LOCUS       V019922                 7672 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019922
VERSION     V019922
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        67..370
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        371..574
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        620..638
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     CDS             679..705
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             721..4077
                     /label="WWC1(NM_001161661)"
                     /note="WWC1(NM_001161661)"
                     /gene="WWC1"
     promoter        complement(4174..4192)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     polyA_signal    4466..4587
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4594..5049)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5076..5180
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5182..5539
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5574..6368
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6600..6647
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6976..7564
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccatgta cccatacgat gttccagatt acgctagccc gggcggatcc
      721 atgccccggc cggagctgcc cctgccggag ggctgggagg aggcgcgcga cttcgacggc
      781 aaggtctact acatagacca cacgaaccgc accaccagct ggatcgaccc gcgggacagg
      841 tacaccaaac cgctcacctt tgctgactgc attagtgatg agttgccgct aggatgggaa
      901 gaggcatatg acccacaggt tggagattac ttcatagacc acaacaccaa aaccactcag
      961 attgaggatc ctcgagtaca atggcggcgg gagcaggaac atatgctgaa ggattacctg
     1021 gtggtggccc aggaggctct gagtgcacaa aaggagatct accaggtgaa gcagcagcgc
     1081 ctggagcttg cacagcagga gtaccagcaa ctgcatgccg tctgggagca taagctgggc
     1141 tcccaggtca gcttggtctc tggttcatca tccagctcca agtatgaccc tgagatcctg
     1201 aaagctgaaa ttgccactgc aaaatcccgg gtcaacaagc tgaagagaga gatggttcac
     1261 ctccagcacg agctgcagtt caaagagcgt ggctttcaga ccctgaagaa aatcgataag
     1321 aaaatgtctg atgctcaggg cagctacaaa ctggatgaag ctcaggctgt cttgagagaa
     1381 acaaaagcca tcaaaaaggc tattacctgt ggggaaaagg aaaagcaaga tctcattaag
     1441 agccttgcca tgttgaagga cggcttccgc actgacaggg ggtctcactc agacctgtgg
     1501 tccagcagca gctctctgga gagttcgagt ttcccgctac cgaaacagta cctggatgtg
     1561 agctcccaga cagacatctc gggaagcttc ggcatcaaca gcaacaatca gttggcagag
     1621 aaggtcagat tgcgccttcg atatgaagag gctaagagaa ggatcgccaa cctgaagatc
     1681 cagctggcca agcttgacag tgaggcctgg cctggggtgc tggactcaga gagggaccgg
     1741 ctgatcctta tcaacgagaa ggaggagctg ctgaaggaga tgcgcttcat cagcccccgc
     1801 aagtggaccc agggggaggt ggagcagctg gagatggccc ggaagcggct ggaaaaggac
     1861 ctgcaggcag cccgggacac ccagagcaag gcgctgacgg agaggttaaa gttaaacagt
     1921 aagaggaacc agcttgtgag agaactggag gaagccaccc ggcaggtggc aactctgcac
     1981 tcccagctga aaagtctctc aagcagcatg cagtccctgt cctcaggcag cagccccgga
     2041 tccctcacgt ccagccgggg ctccctggtt gcatccagcc tggactcctc cacttcagcc
     2101 agcttcactg acctctacta tgaccccttt gagcagctgg actcagagct gcagagcaag
     2161 gtggagttcc tgctcctgga gggggccacc ggcttccggc cctcaggctg catcaccacc
     2221 atccacgagg atgaggtggc caagacccag aaggcagagg gaggtggccg cctgcaggct
     2281 ctgcgttccc tgtctggcac cccaaagtcc atgacctccc tatccccacg ttcctctctc
     2341 tcctccccct ccccaccctg ttcccctctc atggctgacc ccctcctggc tggtgatgcc
     2401 ttcctcaact ccttggagtt tgaagacccg gagctgagtg ccactctttg tgaactgagc
     2461 cttggtaaca gcgcccagga aagataccgg ctggaggaac caggaacgga gggcaagcag
     2521 ctgggccaag ctgtgaatac ggcccagggg tgtggcctga aagtggcctg tgtctcagcc
     2581 gccgtatcgg acgagtcagt ggctggagac agtggtgtgt acgaggcttc cgtgcagaga
     2641 ctgggtgctt cagaagctgc tgcatttgac agtgacgaat cggaagcagt gggtgcgacc
     2701 cgaattcaga ttgccctgaa gtatgatgag aagaataagc aatttgcaat attaatcatc
     2761 cagctgagta acctttctgc tctgttgcag caacaagacc agaaagtgaa tatccgcgtg
     2821 gctgtccttc cttgctctga aagcacaacc tgcctgttcc ggacccggcc tctggacgcc
     2881 tcagacactc tagtgttcaa tgaggtgttc tgggtatcca tgtcctatcc agcccttcac
     2941 cagaagacct taagagtcga tgtctgtacc accgacagga gccatctgga agagtgcctg
     3001 ggaggcgccc agatcagcct ggcggaggtc tgccggtctg gggagaggtc gactcgctgg
     3061 tacaaccttc tcagctacaa atacttgaag aaacagagca gggagctcaa gccagtggga
     3121 gtcatggccc ctgcctcagg gcctgccagc acggacgctg tgtctgctct gttggaacag
     3181 acagcagtgg agctggagaa gaggcaggag ggcaggagca gcacacagac actggaagac
     3241 agctggaggt atgaggagac cagtgagaat gaggcagtag ccgaggaaga ggaggaggag
     3301 gtggaggagg aggagggaga agaggatgtt ttcaccgaga aagcctcacc tgatatggat
     3361 gggtacccag cattaaaggt ggacaaagag accaacacgg agaccccggc cccatccccc
     3421 acagtggtgc gacctaagga ccggagagtg ggcaccccgt cccaggggcc atttcttcga
     3481 gggagcacca tcatccgctc taagaccttc tccccaggac cccagagcca gtacgtgtgc
     3541 cggctgaatc ggagtgatag tgacagctcc actctgtcca aaaagccacc ttttgttcga
     3601 aactccctgg agcgacgcag cgtccggatg aagcggccgt ccccaccccc acagccttcc
     3661 tcggtcaagt cgctgcgctc cgagcgtctg atccgtacct cgctggacct ggagttagac
     3721 ctgcaggcga caagaacctg gcacagccaa ttgacccagg agatctcggt gctgaaggag
     3781 ctcaaggagc agctggaaca agccaagagc cacggggaga aggagctgcc acagtggttg
     3841 cgtgaggacg agcgtttccg cctgctgctg aggatgctgg agaagcggca gatggaccga
     3901 gcggagcaca agggtgagct tcagacagac aagatgatga gggcagctgc caaggatgtg
     3961 cacaggctcc gaggccagag ctgtaaggaa cccccagaag ttcagtcttt cagggagaag
     4021 atggcatttt tcacccggcc tcggatgaat atcccagctc tctctgcaga tgacgtctaa
     4081 gaattcgata tcgtcgacag atctctcgag tctagaacta gtgggcccgg taccttaatt
     4141 aattaaggta ccaggtaagt gtacccaatt cgccctatag tgagtcgtat tacaattcac
     4201 tcgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggagatcc aatttttaag
     4261 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc acagtcccaa
     4321 ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca gccataccac
     4381 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4441 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4501 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4561 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
     4621 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4681 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4741 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4801 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4861 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4921 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4981 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     5041 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5161 aatattgaaa aaggaagaat cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     5221 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5281 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5341 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5401 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5461 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5521 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     5581 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5641 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5701 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     5761 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5821 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5881 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5941 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6001 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagaacatc
     6061 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     6121 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6181 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6241 tggctacccg tgatattgct gaagaacttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6301 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6361 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     6421 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6481 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6541 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     6601 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     6661 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     6721 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     6781 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     6841 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6901 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6961 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7021 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7081 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7141 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7201 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7261 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7321 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7381 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7441 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7501 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7561 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7621 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cc
//
