LOCUS       V041465                 7782 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041465
VERSION     V041465
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..3676
                     /label="PHF20(NM_016436)"
                     /note="PHF20(NM_016436)"
                     /gene="PHF20"
     CDS             3728..4447
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4570..4691
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4698..5153)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5180..5284
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5286..5643
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5678..6472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6704..6751
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7080..7668
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgacaaagc atccacctaa
      661 cagacgagga atcagctttg aagtgggagc ccagttggaa gcccgggacc gtttaaaaaa
      721 ctggtatcca gctcacatag aagacattga ctacgaggaa ggaaaagtac tcatccattt
      781 caagcgttgg aaccatcgtt atgatgagtg gttctgctgg gacagtcctt atttacgccc
      841 tttagagaaa atacagctga ggaaagaggg cttgcatgaa gaggatggat cttctgaatt
      901 tcaaataaat gagcaggtcc ttgcttgctg gtctgattgt cgtttttacc cggccaaagt
      961 cactgctgtt aacaaggatg gtacttacac tgtgaaattt tatgatggag tagttcagac
     1021 tgtcaaacat attcatgtca aagctttttc caaagatcag aatattgtgg gtaatgctag
     1081 gcctaaagaa acagatcaca aaagtctttc atcatctcct gataaacgag agaagtttaa
     1141 agaacagaga aaagcaacag tgaatgtgaa gaaagacaaa gaagataaac ccttaaagac
     1201 agaaaagcga cccaagcagc ctgataaaga aggaaagtta atctgttctg aaaaggggaa
     1261 agtgtcagag aaaagtcttc ccaagaacga gaaggaagac aaggaaaaca tttccgaaaa
     1321 tgacagagag tattctggag atgcccaagt ggataagaaa cctgaaaatg acattgtgaa
     1381 gagtccacaa gaaaacttga gggaacccaa aagaaaacga ggcagacccc cttccatagc
     1441 tcctactgct gtggattcaa actctcaaac tttgcaacca ataacattgg aactgagaag
     1501 aaggaaaata tcaaaaggat gtgaagtccc attaaaacgt cctcggcttg acaaaaattc
     1561 atcccaggaa aagtcaaaaa actactcgga aaacactgac aaagacttat cgaggagacg
     1621 ttcctccagg ctgtccacta atgggaccca tgagatccta gatcctgact tggttgtatc
     1681 agatttggtt gatacggatc ctttgcaaga cacgttgtct agtaccaagg aatctgaaga
     1741 aggtcagttg aagtctgctt tggaagctgg ccaggtctca tctgcactga cttgccactc
     1801 ctttggggat ggatccgggg ctgcaggctt ggagttgaac tgcccatcaa tgggagaaaa
     1861 cacgatgaaa acagaaccga cttctcccct tgtggaatta caagagattt cgactgtgga
     1921 agtaacaaat acttttaaga aaacagatga ttttgggtca tctaatgcac cagctgtcga
     1981 cctagaccat aagtttagat gcaaagttgt ggactgttta aaatttttcc gcaaagccaa
     2041 actgttgcac tatcacatga agtatttcca tggaatggag aagtcactgg agccagaaga
     2101 gagcccggga aagaggcatg tccaaaccag gggcccttca gcttcagaca agcccagcca
     2161 ggagaccctg accaggaagc gggtctctgc cagttcccca actacaaaag acaaggaaaa
     2221 gaataaagag aagaaattca aggagtttgt gagagtgaag ccaaagaaga aaaagaaaaa
     2281 gaaaaagaaa accaaacctg aatgcccctg cagtgaggag atcagtgaca cctcccagga
     2341 accttctcca cccaaggcat ttgctgttac caggtgtggg tcctcacaca agccaggggt
     2401 ccatatgagc ccgcagcttc atggcccaga atctggacac cacaaaggga aagtgaaagc
     2461 attggaggag gataatttga gtgagtcctc ttctgagagc tttctctgga gtgatgatga
     2521 gtatggccaa gatgtggatg tgaccaccaa cccagatgag gaacttgatg gggatgaccg
     2581 ctatgacttc gaggtggtcc gctgcatctg tgaggtccag gaggaaaatg acttcatgat
     2641 tcagtgtgaa gagtgccagt gctggcagca tggggtctgc atgggattac tggaagaaaa
     2701 tgtgcccgag aaatacacct gttatgtttg ccaagaccct ccaggtcaga ggcctggctt
     2761 caagtactgg tatgacaagg agtggctgag caggggacat atgcatggcc tggcatttct
     2821 agaagagaac tactcccatc agaatgccaa gaagatcgtg gccacccacc agcttcttgg
     2881 tgatgtgcag agagtgattg aggttctgca tggcctgcag ctcaagatga gcatcttgca
     2941 aagccgggag catcctgatc tgccgctgtg gtgccagcct tggaaacagc actcagggga
     3001 ggggagatct catttcagaa acatccctgt cactgacacc aggagcaagg aggaagctcc
     3061 aagctataga actttgaacg gggcagtgga gaagcccagg cccctggccc tgcccctgcc
     3121 gcgttctgtg gaggaatcct atatcaccag tgagcattgc taccagaagc cccgcgccta
     3181 ttaccctgcc gtggagcaga agctggtggt ggagacgagg ggctctgccc tcgacgatgc
     3241 ggtcaacccc ctccatgaga acggcgatga ttccctttcc ccgcgcctgg gctggcctct
     3301 agaccaagac aggagcaagg gggacagtga ccccaaaccc ggctccccaa aggtgaagga
     3361 atatgtctcc aaaaaggccc taccagaaga agcccctgct cggaagctgc tggacagagg
     3421 tggagagggg ctgctgagct cccagcacca gtggcagttt aacctgctga cccatgtgga
     3481 atctcttcag gatgaagtta cgcacaggat ggactccatt gagaaggagt tggatgtgtt
     3541 ggagagctgg ctggactaca ctggggaact ggagccccct gagccgctgg ccaggcttcc
     3601 gcagctcaag cattgtatca agcagctgct gatggacctg ggcaaggtgc agcagatcgc
     3661 cctctgctgc tcaacaggtg gaggcggatc aggaggcggt ggatctgcgg atccaccggt
     3721 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3781 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3841 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3901 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3961 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4021 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4081 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     4141 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     4201 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     4261 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4321 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     4381 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     4441 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     4501 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4561 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4681 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4741 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4801 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4861 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4921 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4981 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5041 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5101 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5281 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5401 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5461 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5521 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5641 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5701 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5761 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5821 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     5881 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5941 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6001 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6061 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6121 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6181 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     6241 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6301 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6361 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6421 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6481 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6601 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6661 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6721 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6781 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6841 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6901 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7741 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
