LOCUS       V046709                 6054 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046709
VERSION     V046709
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..1942
                     /label="ENO1(NM_001428)"
                     /note="ENO1(NM_001428)"
                     /gene="ENO1"
     CDS             2000..2719
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced YFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EYFP"
     polyA_signal    2842..2963
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2970..3425)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3452..3556
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3558..3915
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3950..4744
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4976..5023
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5352..5940
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atgtctattc tcaagatcca
      661 tgccagggag atctttgact ctcgcgggaa tcccactgtt gaggttgatc tcttcacctc
      721 aaaaggtctc ttcagagctg ctgtgcccag tggtgcttca actggtatct atgaggccct
      781 agagctccgg gacaatgata agactcgcta tatggggaag ggtgtctcaa aggctgttga
      841 gcacatcaat aaaactattg cgcctgccct ggttagcaag aaactgaacg tcacagaaca
      901 agagaagatt gacaaactga tgatcgagat ggatggaaca gaaaataaat ctaagtttgg
      961 tgcgaacgcc attctggggg tgtcccttgc cgtctgcaaa gctggtgccg ttgagaaggg
     1021 ggtccccctg taccgccaca tcgctgactt ggctggcaac tctgaagtca tcctgccagt
     1081 cccggcgttc aatgtcatca atggcggttc tcatgctggc aacaagctgg ccatgcagga
     1141 gttcatgatc ctcccagtcg gtgcagcaaa cttcagggaa gccatgcgca ttggagcaga
     1201 ggtttaccac aacctgaaga atgtcatcaa ggagaaatat gggaaagatg ccaccaatgt
     1261 gggggatgaa ggcgggtttg ctcccaacat cctggagaat aaagaaggcc tggagctgct
     1321 gaagactgct attgggaaag ctggctacac tgataaggtg gtcatcggca tggacgtagc
     1381 ggcctccgag ttcttcaggt ctgggaagta tgacctggac ttcaagtctc ccgatgaccc
     1441 cagcaggtac atctcgcctg accagctggc tgacctgtac aagtccttca tcaaggacta
     1501 cccagtggtg tctatcgaag atccctttga ccaggatgac tggggagctt ggcagaagtt
     1561 cacagccagt gcaggaatcc aggtagtggg ggatgatctc acagtgacca acccaaagag
     1621 gatcgccaag gccgtgaacg agaagtcctg caactgcctc ctgctcaaag tcaaccagat
     1681 tggctccgtg accgagtctc ttcaggcgtg caagctggcc caggccaatg gttggggcgt
     1741 catggtgtct catcgttcgg gggagactga agataccttc atcgctgacc tggttgtggg
     1801 gctgtgcact gggcagatca agactggtgc cccttgccga tctgagcgct tggccaagta
     1861 caaccagctc ctcagaattg aagaggagct gggcagcaag gctaagtttg ccggcaggaa
     1921 cttcagaaac cccttggcca aggaattcgg tggaggcgga tcaggaggcg gtggatctcc
     1981 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2041 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2101 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2161 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
     2221 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2281 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2341 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2401 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2461 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2521 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2581 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     2641 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2701 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     2761 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2821 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     2881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2941 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     3001 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3061 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3121 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3181 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     3241 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     3301 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     3361 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     3421 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3481 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3541 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     3601 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3661 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3721 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3781 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3841 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3901 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     3961 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4021 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4081 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     4141 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4201 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4261 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4321 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4381 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4441 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     4501 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4561 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4621 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4681 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4741 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     4801 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4861 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     4921 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     4981 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     5041 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     5101 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     5161 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     5221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5461 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
