LOCUS       V026545                 7126 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026545
VERSION     V026545
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..3034
                     /label="ITGB1(NM_002211)"
                     /note="ITGB1(NM_002211)"
                     /gene="ITGB1"
     CDS             3083..3793
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    3914..4035
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4042..4497)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4524..4628
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4630..4987
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5022..5816
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6048..6095
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6424..7012
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgaatttac aaccaatttt
      661 ctggattgga ctgatcagtt cagtttgctg tgtgtttgct caaacagatg aaaatagatg
      721 tttaaaagca aatgccaaat catgtggaga atgtatacaa gcagggccaa attgtgggtg
      781 gtgcacaaat tcaacatttt tacaggaagg aatgcctact tctgcacgat gtgatgattt
      841 agaagcctta aaaaagaagg gttgccctcc agatgacata gaaaatccca gaggctccaa
      901 agatataaag aaaaataaaa atgtaaccaa ccgtagcaaa ggaacagcag agaagctcaa
      961 gccagaggat attactcaga tccaaccaca gcagttggtt ttgcgattaa gatcagggga
     1021 gccacagaca tttacattaa aattcaagag agctgaagac tatcccattg acctctacta
     1081 ccttatggac ctgtcttact caatgaaaga cgatttggag aatgtaaaaa gtcttggaac
     1141 agatctgatg aatgaaatga ggaggattac ttcggacttc agaattggat ttggctcatt
     1201 tgtggaaaag actgtgatgc cttacattag cacaacacca gctaagctca ggaacccttg
     1261 cacaagtgaa cagaactgca ccagcccatt tagctacaaa aatgtgctca gtcttactaa
     1321 taaaggagaa gtatttaatg aacttgttgg aaaacagcgc atatctggaa atttggattc
     1381 tccagaaggt ggtttcgatg ccatcatgca agttgcagtt tgtggatcac tgattggctg
     1441 gaggaatgtt acacggctgc tggtgttttc cacagatgcc gggtttcact ttgctggaga
     1501 tgggaaactt ggtggcattg ttttaccaaa tgatggacaa tgtcacctgg aaaataatat
     1561 gtacacaatg agccattatt atgattatcc ttctattgct caccttgtcc agaaactgag
     1621 tgaaaataat attcagacaa tttttgcagt tactgaagaa tttcagcctg tttacaagga
     1681 gctgaaaaac ttgatcccta agtcagcagt aggaacatta tctgcaaatt ctagcaatgt
     1741 aattcagttg atcattgatg catacaattc cctttcctca gaagtcattt tggaaaacgg
     1801 caaattgtca gaaggcgtaa caataagtta caaatcttac tgcaagaacg gggtgaatgg
     1861 aacaggggaa aatggaagaa aatgttccaa tatttccatt ggagatgagg ttcaatttga
     1921 aattagcata acttcaaata agtgtccaaa aaaggattct gacagcttta aaattaggcc
     1981 tctgggcttt acggaggaag tagaggttat tcttcagtac atctgtgaat gtgaatgcca
     2041 aagcgaaggc atccctgaaa gtcccaagtg tcatgaagga aatgggacat ttgagtgtgg
     2101 cgcgtgcagg tgcaatgaag ggcgtgttgg tagacattgt gaatgcagca cagatgaagt
     2161 taacagtgaa gacatggatg cttactgcag gaaagaaaac agttcagaaa tctgcagtaa
     2221 caatggagag tgcgtctgcg gacagtgtgt ttgtaggaag agggataata caaatgaaat
     2281 ttattctggc aaattctgcg agtgtgataa tttcaactgt gatagatcca atggcttaat
     2341 ttgtggagga aatggtgttt gcaagtgtcg tgtgtgtgag tgcaacccca actacactgg
     2401 cagtgcatgt gactgttctt tggatactag tacttgtgaa gccagcaacg gacagatctg
     2461 caatggccgg ggcatctgcg agtgtggtgt ctgtaagtgt acagatccga agtttcaagg
     2521 gcaaacgtgt gagatgtgtc agacctgcct tggtgtctgt gctgagcata aagaatgtgt
     2581 tcagtgcaga gccttcaata aaggagaaaa gaaagacaca tgcacacagg aatgttccta
     2641 ttttaacatt accaaggtag aaagtcggga caaattaccc cagccggtcc aacctgatcc
     2701 tgtgtcccat tgtaaggaga aggatgttga cgactgttgg ttctatttta cgtattcagt
     2761 gaatgggaac aacgaggtca tggttcatgt tgtggagaat ccagagtgtc ccactggtcc
     2821 agacatcatt ccaattgtag ctggtgtggt tgctggaatt gttcttattg gccttgcatt
     2881 actgctgata tggaagcttt taatgataat tcatgacaga agggagtttg ctaaatttga
     2941 aaaggagaaa atgaatgcca aatgggacac gggtgaaaat cctatttata agagtgccgt
     3001 aacaactgtg gtcaatccga agtatgaggg aaaaggtgga ggcggatcag gaggcggtgg
     3061 atcggatcca ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
     3121 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     3181 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3241 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     3301 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3361 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     3421 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     3481 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     3541 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     3601 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3661 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     3721 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     3781 gctgtacaag tagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     3841 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3901 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3961 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4021 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4081 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4141 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4201 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4261 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4321 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4381 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4441 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     4501 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4561 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4621 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     4681 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4741 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4801 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4861 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4921 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4981 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5041 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5101 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5161 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5221 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5281 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5341 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5401 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5461 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5521 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     5581 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5641 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5701 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5761 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5821 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5881 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5941 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     6001 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     6061 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6121 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     6181 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     6241 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     6301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6541 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7081 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
