gcaggaatgc aagcatttgg
     7441 ctgggctgtg ggcacaccga cagaggacag ctctcatttc ttgacttaaa tactgaagga
     7501 tacacttctg aggaagttgc tgatagtaga atattgtgct tagccttggt gcatcttcct
     7561 gttgaaaagg aaagctggat tgtgtctggg acacagtctg gtactctcct ggtcatcaat
     7621 accgaagatg ggaaaaagag acatacccta gaaaagatga ctgattctgt cacttgtttg
     7681 tattgcaatt ccttttccaa gcaaagcaaa caaaaaaatt ttcttttggt tggaaccgct
     7741 gatggcaagt tagcaatttt tgaagataag actgttaagc ttaaaggagc tgctcctttg
     7801 aagatactaa atataggaaa tgtcagtact ccattgatgt gtttgagtga atccacaaat
     7861 tcaacggaaa gaaatgtaat gtggggagga tgtggcacaa agattttctc cttttctaat
     7921 gatttcacca ttcagaaact cattgagaca agaacaagcc aactgttttc ttatgcagct
     7981 ttcagtgatt ccaacatcat aacagtggtg gtagacactg ctctctatat tgctaagcaa
     8041 aatagccctg ttgtggaagt gtgggataag aaaactgaaa aactctgtgg actaatagac
     8101 tgcgtgcact ttttaaggga ggtaatggta aaagaaaaca aggaatcaaa acacaaaatg
     8161 tcttattctg ggagagtgaa aaccctctgc cttcagaaga acactgctct ttggatagga
     8221 actggaggag gccatatttt actcctggat ctttcaactc gtcgacttat acgtgtaatt
     8281 tacaactttt gtaattcggt cagagtcatg atgacagcac agctaggaag ccttaaaaat
     8341 gtcatgctgg tattgggcta caaccggaaa aatactgaag gtacacaaaa gcagaaagag
     8401 atacaatctt gcttgaccgt ttgggacatc aatcttccac atgaagtgca aaatttagaa
     8461 aaacacattg aagtgagaaa agaattagct gaaaaaatga gacgaacatc tgttgagctc
     8521 gagaccggtg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
     8581 gatgacgatg acaagtgacc ggtaagttta aaccgctgat cagcctcgac tgtgccttct
     8641 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8701 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8761 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8821 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8881 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8941 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9001 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9061 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9121 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9181 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9241 gtctattctt ttgatttata agggattttg gccatttcgg cctattggtt aaaaaatgag
     9301 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     9361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9421 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9481 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9601 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9661 gcttttgcaa aaagctcccg ggatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9721 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9781 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9841 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     9901 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9961 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    10021 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    10081 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    10141 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    10201 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    10261 gacccgcaag cccggtgcct gattcgaaat gaccgaccaa gcgacgccca acctgccatc
    10321 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    10381 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    10441 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    10501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10561 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    10621 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10681 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10801 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10861 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    11041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    11101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    11161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    11221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    11281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    11341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    11461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11521 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
    11581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11641 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11701 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11761 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11821 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11881 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11941 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    12001 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    12061 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    12121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
