LOCUS       V046556                 7279 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046556
VERSION     V046556
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             928..954
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             958..984
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             988..1014
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1021..3099
                     /label="GMPS(NM_003875)"
                     /note="GMPS(NM_003875)"
                     /gene="GMPS"
     polyA_signal    3143..3367
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3413..3841
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3855..4184
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4196..4795
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    4955..5076
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5125..5141)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5149..5165
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5173..5203)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5218..5239
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5527..6112)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6283..7143)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7144..7248)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcgc caccatgtac ccatacgacg tacctgatta tgcaacttat
      961 ccttatgatg tacctgatta tgctagttat ccttatgatg tcccagacta cgcaggatcc
     1021 atggctctgt gcaacggaga ctccaagctg gagaatgctg gaggagacct taaggatggc
     1081 caccaccact atgaaggagc tgttgtcatt ctggatgctg gtgctcagta cgggaaagtc
     1141 atagaccgaa gagtgaggga actgttcgtg cagtctgaaa ttttcccctt ggaaacacca
     1201 gcatttgcta taaaggaaca aggattccgt gctattatca tctctggagg acctaattct
     1261 gtgtatgctg aagatgctcc ctggtttgat ccagcaatat tcactattgg caagcctgtt
     1321 cttggaattt gctatggtat gcagatgatg aataaggtat ttggaggtac tgtgcacaaa
     1381 aaaagtgtca gagaagatgg agttttcaac attagtgtgg ataatacatg ttcattattc
     1441 aggggccttc agaaggaaga agttgttttg cttacacatg gagatagtgt agacaaagta
     1501 gctgatggat tcaaggttgt ggcacgttct ggaaacatag tagcaggcat agcaaatgaa
     1561 tctaaaaagt tatatggagc acagttccac cctgaagttg gccttacaga aaatggaaaa
     1621 gtaatactga agaatttcct ttatgatata gctggatgca gtggaacctt caccgtgcag
     1681 aacagagaac ttgagtgtat tcgagagatc aaagagagag taggcacgtc aaaagttttg
     1741 gttttactca gtggtggagt agactcaaca gtttgtacag ctttgctaaa tcgtgctttg
     1801 aaccaagaac aagtcattgc tgtgcacatt gataatggct ttatgagaaa acgagaaagc
     1861 cagtctgttg aagaggccct caaaaagctt ggaattcagg tcaaagtgat aaatgctgct
     1921 cattctttct acaatggaac aacaacccta ccaatatcag atgaagatag aaccccacgg
     1981 aaaagaatta gcaaaacgtt aaatatgacc acaagtcctg aagagaaaag aaaaatcatt
     2041 ggggatactt ttgttaagat tgccaatgaa gtaattggag aaatgaactt gaaaccagag
     2101 gaggttttcc ttgcccaagg tactttacgg cctgatctaa ttgaaagtgc atcccttgtt
     2161 gcaagtggca aagctgaact catcaaaacc catcacaatg acacagagct catcagaaag
     2221 ttgagagagg agggaaaagt aatagaacct ctgaaagatt ttcataaaga tgaagtgaga
     2281 attttgggca gagaacttgg acttccagaa gagttagttt ccaggcatcc atttccaggt
     2341 cctggcctgg caatcagagt aatatgtgct gaagaacctt atatttgtaa ggactttcct
     2401 gaaaccaaca atattttgaa aatagtagct gatttttctg caagtgttaa aaagccacat
     2461 accctattac agagagtcaa agcctgcaca acagaagagg atcaggagaa gctgatgcaa
     2521 attaccagtc tgcattcact gaatgccttc ttgctgccaa ttaaaactgt aggtgtgcag
     2581 ggtgactgtc gttcctacag ttacgtgtgt ggaatctcca gtaaagatga acctgactgg
     2641 gaatcactta tttttctggc taggcttata cctcgcatgt gtcacaacgt taacagagtt
     2701 gtttatatat ttggcccacc agttaaagaa cctcctacag atgttactcc cactttcttg
     2761 acaacagggg tgctcagtac tttacgccaa gctgattttg aggcccataa cattctcagg
     2821 gagtctgggt atgctgggaa aatcagccag atgccggtga ttttgacacc attacatttt
     2881 gatcgggacc cacttcaaaa gcagccttca tgccagagat ctgtggttat tcgaaccttt
     2941 attactagtg acttcatgac tggtatacct gcaacacctg gcaatgagat ccctgtagag
     3001 gtggtattaa agatggtcac tgagattaag aagattcctg gtatttctcg aattatgtat
     3061 gacttaacat caaagccccc aggaactact gagtgggagt aactcgagtg accggtaagt
     3121 ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3181 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3241 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3301 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3361 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3421 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3481 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3541 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3601 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3661 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3721 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3781 ttggccattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3841 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3901 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     3961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4021 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4081 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4141 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggatgac
     4201 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4261 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4321 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4381 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4441 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4501 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4561 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4621 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4681 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4741 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgattcga
     4801 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     4861 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     4921 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     4981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5101 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5161 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5221 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5281 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5341 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5401 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5461 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5521 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5581 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5641 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5701 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5761 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5821 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5881 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5941 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6001 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6061 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     6361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     6421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     6601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     6721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     6781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     7021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     7261 aaaagtgcca cctgacgtc
//
