LOCUS       V047337                 8065 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047337
VERSION     V047337
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             913..3771
                     /label="Hdac7(NM_001204275)"
                     /note="Hdac7(NM_001204275)"
                     /gene="Hdac7"
     CDS             3790..3816
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3820..3846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3850..3876
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3929..4153
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4199..4627
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4641..4970
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4982..5581
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    5741..5862
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5911..5927)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5935..5951
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5959..5989)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6004..6025
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6313..6898)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7069..7929)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7930..8034)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagcttgcca ccatggacct gcgggtgggc cagcggccca cggtggagcc cccaccagag
      961 cctgcgctgc tgaccctgca acacccccaa cgcctgcacc gccatctctt cctggcaggc
     1021 ttacaccagc aacagcgctc agccgagccc atgaggctct ccatggaccc accaatgccg
     1081 gagctgcagg ggggacagca ggagcaagaa cttcggcaac ttctcaataa agacaagagc
     1141 aagcgaagtg ccgtagccag cagtgtggtc aagcagaagc tggctgaagt gatcctgaag
     1201 aaacagcagg cagcccttga gagaacagtc catcccagca gccccagtat tccctacaga
     1261 actcttgagc ccttggacac agagggtgct gcccgctccg tgcttagcag cttcctgcct
     1321 cctgttccca gcctgcccac tgaacccccg gaacactttc ccttgcgtaa aacagtgtct
     1381 gaacccaacc tgaagttgcg ctacaaaccc aagaaatccc tggagagacg caagaatccc
     1441 ctgctcagga aggagagtgc cccgcccagc cttcggagga ggcctgccga gacccttgga
     1501 gattcctccc ccagtagtag cagcacaccc gcgtcagggt gcagctcccc taatgacagc
     1561 gagcatggcc ctaaccctgc cctaggctca gaggcgctct tgggccagcg gctgcggctg
     1621 caggagactt ctctggcccc gttcgctttg ccgacagtgt ccttgctgcc cgcaatcaca
     1681 ctggggctgc ctgcccctgc cagggctgat ggtgaccgca ggacccattc aactttaggc
     1741 cctcggggtc ctgtactggg gaacccccat gctcccctct tcctgcacca cggtctggag
     1801 ccagaggctg ggggcacctt accctctcgc ctgcaaccca ttctcctgct ggacccctca
     1861 gtctctcatg ccccactgtg gactgtgcct ggccttgggc ccttgccctt ccactttgcc
     1921 cagcccttac tgaccaccga gcggctctct gggtcaggcc tccatcgacc acttaaccgg
     1981 acccgctcag agcccctgcc ccccagcgcc acagcctccc ctctgctggc ccccctgcag
     2041 ccccgccagg atcggctcaa acctcacgtc cagctgatca agccagccat ctcccctccc
     2101 cagaggcctg ccaagcccag tgagaagccc cgactgcgac agataccctc ggctgaggac
     2161 ctagagacag atggtggggg agtgggacct atggcgaatg atggcctgga acatagggag
     2221 tcaggccgtg ggcctcctga gggcagaggc tccatttctc tgcagcagca tcaacaggtg
     2281 ccaccctggg agcagcagca tctagccggg cggctctctc agggaagccc cggggactcc
     2341 gtgctgatac ctctggccca ggttggacac cggcccctgt ccagaaccca gtcttcccca
     2401 gcagcacctg tctccctgct gagcccagag cccacctgtc agacccaagt cctcaacagc
     2461 tcagagacac ctgctacagg gctggtctat gactcggtga tgctgaaaca ccaatgttcc
     2521 tgtggagaca acagcaagca tcccgagcat gcaggccgca tccagagcat ctggtcccgg
     2581 ctgcaggaac ggggtctccg cagccagtgt gagtgtctcc gaggccgaaa ggcttcccta
     2641 gaggagctgc agtcagtcca ctctgaacgg cacgtgctcc tctacggcac gaacccactc
     2701 agccgcctca aactggataa cgggaagctt acaggactcc tggcacagcg gacgtttgtg
     2761 atgctaccct gtggcggggt tggggtcgat actgacacca tctggaacga gctgcattcc
     2821 tccaatgcag cccgctgggc tgcgggcagt gtcaccgacc ttgccttcaa agtagcttcc
     2881 cgagagctga agaatggctt tgctgtggtg cgacccccgg gacaccatgc agatcattct
     2941 acagccatgg gcttctgctt cttcaactcc gtggccatcg cctgccgaca gctacagcaa
     3001 cacggcaaag ccagcaagat cctcattgtt gactgggatg ttcaccatgg caacggcaca
     3061 cagcagactt tctaccagga ccccagtgtg ctctacattt cccttcatcg ccatgacgac
     3121 ggcaacttct tcccaggcag tggggccgtg gatgaggtgg gaactggcag tggcgagggc
     3181 ttcaatgtca acgtggcttg ggctgggggc ttggatccac ccatggggga tcctgagtac
     3241 ctggctgctt tcaggatagt ggtgatgccc attgcccgag agtttgctcc agacctggtc
     3301 ctggtgtctg ctgggtttga tgctgcggag ggtcacccag ccccgctggg tggctaccat
     3361 gtttctgcca aatgttttgg gtacatgacg cagcagttga tgaacttggc aggaggcgcc
     3421 gtggtgttgg ccttagaggg tggacatgac ctcacggcca tctgtgatgc ctcggaggcc
     3481 tgtgtagctg ctcttctggg caacaaggtg gacccccttt cagaagaaag ctggaaacag
     3541 aaacccaacc tcagtgccat ccgctcgctg gaagctgtgg tcagggtgca caggaaatac
     3601 tggggctgca tgcagcgctt ggcctcctgt ccagactcct ggctacccag agtgccggga
     3661 gctgatgcag aagtggaagc cgtgaccgcg ctggcatccc tttctgtggg catcctggct
     3721 gaagacaggc cctcggagcg gctggtggaa gaggaagaac ccatgaacct ctaggaattc
     3781 gccaccatgt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     3841 tatgctagtt atccttatga tgtcccagac tacgcaggat ccggtaccct cgagtgaccg
     3901 gtaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4561 gggattttgg ccatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4981 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     5041 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     5101 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     5161 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     5221 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     5281 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5341 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5401 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5461 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5521 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5581 attcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5641 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5701 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5881 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6061 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6121 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6841 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8041 tccccgaaaa gtgccacctg acgtc
//
