LOCUS       V019002                 6189 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019002
VERSION     V019002
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2068
                     /label="TFEB(NM_001271944)"
                     /note="TFEB(NM_001271944)"
                     /gene="TFEB"
     CDS             2135..2854
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2977..3098
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3105..3560)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3587..3691
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3693..4050
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4085..4879
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5111..5158
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5487..6075
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggcgtcac gcatagggtt
      661 gcgcatgcag ctcatgcggg agcaggcgca gcaggaggag cagcgggagc gcatgcagca
      721 acaggctgtc atgcattaca tgcagcagca gcagcagcag caacagcagc agctcggagg
      781 gccgcccacc ccggccatca atacccccgt ccacttccag tcgccaccac ctgtgcctgg
      841 ggaggtgttg aaggtgcagt cctacctgga gaatcccaca tcctaccatc tgcagcagtc
      901 gcagcatcag aaggtgcggg agtacctgtc cgagacctat gggaacaagt ttgctgccca
      961 catcagccca gcccagggct ctccgaaacc cccaccagcc gcctccccag gggtgcgagc
     1021 tggacacgtg ctgtcctcct ccgctggcaa cagtgctccc aatagcccca tggccatgct
     1081 gcacattggc tccaaccctg agagggagtt ggatgatgtc attgacaaca ttatgcgtct
     1141 ggacgatgtc cttggctaca tcaatcctga aatgcagatg cccaacacgc tacccctgtc
     1201 cagcagccac ctgaatgtgt acagcagcga cccccaggtc acagcctccc tggtgggcgt
     1261 caccagcagc tcctgccctg cggacctgac ccagaagcga gagctcacag atgctgagag
     1321 cagggccctg gccaaggagc ggcagaagaa agacaatcac aacttaattg aaaggagacg
     1381 aaggttcaac atcaatgacc gcatcaagga gttgggaatg ctgatcccca aggccaatga
     1441 cctggacgtg cgctggaaca agggcaccat cctcaaggcc tctgtggatt acatccggag
     1501 gatgcagaag gacctgcaaa agtccaggga gctggagaac cactctcgcc gcctggagat
     1561 gaccaacaag cagctctggc tccgtatcca ggagctggag atgcaggctc gagtgcacgg
     1621 cctccctacc acctccccgt ccggcatgaa catggctgag ctggcccagc aggtggtgaa
     1681 gcaggagctg cctagcgaag agggcccagg ggaggccctg atgctggggg ctgaggtccc
     1741 tgaccctgag ccactgccag ctctgccccc gcaagccccg ctgcccctgc ccacccagcc
     1801 accatcccca ttccatcacc tggacttcag ccacagcctg agctttgggg gcagggagga
     1861 cgagggtccc ccgggctacc ccgaacccct ggcgccgggg catggctccc cattccccag
     1921 cctgtccaag aaggatctgg acctcatgct cctggacgac tcactgctac cgctggcctc
     1981 tgatccactt ctgtccacca tgtcccccga ggcctccaag gccagcagcc gccggagcag
     2041 cttcagcatg gaggagggcg atgtgctggg cggcggaggg agtggaggag ggggaagcgg
     2101 aggcggaggg agtacggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     2161 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2221 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2281 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2341 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2401 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2461 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2521 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2581 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2641 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2701 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2761 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2821 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     2881 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2941 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3001 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3061 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     3121 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3181 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3241 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3301 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3361 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3421 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3481 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3541 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3661 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     3721 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3781 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3841 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3961 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4021 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4081 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4141 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4201 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4261 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4321 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4381 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4441 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4501 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4561 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4621 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4681 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4741 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4801 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4861 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     4921 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     4981 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5041 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     5101 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     5161 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5221 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5281 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     5341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6181 gccatgcat
//
