LOCUS       pCMV_SPORT4.        4387 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for cloning and transient mammalian cell expression of cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV SPORT4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(125..259)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     664..680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    727..824
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(832..850)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(873..889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1015..1218)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1219..1522)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1982..2570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    complement(2648..2681)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2786..3643)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(3931..4386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac
      661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg agagagctat
      721 gacgtcgcat gcacgcgtaa gcttgggccc ctcgagggat cctctagagc ggccgccgac
      781 tagtgagctc gtcgacccgg gaattccgga ccggtacctg caggcgtacc ttctatagtg
      841 tcacctaaat agctttttgc aaaggcctaa tggtcatagc tgtttcctgt gtgaaattgt
      901 tatccgctcc gcggcctagg ctagagtccg gaggctggat cggtcccggt gtcttctatg
      961 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct
     1021 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     1081 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     1141 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     1201 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     1261 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     1321 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     1381 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     1441 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     1501 atttaccgta agttatgtaa cgacatgcat ctaatgagtg aaagggcctc gtactacgcc
     1561 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     1621 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1681 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaacg cgcgaattgc
     1741 aagctctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1801 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1861 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1921 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1981 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2041 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2101 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2161 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2221 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2281 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2341 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2401 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2461 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2521 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2581 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2641 tcatgccata acttcgtata gcatacatta tacgaagtta tggcatgaga ttatcaaaaa
     2701 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2761 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2821 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2881 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2941 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3001 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3061 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3121 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3181 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3241 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3301 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3361 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3421 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3481 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3541 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3601 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3661 attattgaag catttatcag ggttattgtc tcatgccagg ggtgggcaca catatttgat
     3721 accagcgatc cctacacagc acataattca atgcgacttc cctctatcgc acatcttaga
     3781 cctttattct ccctccagca cacatcgaag ctgccgagca agccgttctc accagtccaa
     3841 gacctggcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3901 cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat
     3961 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     4021 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     4081 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     4141 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     4201 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     4261 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     4321 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4381 gcgcgtc
//
