LOCUS       V018694                 8109 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018694
VERSION     V018694
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..4039
                     /label="RC3H1(NM_001300850)"
                     /note="RC3H1(NM_001300850)"
                     /gene="RC3H1"
     CDS             4055..4774
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4897..5018
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5025..5480)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5507..5611
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5613..5970
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6005..6799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7031..7078
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7407..7995
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgcctgtac aagctccaca
      661 atggacggat ttcctctcct gcccaatttg cactcagact ttcgacgaaa caattcgaaa
      721 gcccatcagt ttgggttgtg gccatactgt ctgcaagatg tgcctgaata aactccaccg
      781 caaggcttgc ccatttgacc agaccactat caatacagac attgagctcc tccctgtgaa
      841 ctcagcattg ctgcagctcg tgggtgctca ggtccctgag cagcagccta ttactttgtg
      901 tagtggggtt gaagacacaa agcattatga ggaagccaag aaatgtgtag aagaattagc
      961 attgtactta aaaccgctca gcagtgctag aggagtgggt ctgaacagca ctactcagag
     1021 tgttctgagt cgcccaatgc agaggaagct tgtgactcta gtccactgtc aactagtaga
     1081 agaagaaggc aggattcgtg ccatgagggc agctcgatct ttaggtgaac gaacagttac
     1141 agagctcatt ctccagcacc agaatcctca gcaactctct tccaatcttt gggcagcagt
     1201 aagggctagg ggatgccagt tccttggacc agcaatgcag gaggaagctt taaagttggt
     1261 tctgctggcc ttagaagatg gttctgcttt gtcaagaaaa gtattggttc tgtttgtggt
     1321 gcaaagattg gagccacggt ttcctcaagc ctctaaaact agcattgggc atgttgttca
     1381 gctcctttat agagcctcct gtttcaaggt caccaaacgt gatgaagact cttctttgat
     1441 gcagctgaaa gaagaattta gaacctatga agctctgcgg cgagaacatg actcccagat
     1501 agtgcagatt gctatggaag caggcttacg aattgcaccg gaccagtggt cctctttgct
     1561 ttatggagac cagtctcaca aatctcatat gcagtccatt attgacaagt tgcagactcc
     1621 agcctctttt gcacagagtg ttcaggaact aacaattgct ctccagcgaa ctggagaccc
     1681 agcaaacttg aaccgactaa gaccccattt ggagctgtta gcaaacattg accctagtcc
     1741 agatgctcct cctcctactt gggaacagct ggaaaatggg ctggttgctg tgcgtacagt
     1801 ggtacatggg ctggttgatt atatccagaa ccacagcaaa aaaggagcag atcagcagca
     1861 gcctccacag catagcaaat acaaaacata catgtgtcga gatatgaagc agagaggagg
     1921 atgccctcgt ggggccagct gtacatttgc acactcacag gaggaactgg aaaaatttcg
     1981 taaaatgaat aagcgcctgg ttccgagaag acccttgagt gcctctttgg gtcaacttaa
     2041 tgaggtgggc ctgccttcag cagctatcct tccagatgaa ggtgcagtgg atctccctag
     2101 cagaaaacct cctgctctgc caaatggaat tgtatcaaca gggaatacag taacacagct
     2161 tattccgcga gggacagacc ccagctatga ttctagtctg aaaccaggaa aaatagatca
     2221 tctgagcagt agtgctcctg gatcccctcc tgacctgcta gaatctgttc ctaagagtat
     2281 ttctgcctta cctgtgaatc cacattctat acctccaagg gggccagcag atctgcctcc
     2341 aatgcctgtt accaaaccac ttcagatggt acctcgaggt tctcagttat atccagcaca
     2401 acagacggat gtttattatc aggatcctcg aggagcagct ccgccatttg aaccagcacc
     2461 ttatcagcag ggtatgtatt atactccacc accacaatgt gtgtcccgct ttgtccgacc
     2521 tccaccatct gctcctgaac ctgctcctcc ctacttggat cattatccac cctacctcca
     2581 agaacgtgtt gtaaactctc agtatggcac acagccacag cagtacccac ctatataccc
     2641 atctcactat gatggccgtc gagtgtaccc tgctccgtct tacacaagag aagagatatt
     2701 ccgagaaagc cctataccca ttgagattcc acctgcagca gtaccatcgt atgtaccaga
     2761 atccagagaa agataccaac agatcgagag ttactatcca gtggctcctc atccaactca
     2821 gatcagacct tcgtacctca gagaacctcc ttatagccgg cttcctcctc ctccccagcc
     2881 tcatcctagt ctagatgaac tacatcgccg acgaaaggaa ataatggccc agctagagga
     2941 aagaaaggtt atctctccac ctccttttgc accttcacca accttgcctc ctacctttca
     3001 tccggaagaa tttttggatg aagacttgaa ggtagctggg aaatacaaag gaaatgatta
     3061 tagccaatac tctccctggt catgtgacac catcggctcc tacattggaa ccaaagatgc
     3121 aaaacccaaa gatgttgtgg cagcagggag tgtggaaatg atgaatgtgg agagtaaagg
     3181 aatgagggac cagcgattag atcttcagag aagagcagca gaaaccagtg atgatgacct
     3241 catcccattt ggagaccgac caacagtgtc tcggtttggt gccatctcac gaacttccaa
     3301 aactatatat cagggtgctg gtccaatgca ggctatggca cctcagggag ctcctacaaa
     3361 atctattaac atttcagatt atagtccata tggaacccac ggtggctggg gagcttctcc
     3421 atattcacct catcaaaaca taccttctca gggacacttc agtgagaggg agagaatatc
     3481 tatgtcagaa gtggccagtc atggaaaacc ccttccatct gctgagagag aacagctacg
     3541 actagaattg cagcaattga accatcagat tagccagcag acccagctac gtggactaga
     3601 ggctgttagt aacaggctgg tgttgcagag ggaggcaaac accctggcag gccagtcaca
     3661 gcccccacca ccgccacctc caaaatggcc tgggatgatc tcaagtgagc agttgagctt
     3721 ggaactgcac caggtggaaa gggaaatcgg gaagagaaca cgggaactga gtatggaaaa
     3781 ccagtgttct ctggacatga aaagcaaact gaatacaagt aaacaagcag aaaatggaca
     3841 accagaacca caaaacaagg ttccggctga ggaccttaca ttgacattca gtgatgtacc
     3901 aaatggatca gccttgacac aagagaatat cagcctccta tcaaacaaga ccagctctct
     3961 gaacctgtca gaggaccctg agggaggagg ggataataat gactcccaga gatcaggagt
     4021 tactcccagt tctgctcctt caccggtcgc caccatggtg agcaagggcg aggagctgtt
     4081 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4141 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4201 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4261 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4321 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4381 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4441 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4501 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4561 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4621 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4681 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4741 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     4801 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4861 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4981 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     5041 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     5101 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     5161 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     5221 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     5281 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     5341 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     5401 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     5461 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5581 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     5641 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5701 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5761 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5941 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     6001 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6061 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6121 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6181 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6241 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     6301 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     6361 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     6421 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     6481 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     6541 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     6601 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     6661 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6721 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6781 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6841 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6901 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6961 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     7021 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     7081 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     7141 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     7201 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     7261 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7321 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7381 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7441 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7501 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7561 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7621 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7681 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7741 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7801 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7861 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7921 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7981 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8041 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8101 gccatgcat
//
