LOCUS V046712 5496 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046712 VERSION V046712 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..1384 /label="PSMA7(NM_002792)" /note="PSMA7(NM_002792)" /gene="PSMA7" CDS 1442..2161 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced YFP" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EYFP" polyA_signal 2284..2405 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2412..2867) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2894..2998 /gene="bla" /label="AmpR promoter" promoter 3000..3357 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3392..4186 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4418..4465 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4794..5382 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgagctacg accgcgccat 661 caccgtcttc tcgcccgacg gccacctctt ccaagtggag tacgcgcagg aggccgtcaa 721 gaagggctcg accgcggttg gtgttcgagg aagagacatt gttgttcttg gtgtggagaa 781 gaagtcagtg gccaaactgc aggatgaaag aacagtgcgg aagatctgtg ctttggatga 841 caacgtctgc atggcctttg caggcctcac cgccgatgca aggatagtca tcaacagggc 901 ccgggtggag tgccagagcc accggctgac tgtggaggac ccggtcactg tggagtacat 961 cacccgctac atcgccagtc tgaagcagcg ttatacgcag agcaatgggc gcaggccgtt 1021 tggcatctct gccctcatcg tgggtttcga ctttgatggc actcctaggc tctatcagac 1081 tgacccctcg ggcacatacc atgcctggaa ggccaatgcc ataggtcggg gtgccaagtc 1141 agtgcgcgag ttcctggaga agaactatac tgacgaagcc attgaaacag atgatctgac 1201 cattaagctg gtgatcaagg cactcctgga agtggttcag tcaggtggca aaaacattga 1261 acttgctgtc atgaggcgag atcaatccct caagatttta aatcctgaag aaattgagaa 1321 gtatgttgct gaaattgaaa aagaaaaaga agaaaacgaa aagaagaaac aaaagaaagc 1381 atcagaattc ggtggaggcg gatcaggagg cggtggatct ccggatccac cggtcgccac 1441 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 1501 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 1561 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 1621 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa 1681 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 1741 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 1801 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 1861 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 1921 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 1981 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 2041 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 2101 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 2161 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 2221 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 2281 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 2341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 2401 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 2461 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 2521 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 2581 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 2641 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 2701 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 2761 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2821 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 2881 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2941 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 3001 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3181 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3241 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 3301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 3361 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 3421 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 3481 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 3541 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 3601 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 3661 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 3721 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3781 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3841 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 3901 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 3961 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4021 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4081 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 4141 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 4201 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 4261 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 4321 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 4381 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 4441 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 4501 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 4561 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 4621 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 4681 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4741 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4801 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4861 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4921 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 4981 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5041 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5101 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5161 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5221 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5281 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5341 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5401 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 5461 cctgattctg tggataaccg tattaccgcc atgcat //