LOCUS       V028249                 4523 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028249
VERSION     V028249
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..1608
                     /label="SNRPA1(NM_003090)"
                     /note="SNRPA1(NM_003090)"
                     /gene="SNRPA1"
     CDS             1624..1647
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    1662..1796
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1907..1923)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1931..1947
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1955..1985)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2000..2021
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2309..2897)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3068..3928)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3929..4033)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4507..4523
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatggtca agctgacggc ggagctgatc gagcaggcgg cgcagtacac caacgcggtg
      901 cgcgaccggg agctggacct ccgggggtat aaaattcccg tcattgaaaa tctaggtgct
      961 acgttagacc agtttgatgc tattgatttt tctgacaatg agatcaggaa actggatggt
     1021 tttcctttgt tgagaagact gaaaacattg ttagtgaaca acaacagaat atgccgtata
     1081 ggtgagggac ttgatcaggc tctgccctgt ctgacagaac tcattctcac caataatagt
     1141 ctcgtggaac tgggtgatct ggaccctctg gcatctctca aatcgctgac ttacctaagt
     1201 atcctaagaa atccggtaac caataagaag cattacagat tgtatgtgat ttataaagtt
     1261 ccgcaagtca gagtactgga tttccagaaa gtgaaactaa aagagcgtca ggaagcagag
     1321 aaaatgttca agggcaaacg gggtgcacag cttgcaaagg atattgccag gagaagcaaa
     1381 acttttaatc caggtgctgg tttgccaact gacaaaaaga aaggtgggcc atctccaggg
     1441 gatgtagaag caatcaagaa tgccatagca aatgcttcaa ctctggctga agtggagagg
     1501 ctgaaggggt tgctgcagtc tggtcagatc cctggcagag aacgcagatc agggcccact
     1561 gatgatggtg aagaagagat ggaagaagac acagtcacaa acgggtccac tcgaggtacc
     1621 ggcgactaca aggacgacga tgacaagtaa gcggccgcgg ggatccagac atgataagat
     1681 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     1741 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     1801 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcgg
     1861 atcctctaga gtcgatctgc aggcatgcta gcttggcgta atcatggtca tagctgtttc
     1921 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1981 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2041 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2101 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2161 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2221 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2281 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2341 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2401 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2461 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2521 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2581 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2641 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2701 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2761 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2821 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2881 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2941 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3001 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3061 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4021 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4081 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     4141 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     4201 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4261 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4321 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     4381 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4441 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4501 gacgttgtaa aacgacggcc agt
//
