LOCUS       40924_11790        4807 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) library vector for low-background cloning and transient 
            mammalian cell expression of cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(125..259)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     664..680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    711..735
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             899..1201
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1239..1263)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(1264..1282)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1293..1309)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1435..1638)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1639..1942)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2402..2990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(3068..3101)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3206..4063)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(4351..4806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac
      661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactttgt
      721 acaagaaagc tgggtacgcg taagcttggg cccctcgagg gatcctctag agcggccgcc
      781 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      841 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg cttctagaat
      901 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
      961 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     1021 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     1081 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     1141 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     1201 ataagtcgac gatatcccgg gaattccgga ccggtaccag cctgcttttt tgtacaaact
     1261 tgttctatag tgtcacctaa ataggcctaa tggtcatagc tgtttcctgt gtgaaattgt
     1321 tatccgctcc gcggcctagg ctagagtccg gaggctggat cggtcccggt gtcttctatg
     1381 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct
     1441 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     1501 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     1561 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     1621 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     1681 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     1741 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     1801 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     1861 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     1921 atttaccgta agttatgtaa cgacatgcat ctaatgagtg aaagggcctc gtactacgcc
     1981 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     2041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaacg cgcgaattgc
     2161 aagctctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2581 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2821 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3061 tcatgccata acttcgtata gcatacatta tacgaagtta tggcatgaga ttatcaaaaa
     3121 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3181 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3241 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3301 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3361 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3421 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3481 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3541 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3601 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3661 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3721 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3781 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3841 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3901 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3961 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4021 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4081 attattgaag catttatcag ggttattgtc tcatgccagg ggtgggcaca catatttgat
     4141 accagcgatc cctacacagc acataattca atgcgacttc cctctatcgc acatcttaga
     4201 cctttattct ccctccagca cacatcgaag ctgccgagca agccgttctc accagtccaa
     4261 gacctggcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4321 cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat
     4381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     4441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     4501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     4621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     4681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     4741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4801 gcgcgtc
//
