LOCUS       V026099                 6895 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026099
VERSION     V026099
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             641..2818
                     /label="CCNT1(NM_001240)"
                     /note="CCNT1(NM_001240)"
                     /gene="CCNT1"
     CDS             2852..3562
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    3683..3804
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3811..4266)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4293..4397
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4399..4756
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4791..5585
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5817..5864
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6193..6781
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggagggag agaggaagaa
      661 caacaacaaa cggtggtatt tcactcgaga acagctggaa aatagcccat cccgtcgttt
      721 tggcgtggac ccagataaag aactttctta tcgccagcag gcggccaatc tgcttcagga
      781 catggggcag cgtcttaacg tctcacaatt gactatcaac actgctatag tatacatgca
      841 tcgattctac atgattcagt ccttcacaca gttccctgga aattctgtgg ctccagcagc
      901 cttgtttcta gcagctaaag tggaggagca gcccaaaaaa ttggaacatg tcatcaaggt
      961 agcacatact tgtctccatc ctcaggaatc ccttcctgat actagaagtg aggcttattt
     1021 gcaacaagtt caagatctgg tcattttaga aagcataatt ttgcagactt taggctttga
     1081 actaacaatt gatcacccac atactcatgt agtaaagtgc actcaacttg ttcgagcaag
     1141 caaggactta gcacagactt cttacttcat ggcaaccaac agcctgcatt tgaccacatt
     1201 tagcctgcag tacacacctc ctgtggtggc ctgtgtctgc attcacctgg cttgcaagtg
     1261 gtccaattgg gagatcccag tctcaactga cgggaagcac tggtgggagt atgttgacgc
     1321 cactgtgacc ttggaacttt tagatgaact gacacatgag tttctacaga ttttggagaa
     1381 aactcccaac aggctcaaac gcatttggaa ttggagggca tgcgaggctg ccaagaaaac
     1441 aaaagcagat gaccgaggaa cagatgaaaa gacttcagag cagacaatcc tcaatatgat
     1501 ttcccagagc tcttcagaca caaccattgc aggtttaatg agcatgtcaa cttctaccac
     1561 aagtgcagtg ccttccctgc cagtctccga agagtcatcc agcaacttaa ccagtgtgga
     1621 gatgttgccg ggcaagcgtt ggctgtcctc ccaaccttct ttcaaactag aacctactca
     1681 gggtcatcgg actagtgaga atttagcact tacaggagtt gatcattcct taccacagga
     1741 tggttcaaat gcatttattt cccagaagca gaatagtaag agtgtgccat cagctaaagt
     1801 gtcactgaaa gaataccgcg cgaagcatgc agaagaattg gctgcccaga agaggcaact
     1861 ggagaacatg gaagccaatg tgaagtcaca atatgcatat gctgcccaga atctcctttc
     1921 tcatcatgat agccattctt cagtcattct aaaaatgccc atagagggtt cagaaaaccc
     1981 cgagcggcct tttctggaaa aggctgacaa aacagctctc aaaatgagaa tcccagtggc
     2041 aggtggagat aaagctgcgt cttcaaaacc agaggagata aaaatgcgca taaaagtcca
     2101 tgctgcagct gataagcaca attctgtaga ggacagtgtt acaaagagcc gagagcacaa
     2161 agaaaagcac aagactcacc catctaatca tcatcatcat cataatcacc actcacacaa
     2221 gcactctcat tcccaacttc cagttggtac tgggaacaaa cgtcctggtg atccaaaaca
     2281 tagtagccag acaagcaact tagcacataa aacctatagc ttgtctagtt ctttttcctc
     2341 ttccagttct actcgtaaaa ggggaccctc tgaagagact ggaggggctg tgtttgatca
     2401 tccagccaag attgccaaga gtactaaatc ctcttcccta aatttctcct tcccttcact
     2461 tcctacaatg ggtcagatgc ctgggcatag ctcagacaca agtggccttt ccttttcaca
     2521 gcccagctgt aaaactcgtg tccctcattc gaaactggat aaagggccca ctggggccaa
     2581 tggtcacaac acgacccaga caatagacta tcaagacact gtgaatatgc ttcactccct
     2641 gctcagtgcc cagggtgttc agcccactca gcccactgca tttgaatttg ttcgtcctta
     2701 tagtgactat ctgaatcctc ggtctggtgg aatctcctcg agatctggca atacagacaa
     2761 accccggcca ccacctctgc catcagaacc tcctccacca cttccacccc ttcctaaggg
     2821 tggaggtggc tcggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
     2881 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     2941 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3001 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     3061 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     3121 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     3181 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     3241 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     3301 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3361 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3421 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3481 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3541 catggacgag ctgtacaagt agcggccgcg actctagatc ataatcagcc ataccacatt
     3601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3781 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3841 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3901 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3961 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4021 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     4081 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     4141 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     4201 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4261 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4321 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4381 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     4441 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4501 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4561 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4621 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4681 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4741 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4801 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4861 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4921 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4981 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5041 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5101 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5161 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5221 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5281 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     5341 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5401 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5461 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5521 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5581 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5641 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5701 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     5761 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     5821 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5881 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5941 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     6001 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     6061 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6181 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6241 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6301 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6361 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6421 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6481 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6541 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6601 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6661 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6721 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6781 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6841 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
