LOCUS V047337 8065 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V047337 VERSION V047337 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 913..3771 /label="Hdac7(NM_001204275)" /note="Hdac7(NM_001204275)" /gene="Hdac7" CDS 3790..3816 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3820..3846 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3850..3876 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 3929..4153 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4199..4627 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4641..4970 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4982..5581 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 5741..5862 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5911..5927) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5935..5951 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5959..5989) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6004..6025 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6313..6898) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7069..7929) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7930..8034) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagcttgcca ccatggacct gcgggtgggc cagcggccca cggtggagcc cccaccagag 961 cctgcgctgc tgaccctgca acacccccaa cgcctgcacc gccatctctt cctggcaggc 1021 ttacaccagc aacagcgctc agccgagccc atgaggctct ccatggaccc accaatgccg 1081 gagctgcagg ggggacagca ggagcaagaa cttcggcaac ttctcaataa agacaagagc 1141 aagcgaagtg ccgtagccag cagtgtggtc aagcagaagc tggctgaagt gatcctgaag 1201 aaacagcagg cagcccttga gagaacagtc catcccagca gccccagtat tccctacaga 1261 actcttgagc ccttggacac agagggtgct gcccgctccg tgcttagcag cttcctgcct 1321 cctgttccca gcctgcccac tgaacccccg gaacactttc ccttgcgtaa aacagtgtct 1381 gaacccaacc tgaagttgcg ctacaaaccc aagaaatccc tggagagacg caagaatccc 1441 ctgctcagga aggagagtgc cccgcccagc cttcggagga ggcctgccga gacccttgga 1501 gattcctccc ccagtagtag cagcacaccc gcgtcagggt gcagctcccc taatgacagc 1561 gagcatggcc ctaaccctgc cctaggctca gaggcgctct tgggccagcg gctgcggctg 1621 caggagactt ctctggcccc gttcgctttg ccgacagtgt ccttgctgcc cgcaatcaca 1681 ctggggctgc ctgcccctgc cagggctgat ggtgaccgca ggacccattc aactttaggc 1741 cctcggggtc ctgtactggg gaacccccat gctcccctct tcctgcacca cggtctggag 1801 ccagaggctg ggggcacctt accctctcgc ctgcaaccca ttctcctgct ggacccctca 1861 gtctctcatg ccccactgtg gactgtgcct ggccttgggc ccttgccctt ccactttgcc 1921 cagcccttac tgaccaccga gcggctctct gggtcaggcc tccatcgacc acttaaccgg 1981 acccgctcag agcccctgcc ccccagcgcc acagcctccc ctctgctggc ccccctgcag 2041 ccccgccagg atcggctcaa acctcacgtc cagctgatca agccagccat ctcccctccc 2101 cagaggcctg ccaagcccag tgagaagccc cgactgcgac agataccctc ggctgaggac 2161 ctagagacag atggtggggg agtgggacct atggcgaatg atggcctgga acatagggag 2221 tcaggccgtg ggcctcctga gggcagaggc tccatttctc tgcagcagca tcaacaggtg 2281 ccaccctggg agcagcagca tctagccggg cggctctctc agggaagccc cggggactcc 2341 gtgctgatac ctctggccca ggttggacac cggcccctgt ccagaaccca gtcttcccca 2401 gcagcacctg tctccctgct gagcccagag cccacctgtc agacccaagt cctcaacagc 2461 tcagagacac ctgctacagg gctggtctat gactcggtga tgctgaaaca ccaatgttcc 2521 tgtggagaca acagcaagca tcccgagcat gcaggccgca tccagagcat ctggtcccgg 2581 ctgcaggaac ggggtctccg cagccagtgt gagtgtctcc gaggccgaaa ggcttcccta 2641 gaggagctgc agtcagtcca ctctgaacgg cacgtgctcc tctacggcac gaacccactc 2701 agccgcctca aactggataa cgggaagctt acaggactcc tggcacagcg gacgtttgtg 2761 atgctaccct gtggcggggt tggggtcgat actgacacca tctggaacga gctgcattcc 2821 tccaatgcag cccgctgggc tgcgggcagt gtcaccgacc ttgccttcaa agtagcttcc 2881 cgagagctga agaatggctt tgctgtggtg cgacccccgg gacaccatgc agatcattct 2941 acagccatgg gcttctgctt cttcaactcc gtggccatcg cctgccgaca gctacagcaa 3001 cacggcaaag ccagcaagat cctcattgtt gactgggatg ttcaccatgg caacggcaca 3061 cagcagactt tctaccagga ccccagtgtg ctctacattt cccttcatcg ccatgacgac 3121 ggcaacttct tcccaggcag tggggccgtg gatgaggtgg gaactggcag tggcgagggc 3181 ttcaatgtca acgtggcttg ggctgggggc ttggatccac ccatggggga tcctgagtac 3241 ctggctgctt tcaggatagt ggtgatgccc attgcccgag agtttgctcc agacctggtc 3301 ctggtgtctg ctgggtttga tgctgcggag ggtcacccag ccccgctggg tggctaccat 3361 gtttctgcca aatgttttgg gtacatgacg cagcagttga tgaacttggc aggaggcgcc 3421 gtggtgttgg ccttagaggg tggacatgac ctcacggcca tctgtgatgc ctcggaggcc 3481 tgtgtagctg ctcttctggg caacaaggtg gacccccttt cagaagaaag ctggaaacag 3541 aaacccaacc tcagtgccat ccgctcgctg gaagctgtgg tcagggtgca caggaaatac 3601 tggggctgca tgcagcgctt ggcctcctgt ccagactcct ggctacccag agtgccggga 3661 gctgatgcag aagtggaagc cgtgaccgcg ctggcatccc tttctgtggg catcctggct 3721 gaagacaggc cctcggagcg gctggtggaa gaggaagaac ccatgaacct ctaggaattc 3781 gccaccatgt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 3841 tatgctagtt atccttatga tgtcccagac tacgcaggat ccggtaccct cgagtgaccg 3901 gtaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 3961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 4021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 4081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 4141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 4201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 4261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 4321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 4381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 4441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 4501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 4561 gggattttgg ccatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 4621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 4681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 4741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 4801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 4861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 4921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 4981 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 5041 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 5101 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 5161 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 5221 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 5281 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 5341 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 5401 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 5461 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 5521 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 5581 attcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 5641 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 5701 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 5761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 5881 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 5941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6061 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6121 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 6481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 6661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 6721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 6781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 6841 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 7801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 7861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 7921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 7981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8041 tccccgaaaa gtgccacctg acgtc //