LOCUS       Exported                4396 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Gateway(R) library vector for cloning and transient mammalian cell 
            expression of cDNAs. See pCMV SPORT6.1 for a newer version of this 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4396)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     cDNAs are typically cloned between the NotI and SalI sites.
FEATURES             Location/Qualifiers
     source          1..4396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    36..60
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(153..177)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(178..196)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(207..223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(349..552)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(553..856)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1316..1904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(1982..2015)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2120..2977)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(3265..3720)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(3846..3980)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ccagtgccta gcttataata cgactcacta tagggaccac tttgtacaag aaagctgggt
       61 acgcgtaagc ttgggcccct cgagggatac tctagagcgg ccgccgacta gtgagctcgt
      121 cgacgatatc ccgggaattc cggaccggta ccagcctgct tttttgtaca aacttgttct
      181 atagtgtcac ctaaataggc ctaatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      241 tcccgcggcc taggctagag tccggaggct ggatcggtcc cggtgtcttc tatggaggtc
      301 aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc actaaacgag ctctgcttat
      361 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      421 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      481 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      541 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      601 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
      661 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
      721 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
      781 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
      841 cgtaagttat gtaacgacat gcatctaatg cgtgaaaggg cctcgtaata cgcctatttt
      901 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1021 tgagacaata accctgataa atgcttcaat aatattgaaa aacgcgcgaa ttgcaagctc
     1081 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1201 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1261 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1321 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1381 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1441 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1501 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1561 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1621 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1681 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1741 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1801 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1861 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1921 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     1981 cataacttcg tatagcatac attatacgaa gttatggcat gagattatca aaaaggatct
     2041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2401 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2701 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3001 gaagcattta tcagggttat tgtctcatgc caggggtggg cacacatatt tgataccagc
     3061 gatccctaca cagcacataa ttcaatgcga cttccctcta tcgcacatct tagaccttta
     3121 ttctccctcc agcacacatc gaagctgccg agcaagccgt tctcaccagt ccaagacctg
     3181 gcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3241 catttccccg aaaagtgcca cctgaaattg taagcgttaa tattttgtta aaattcgcgt
     3301 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3361 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3481 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3601 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3661 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3721 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     3781 tattacgcca gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3841 ggatcgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3901 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3961 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4021 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     4081 atgatcatga acagactgtg aggactgagg ggcctgaaat gagccttggg actgtgaatc
     4141 taaaatacac aaacaattag aatcactagc tcctgtgtat aatattttca taaatcatac
     4201 tcagtaagca aaactctcaa gcagcaagca tatgcagcta gtttaacaca ttatacactt
     4261 aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc
     4321 acaccacaga agtaaggttc cttcacaaag atcccaagct agcagttttc ccagtcacga
     4381 cgttgtaaaa cgacgg
//
