LOCUS       V027998                 6235 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027998
VERSION     V027998
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        67..370
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        371..574
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        620..638
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     regulatory      673..682
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             682..705
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             721..2640
                     /label="PCK2(NM_004563)"
                     /note="PCK2(NM_004563)"
                     /gene="PCK2"
     primer_bind     complement(2664..2680)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="KS primer"
     promoter        complement(2737..2755)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     polyA_signal    3029..3150
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3157..3612)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3639..3743
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3745..4102
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4137..4931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5163..5210
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5539..6127
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagagccc gggcggatcc
      721 atggccgcat tgtaccgccc tggcctgcgg cttaactggc atgggctgag ccccttgggc
      781 tggccatcat gccgtagcat ccagaccctg cgagtgctta gtggagatct gggccagctt
      841 cccactggca ttcgagattt tgtagagcac agtgcccgcc tgtgccaacc agagggcatc
      901 cacatctgtg atggaactga ggctgagaat actgccacac tgaccctgct ggagcagcag
      961 ggcctcatcc gaaagctccc caagtacaat aactgctggc tggcccgcac agaccccaag
     1021 gatgtggcac gagtagagag caagacggtg attgtaactc cttctcagcg ggacacggta
     1081 ccactcccgc ctggtggggc ccgtgggcag ctgggcaact ggatgtcccc agctgatttc
     1141 cagcgagctg tggatgagag gtttccaggc tgcatgcagg gccgcaccat gtatgtgctt
     1201 ccattcagca tgggtcctgt gggctccccg ctgtcccgca tcggggtgca gctcactgac
     1261 tcagcctatg tggtggcaag catgcgtatt atgacccgac tggggacacc tgtgcttcag
     1321 gccctgggag atggtgactt tgtcaagtgt ctgcactccg tgggccagcc cctgacagga
     1381 caaggggagc cagtgagcca gtggccgtgc aacccagaga aaaccctgat tggccacgtg
     1441 cccgaccagc gggagatcat ctccttcggc agcggctatg gtggcaactc cctgctgggc
     1501 aagaagtgct ttgccctacg catcgcctct cggctggccc gggatgaggg ctggctggca
     1561 gagcacatgc tgatcctggg catcaccagc cctgcaggga agaagcgcta tgtggcagcc
     1621 gccttcccta gtgcctgtgg caagaccaac ctggctatga tgcggcctgc actgccaggc
     1681 tggaaagtgg agtgtgtggg ggatgatatt gcttggatga ggtttgacag tgaaggtcga
     1741 ctccgggcca tcaaccctga gaacggcttc tttggggttg cccctggtac ctctgccacc
     1801 accaatccca acgccatggc tacaatccag agtaacacta tttttaccaa tgtggctgag
     1861 accagtgatg gtggcgtgta ctgggagggc attgaccagc ctcttccacc tggtgttact
     1921 gtgacctcct ggctgggcaa accctggaaa cctggtgaca aggagccctg tgcacatccc
     1981 aactctcgat tttgtgcccc ggctcgccag tgccccatca tggacccagc ctgggaggcc
     2041 ccagagggtg tccccattga cgccatcatc tttggtggcc gcagacccaa aggggtaccc
     2101 ctggtatacg aggccttcaa ctggcgtcat ggggtgtttg tgggcagcgc catgcgctct
     2161 gagtccactg ctgcagcaga acacaaaggg aagatcatca tgcacgaccc atttgccatg
     2221 cggccctttt ttggctacaa cttcgggcac tacctggaac actggctgag catggaaggg
     2281 cgcaaggggg cccagctgcc ccgtatcttc catgtcaact ggttccggcg tgacgaggca
     2341 gggcacttcc tgtggccagg ctttggggag aatgctcggg tgctagactg gatctgccgg
     2401 cggttagagg gggaggacag tgcccgagag acacccattg ggctggtgcc aaaggaagga
     2461 gccttggatc tcagcggcct cagagctata gacaccactc agctgttctc cctccccaag
     2521 gacttctggg aacaggaggt tcgtgacatt cggagctacc tgacagagca ggtcaaccag
     2581 gatctgccca aagaggtgtt ggctgagctt gaggccctgg agagacgtgt gcacaaaatg
     2641 tgagaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacctta
     2701 attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg tattacaatt
     2761 cactcgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaga tccaattttt
     2821 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttcacagtcc
     2881 caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac
     2941 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3001 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3061 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3121 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt
     3181 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     3241 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     3301 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     3361 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     3421 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     3481 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     3541 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     3601 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3661 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3721 aataatattg aaaaaggaag aatcctgagg cggaaagaac cagctgtgga atgtgtgtca
     3781 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3841 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3901 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3961 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4021 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4081 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     4141 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4201 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4261 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     4321 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4381 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4441 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4501 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4561 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagaac
     4621 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     4681 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4741 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4801 cgttggctac ccgtgatatt gctgaagaac ttggcggcga atgggctgac cgcttcctcg
     4861 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4921 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4981 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     5041 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     5101 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5161 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     5221 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     5281 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     5341 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     5401 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5461 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5521 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5581 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5641 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     5701 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5761 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5821 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5881 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5941 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6001 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6061 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6121 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6181 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcc
//
