LOCUS V018218 5611 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018218 VERSION V018218 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 639..1451 /label="IL1A(NM_000575)" /note="IL1A(NM_000575)" /gene="IL1A" CDS 1557..2276 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2399..2520 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2527..2982) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3009..3113 /gene="bla" /label="AmpR promoter" promoter 3115..3472 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3507..4301 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4533..4580 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4909..5497 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctccaact ttgtgccaaa aagttggcat ggccaaagtt ccagacatgt 661 ttgaagacct gaagaactgt tacagtgaaa atgaagaaga cagttcctcc attgatcatc 721 tgtctctgaa tcagaaatcc ttctatcatg taagctatgg cccactccat gaaggctgca 781 tggatcaatc tgtgtctctg agtatctctg aaacctctaa aacatccaag cttaccttca 841 aggagagcat ggtggtagta gcaaccaacg ggaaggttct gaagaagaga cggttgagtt 901 taagccaatc catcactgat gatgacctgg aggccatcgc caatgactca gaggaagaaa 961 tcatcaagcc taggtcagca ccttttagct tcctgagcaa tgtgaaatac aactttatga 1021 ggatcatcaa atacgaattc atcctgaatg acgccctcaa tcaaagtata attcgagcca 1081 atgatcagta cctcacggct gctgcattac ataatctgga tgaagcagtg aaatttgaca 1141 tgggtgctta taagtcatca aaggatgatg ctaaaattac cgtgattcta agaatctcaa 1201 aaactcaatt gtatgtgact gcccaagatg aagaccaacc agtgctgctg aaggagatgc 1261 ctgagatacc caaaaccatc acaggtagtg agaccaacct cctcttcttc tgggaaactc 1321 acggcactaa gaactatttc acatcagttg cccatccaaa cttgtttatt gccacaaagc 1381 aagactactg ggtgtgcttg gcaggggggc caccctctat cactgacttt cagatactgg 1441 aaaaccaggc gtaccccact ttcttggaca gagttggcat tggcggtggc ggcagcggcg 1501 gaggcggaag cggaggcgga ggaagcccgc gggcccggga tccaccggtc gccaccatgg 1561 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 1621 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 1681 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 1741 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 1801 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 1861 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 1921 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 1981 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 2041 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 2101 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 2161 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 2221 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 2281 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 2341 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 2401 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 2461 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 2521 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 2581 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 2641 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 2701 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 2761 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 2821 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 2881 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 2941 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 3001 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3061 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 3121 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 3481 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 3541 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 3601 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 3661 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 3721 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 3781 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 3841 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 3901 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 3961 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 4021 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 4081 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 4141 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 4201 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 4261 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 4321 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 4381 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 4441 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 4501 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 4561 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 4621 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 4681 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 4741 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 4801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 4861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5041 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 5581 ttctgtggat aaccgtatta ccgccatgca t //