LOCUS       V028151                 6296 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028151
VERSION     V028151
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        597..615
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     CDS             653..2122
                     /label="PLD3(NM_001031696)"
                     /note="PLD3(NM_001031696)"
                     /gene="PLD3"
     CDS             2153..2872
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        complement(2936..2954)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     polyA_signal    3084..3205
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3212..3667)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3694..3798
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3800..4157
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4192..4986
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5218..5265
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5594..6182
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaatt
      601 aaccctcact aaagggaaca aaagctggag ctctagcgct accggtgcca ccatgaagcc
      661 taaactgatg taccaggagc tgaaggtgcc tgcagaggag cccgccaatg agctgcccat
      721 gaatgagatt gaggcgtgga aggctgcgga aaagaaagcc cgctgggtcc tgctggtcct
      781 cattctggcg gttgtgggct tcggagccct gatgactcag ctgtttctat gggaatacgg
      841 cgacttgcat ctctttgggc ccaaccagcg cccagccccc tgctatgacc cttgcgaagc
      901 agtgctggtg gaaagcattc ctgagggcct ggacttcccc aatgcctcca cggggaaccc
      961 ttccaccagc caggcctggc tgggcctgct cgccggtgcg cacagcagcc tggacatcgc
     1021 ctccttctac tggaccctca ccaacaatga cacccacacg caggagccct ctgcccagca
     1081 gggtgaggag gtcctccggc agctgcagac cctggcacca aagggcgtga acgtccgcat
     1141 cgctgtgagc aagcccagcg ggccccagcc acaggcggac ctgcaggctc tgctgcagag
     1201 cggtgcccag gtccgcatgg tggacatgca gaagctgacc catggcgtcc tgcataccaa
     1261 gttctgggtg gtggaccaga cccacttcta cctgggcagt gccaacatgg actggcgttc
     1321 actgacccag gtcaaggagc tgggcgtggt catgtacaac tgcagctgcc tggctcgaga
     1381 cctgaccaag atctttgagg cctactggtt cctgggccag gcaggcagct ccatcccatc
     1441 aacttggccc cggttctatg acacccgcta caaccaagag acaccaatgg agatctgcct
     1501 caatggaacc cctgctctgg cctacctggc gagtgcgccc ccacccctgt gtccaagtgg
     1561 ccgcactcca gacctgaagg ctctactcaa cgtggtggac aatgcccgga gtttcatcta
     1621 cgtcgctgtc atgaactacc tgcccactct ggagttctcc caccctcaca ggttctggcc
     1681 tgccattgac gatgggctgc ggcgggccac ctacgagcgt ggcgtcaagg tgcgcctgct
     1741 catcagctgc tggggacact cggagccatc catgcgggcc ttcctgctct ctctggctgc
     1801 cctgcgtgac aaccataccc actctgacat ccaggtgaaa ctctttgtgg tccccgcgga
     1861 tgaggcccag gctcgaatcc catatgcccg tgtcaaccac aacaagtaca tggtgactga
     1921 acgcgccacc tacatcggaa cctccaactg gtctggcaac tacttcacgg agacggcggg
     1981 cacctcgctg ctggtgacgc agaatgggag gggcggcctg cggagccagc tggaggccat
     2041 tttcctgagg gactgggact ccccttacag ccatgacctt gacacctcag ctgacagcgt
     2101 gggcaacgcc tgccgcctgc tcggcggtgg aggaagcggc ggaggtggat ctatggtgag
     2161 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     2221 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     2281 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     2341 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     2401 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     2461 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     2521 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     2581 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     2641 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2701 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2761 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2821 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt agtctagaac
     2881 tagtgggccc ggtaccttaa ttaattaagg taccaggtaa gtgtacccaa ttcgccctat
     2941 agtgagtcgt attaggatcc accggatcta gataactgat cataatcagc cataccacat
     3001 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3061 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3121 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3181 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     3241 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3301 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3361 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3421 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3481 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3541 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3601 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3661 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3721 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3781 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     3841 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3901 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3961 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4021 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4081 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4141 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     4201 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4261 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4321 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     4381 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4441 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4501 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4561 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4621 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4681 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     4741 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4801 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4861 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4921 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4981 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5041 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5101 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     5161 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     5221 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     5281 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     5341 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     5401 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     5461 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5521 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5581 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5641 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5701 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5761 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5821 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5881 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5941 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6001 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6061 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6121 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6181 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6241 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
