LOCUS       V026544                 6681 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026544
VERSION     V026544
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2578
                     /label="MCAM(NM_006500)"
                     /note="MCAM(NM_006500)"
                     /gene="MCAM"
     CDS             2627..3346
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced blue variant of GFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EBFP"
     polyA_signal    3469..3590
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3597..4052)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4079..4183
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4185..4542
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4577..5371
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5603..5650
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5979..6567
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggggcttc ccaggctggt
      661 ctgcgccttc ttgctcgccg cctgctgctg ctgtcctcgc gtcgcgggtg tgcccggaga
      721 ggctgagcag cctgcgcctg agctggtgga ggtggaagtg ggcagcacag cccttctgaa
      781 gtgcggcctc tcccagtccc aaggcaacct cagccatgtc gactggtttt ctgtccacaa
      841 ggagaagcgg acgctcatct tccgtgtgcg ccagggccag ggccagagcg aacctgggga
      901 gtacgagcag cggctcagcc tccaggacag aggggctact ctggccctga ctcaagtcac
      961 cccccaagac gagcgcatct tcttgtgcca gggcaagcgc cctcggtccc aggagtaccg
     1021 catccagctc cgcgtctaca aagctccgga ggagccaaac atccaggtca accccctggg
     1081 catccctgtg aacagtaagg agcctgagga ggtcgctacc tgtgtaggga ggaacgggta
     1141 ccccattcct caagtcatct ggtacaagaa tggccggcct ctgaaggagg agaagaaccg
     1201 ggtccacatt cagtcgtccc agactgtgga gtcgagtggt ttgtacacct tgcagagtat
     1261 tctgaaggca cagctggtta aagaagacaa agatgcccag ttttactgtg agctcaacta
     1321 ccggctgccc agtgggaacc acatgaagga gtccagggaa gtcaccgtcc ctgttttcta
     1381 cccgacagaa aaagtgtggc tggaagtgga gcccgtggga atgctgaagg aaggggaccg
     1441 cgtggaaatc aggtgtttgg ctgatggcaa ccctccacca cacttcagca tcagcaagca
     1501 gaaccccagc accagggagg cagaggaaga gacaaccaac gacaacgggg tcctggtgct
     1561 ggagcctgcc cggaaggaac acagtgggcg ctatgaatgt cagggcctgg acttggacac
     1621 catgatatcg ctgctgagtg aaccacagga actactggtg aactatgtgt ctgacgtccg
     1681 agtgagtccc gcagcccctg agagacagga aggcagcagc ctcaccctga cctgtgaggc
     1741 agagagtagc caggacctcg agttccagtg gctgagagaa gagacaggcc aggtgctgga
     1801 aagggggcct gtgcttcagt tgcatgacct gaaacgggag gcaggaggcg gctatcgctg
     1861 cgtggcgtct gtgcccagca tacccggcct gaaccgcaca cagctggtca acgtggccat
     1921 ttttggcccc ccttggatgg cattcaagga gaggaaggtg tgggtgaaag agaatatggt
     1981 gttgaatctg tcttgtgaag cgtcagggca cccccggccc accatctcct ggaacgtcaa
     2041 cggcacggca agtgaacaag accaagatcc acagcgagtc ctgagcaccc tgaatgtcct
     2101 cgtgaccccg gagctgttgg agacaggtgt tgaatgcacg gcctccaacg acctgggcaa
     2161 aaacaccagc atcctcttcc tggagctggt caatttaacc accctcacac cagactccaa
     2221 cacaaccact ggcctcagca cttccactgc cagtcctcat accagagcca acagcacctc
     2281 cacagagaga aagctgccgg agccggagag ccggggcgtg gtcatcgtgg ctgtgattgt
     2341 gtgcatcctg gtcctggcgg tgctgggcgc tgtcctctat ttcctctata agaagggcaa
     2401 gctgccgtgc aggcgctcag ggaagcagga gatcacgcta cccccgtctc gtaagagcga
     2461 acttgtagtt gaagttaagt cagataagct cccagaagag atgggcctcc tgcagggcag
     2521 cagcggtgac aagagggctc cgggagacca gggagagaaa tacatcgatc tgaggcatgg
     2581 tggaggcgga tcaggaggcg gtggatcgga tccaccggtc gccaccatgg tgagcaaggg
     2641 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2701 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2761 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2821 gacccacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2881 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2941 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3001 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3061 cttcaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3121 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3181 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3241 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3301 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     3361 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3421 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3481 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3541 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     3601 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3661 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3721 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3781 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3841 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3901 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3961 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4021 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     4081 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4141 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     4201 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4261 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4321 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4381 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4441 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4501 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4561 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4621 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4681 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4741 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4801 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4861 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4921 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4981 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5041 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5101 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5161 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5221 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5281 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5341 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5401 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5461 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5521 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5581 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5641 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5701 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5761 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5821 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5881 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5941 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6001 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6061 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6121 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6241 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6301 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6361 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6421 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6481 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6541 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6601 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6661 aaccgtatta ccgccatgca t
//
