LOCUS       V046600                 7422 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046600
VERSION     V046600
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..3310
                     /label="WFS1(NM_006005)"
                     /note="WFS1(NM_006005)"
                     /gene="WFS1"
     CDS             3368..4087
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    4210..4331
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4338..4793)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4820..4924
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4926..5283
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5318..6112
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6344..6391
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6720..7308
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggactcca acactgctcc
      661 gctgggcccc tcctgcccac agcccccgcc agcaccgcag ccccaggcgc gttcccgact
      721 caatgccaca gcctcgttgg agcaggagag gagcgaaagg ccccgagcac ccggacccca
      781 ggctggccct ggccctggtg ttagagacgc agcggccccc gctgaacccc aggcccagca
      841 taccaggagc cgggaaagag cagacggcac cgggcctaca aagggagaca tggaaatccc
      901 ctttgaagaa gtcctggaga gggccaaggc cggggacccc aaggcacaga ctgaggtggg
      961 gaagcactac ctgcagttgg ccggcgacac ggatgaagaa ctcaacagct gcaccgctgt
     1021 ggactggctg gtcctcgccg cgaagcaggg ccgtcgcgag gctgtgaagc tgcttcgccg
     1081 gtgcttggcg gacagaagag gcatcacgtc cgagaacgaa cgggaggtga ggcagctctc
     1141 ctccgagacc gacctggaga gggccgtgcg caaggcagcc ctggtcatgt actggaagct
     1201 caaccccaag aagaagaagc aggtggccgt ggcggagctg ctggagaatg tcggccaggt
     1261 caacgagcac gatggagggg cgcagccagg ccccgtgccc aagtccctgc agaagcagag
     1321 gcgcatgctg gagcgcctgg tcagcagcga gtccaagaac tacatcgcgc tggatgactt
     1381 tgtggagatc actaagaagt acgccaaggg cgtcatcccc agcagcctgt tcctgcagga
     1441 cgacgaagat gatgacgagc tggcggggaa gagccctgag gacctgccac tgcgtctgaa
     1501 ggtggtcaag taccccctgc acgccatcat ggagatcaag gagtacctga ttgacatggc
     1561 ctccagggca ggcatgcact ggctgtccac catcatcccc acgcaccaca tcaacgcgct
     1621 catcttcttc ttcatcgtca gcaacctcac catcgacttc ttcgccttct tcatcccgct
     1681 ggtcatcttc tacctgtcct tcatctccat ggtgatctgc accctcaagg tgttccagga
     1741 cagcaaggcc tgggagaact tccgcaccct caccgacctg ctgctgcgct tcgagcccaa
     1801 cctggatgtg gagcaggccg aggtcaactt cggctggaac cacctggagc cctatgccca
     1861 tttcctgctc tctgtcttct tcgtcatctt ctccttcccc atcgccagca aggactgcat
     1921 cccctgctcg gagctggctg tcatcaccgg cttctttacc gtgaccagct acctgagcct
     1981 gagcacccat gcagagccct acacgcgcag ggccctggcc accgaggtca ccgccggcct
     2041 gctatcgctg ctgccctcca tgcccttgaa ttggccctac ctgaaggtcc ttggccagac
     2101 cttcatcacc gtgcctgtcg gccacctggt cgtcctcaac gtcagcgtcc cgtgcctgct
     2161 ctatgtctac ctgctctatc tcttcttccg catggcacag ctgaggaatt tcaagggcac
     2221 ctactgctac cttgtgccct acctggtgtg cttcatgtgg tgtgagctct ccgtggtcat
     2281 cctgctggag tccaccggcc tggggctgct ccgcgcctcc atcggctact tcctcttcct
     2341 ctttgccctc cccatcctgg tggccggcct ggccctggtg ggcgtgctgc agttcgcccg
     2401 gtggttcacg tctctggagc tcaccaagat cgcagtcacc gtggcggtct gtagtgtgcc
     2461 cctgctgttg cgctggtgga ccaaggccag cttctctgtg gtggggatgg tgaagtccct
     2521 gacgcggagc tccatggtca agctcatcct ggtgtggctc acggccatcg tgctgttctg
     2581 ctggttctat gtgtaccgct cagagggcat gaaggtctac aactccacac tgacctggca
     2641 gcagtatggt gcgctgtgcg ggccacgcgc ctggaaggag accaacatgg cgcgcaccca
     2701 gatcctctgc agccacctgg agggccacag ggtcacgtgg accggccgct tcaagtacgt
     2761 ccgcgtgact gacatcgaca acagcgccga gtctgccatc aacatgctcc cgttcttcat
     2821 cggcgactgg atgcgctgcc tctacggcga ggcctaccct gcctgcagcc ctggcaacac
     2881 ctccacggcc gaggaggagc tctgtcgcct taagctgctg gccaagcacc cctgccacat
     2941 caagaagttc gaccgctaca agtttgagat taccgtgggc atgccattca gcagcggcgc
     3001 tgacggctcg cgcagccgcg aggaggacga cgtcaccaag gacatcgtgc tgcgggccag
     3061 cagcgagttc aagagcgtgc tgctcagcct gcgccagggc agcctcatcg agttcagcac
     3121 catcctggag ggccgcctgg gcagcaagtg gcctgtcttc gagctcaagg ccatcagctg
     3181 cctcaactgc atggcccagc tctcacccac caggcggcac gtgaagatcg agcacgactg
     3241 gcgcagcacc gtgcatggcg ccgtgaagtt cgccttcgac ttctttttct tcccattcct
     3301 gtcggcggcc gaattcggtg gaggcggatc aggaggcggt ggatctccgg atccaccggt
     3361 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3421 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3481 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3541 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
     3601 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3661 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3721 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3781 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3841 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3901 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3961 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4021 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4081 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     4141 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4201 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4261 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4321 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4381 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4441 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4501 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4561 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4621 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4681 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4741 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4801 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4921 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5041 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5101 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5161 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5221 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5281 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6121 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6181 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6241 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6301 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6361 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6421 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6481 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6541 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6601 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6661 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6721 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6781 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6841 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6901 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6961 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7021 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7081 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7141 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7201 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7321 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7381 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
