LOCUS V044446 6090 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044446 VERSION V044446 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1993 /label="TUBA1B(NM_006082)" /note="TUBA1B(NM_006082)" /gene="TUBA1B" CDS 2036..2755 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2878..2999 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3006..3461) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3488..3592 /gene="bla" /label="AmpR promoter" promoter 3594..3951 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3986..4780 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5012..5059 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5388..5976 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgcgtgagt gcatctccat 661 ccacgttggc caggctggtg tccagattgg caatgcctgc tgggagctct actgcctgga 721 acacggcatc cagcccgatg gccagatgcc aagtgacaag accattgggg gaggagatga 781 ctccttcaac accttcttca gtgagacggg cgctggcaag cacgtgcccc gggctgtgtt 841 tgtagacttg gaacccacag tcattgatga agttcgcact ggcacctacc gccagctctt 901 ccaccctgag cagctcatca caggcaagga agatgctgcc aataactatg cccgagggca 961 ctacaccatt ggcaaggaga tcattgacct tgtgttggac cgaattcgca agctggctga 1021 ccagtgcacc ggtcttcagg gcttcttggt tttccacagc tttggtgggg gaactggttc 1081 tgggttcacc tccctgctca tggaacgtct ctcagttgat tatggcaaga agtccaagct 1141 ggagttctcc atttacccag caccccaggt ttccacagct gtagttgagc cctacaactc 1201 catcctcacc acccacacca ccctggagca ctctgattgt gccttcatgg tagacaatga 1261 ggccatctat gacatctgtc gtagaaacct cgatatcgag cgcccaacct acactaacct 1321 taaccgcctt attagccaga ttgtgtcctc catcactgct tccctgagat ttgatggagc 1381 cctgaatgtt gacctgacag aattccagac caacctggtg ccctaccccc gcatccactt 1441 ccctctggcc acatatgccc ctgtcatctc tgctgagaaa gcctaccatg aacagctttc 1501 tgtagcagag atcaccaatg cttgctttga gccagccaac cagatggtga aatgtgaccc 1561 tcgccatggt aaatacatgg cttgctgcct gttgtaccgt ggtgacgtgg ttcccaaaga 1621 tgtcaatgct gccattgcca ccatcaaaac caagcgcagc atccagtttg tggattggtg 1681 ccccactggc ttcaaggttg gcatcaacta ccagcctccc actgtggtgc ctggtggaga 1741 cctggccaag gtacagagag ctgtgtgcat gctgagcaac accacagcca ttgctgaggc 1801 ctgggctcgc ctggaccaca agtttgacct gatgtatgcc aagcgtgcct ttgttcactg 1861 gtacgtgggt gaggggatgg aggaaggcga gttttcagag gcccgtgaag atatggctgc 1921 ccttgagaag gattatgagg aggttggtgt ggattctgtt gaaggagagg gtgaggaaga 1981 aggagaggaa tacggtggag gcggatcagg aggcggtgga tccccggtcg ccaccatggt 2041 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 2101 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 2161 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 2221 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 2281 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 2341 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 2401 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 2461 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 2521 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 2581 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 2641 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 2701 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg 2761 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 2821 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 2881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 2941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 3001 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 3061 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 3481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3541 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 3601 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3661 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3721 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3781 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3841 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3901 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 3961 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 4021 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 4081 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 4141 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 4201 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 4261 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 4321 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 4381 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 4441 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 4501 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 4561 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 4621 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 4681 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 4741 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 4801 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 4861 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 4921 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 4981 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 5041 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 5101 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 5161 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 5221 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 5281 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 5341 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5401 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5461 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 5521 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 5581 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 5641 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 5701 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 5761 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 5821 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 5881 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 5941 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 6001 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 6061 tctgtggata accgtattac cgccatgcat //